From research lab to standard environmental analysis tool: Will NASBA make the leap?

Hønsvall, Birgitte K.; Robertson, Lucy J.

doi:10.1016/j.watres.2016.11.052

Cited by 49 publications

(30 citation statements)

References 56 publications

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“…Nucleic acid sequence-based amplification (NASBA) is an isothermal method that has been proposed as an efficient diagnostic approach for amplifying RNA targets of viable cells and adapted in clinical diagnostics [116]. The NASBA method to detect live tachyzoites of T. gondii in the peritoneal cavities of mice based on the amplification of the tachyzoites' B1 rRNA gene has been developed [117].…”

Section: Nucleic Acid Sequence-based Amplificationmentioning

confidence: 99%

Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals

Khan

Noordin

2019

Eur J Clin Microbiol Infect Dis

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Infection by Toxoplasma gondii is prevalent worldwide. The parasite can infect a broad spectrum of vertebrate hosts, but infection of fetuses and immunocompromised patients is of particular concern. Easy-to-perform, robust, and highly sensitive and specific methods to detect Toxoplasma infection are important for the treatment and management of patients. Rapid diagnostic methods that do not sacrifice the accuracy of the assay and give reproducible results in a short time are highly desirable. In this context, rapid diagnostic tests (RDTs), especially with point-of-care (POC) features, are promising diagnostic methods in clinical microbiology laboratories, especially in areas with minimal laboratory facilities. More advanced methods using microfluidics and sensor technology will be the future trend. In this review, we discuss serological and molecular-based rapid diagnostic tests for detecting Toxoplasma infection in humans as well as animals.

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Section: Nucleic Acid Sequence-based Amplificationmentioning

confidence: 99%

Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals

Khan

Noordin

2019

Eur J Clin Microbiol Infect Dis

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“…which may be obtained from Equation (3) by first multiplying numerator and denominator by the factor ( e −kt 50 · e kt ) , and then representing the resultant fraction in the form of a Maclaurin series. Approximating this series by the dominant zero-and first-order terms yields Equation (4). As discussed in the next section, we also require that t 50 ≫ 0 as well, so that e kt 50 ≫ 1.…”

Section: Modeling and Parameter Estimation From Experimental Datamentioning

confidence: 99%

“…A diverse set of techniques has been proposed for nucleic acid amplification based on isothermal processes, including loop‐mediated isothermal amplification (LAMP), rolling circle amplification (RCA), recombinase polymerase amplification (RPA), nucleic acid sequence–based amplification (NASBA), and others . Compared to amplification techniques that rely on thermally‐cycled processes (eg, polymerase chain reaction (PCR)), isothermal amplification protocols confer a significant advantage due to the relative technical simplicity in the instrumentation used to implement the processes.…”

Section: Introductionmentioning

confidence: 99%

Mathematical modeling of a real‐time isothermal amplification assay for Erwinia amylovora

Gordon

Klemer²,

Fuller

et al. 2019

Engineering Reports

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A general mathematical model that describes the temporal behavior of a real‐time isothermal process used for nucleic acid amplification is derived. A monotonically‐increasing fluorescence signal s(t) generated and measured during the amplification reaction can be modeled in the form of a logistic function of time that is completely described by three parameters (k, t50, and Smax), which may be readily estimated from experimentally acquired s(t) data. Experimental data obtained from a real‐time loop‐mediated isothermal amplification (LAMP) assay for the infectious pathogen Erwinia amylovora (E. amylovora) are used to illustrate and validate the mathematical model. Implementation of such a modeling approach can allow for the extraction of quantitative information from real‐time LAMP data through parameter estimation techniques; this is demonstrated experimentally using real‐time amplification data acquired using the real‐time E. amylovora assay.

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“…Several exponential amplification techniques that do not require thermal cycling have been developed. [4][5][6] Some commonly used isothermal exponential amplification techniques include loop-mediated isothermal amplification (LAMP), [7][8][9][10][11][12][13][14][15] exponential rolling circle amplification (E-RCA), [16][17][18][19][20] exponential strand displacement amplification (E-SDA), [21][22][23] helicase-dependent amplification (HDA), [24][25][26][27] recombinase polymerase amplification (RPA), [28][29][30][31][32][33][34] nucleic acid sequence based amplification (NASBA), [35][36][37][38][39] nicking and extension amplification reaction (NEAR), [40] and exponential amplification reaction (EXPAR). [41] All these exponential DNAamplification techniques require the generation of as sDNAt emplate from ad sDNAa mplicon to enable template-guided DNAelongation in each amplification cycle.…”

Section: Introductionmentioning

confidence: 99%

Exponential Isothermal Amplification of Nucleic Acids and Assays for Proteins, Cells, Small Molecules, and Enzyme Activities: An EXPAR Example

2018

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Isothermal exponential amplification techniques, such as strand-displacement amplification (SDA), rolling circle amplification (RCA), loop-mediated isothermal amplification (LAMP), nucleic acid sequence based amplification (NASBA), helicase-dependent amplification (HDA), and recombinase polymerase amplification (RPA), have great potential for on-site, point-of-care, and in situ assay applications. These amplification techniques eliminate the need for temperature cycling, as required for the polymerase chain reaction (PCR), while achieving comparable amplification yields. We highlight here recent advances in the exponential amplification reaction (EXPAR) for the detection of nucleic acids, proteins, enzyme activities, cells, and metal ions. The incorporation of fluorescence, colorimetric, chemiluminescence, Raman, and electrochemical approaches enables the highly sensitive detection of a variety of targets. Remaining issues, such as undesirable background amplification resulting from nonspecific template interactions, must be addressed to further improve isothermal and exponential amplification techniques.

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From research lab to standard environmental analysis tool: Will NASBA make the leap?

Cited by 49 publications

References 56 publications

Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals

Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals

Mathematical modeling of a real‐time isothermal amplification assay for Erwinia amylovora

Exponential Isothermal Amplification of Nucleic Acids and Assays for Proteins, Cells, Small Molecules, and Enzyme Activities: An EXPAR Example

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