From the proteomic point of view: Integration of adaptive changes to iron deficiency in plants

Mai, Hans-Jörg; Bauer, Petra

doi:10.1016/j.cpb.2016.02.001

Cited by 26 publications

(16 citation statements)

References 120 publications

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“…Comparative proteomics can provide useful information not only about processes occurring in xylem sap during the adaptation to nutrient stresses, but also can help to target proteins putatively involved in systemic regulation for future studies [47,48]. A significant number of proteomic studies have focused into the effect on different sub-proteomes of nutrient stresses such as Fe (see [49,50] for reviews) and Mn [51,52], but the number of proteomic studies devoted to the effect of nutrient deficiencies and/or toxicities in the xylem sap has been limited, to the best of our knowledge, to studies on the effects of salt stress, K deficiency and N starvation or overload [53][54][55].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Effects of Fe and Mn deficiencies on the protein profiles of tomato ( Solanum lycopersicum ) xylem sap as revealed by shotgun analyses

Ceballos-Laita

Gutiérrez-Carbonell

Takahashi

et al. 2018

Journal of Proteomics

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The aim of this work was to study the effects of Fe and Mn deficiencies on the xylem sap proteome of tomato using a shotgun proteomic approach, with the final goal of elucidating plant response mechanisms to these stresses. This approach yielded 643 proteins reliably identified and quantified with 70% of them predicted as secretory. Iron and Mn deficiencies caused statistically significant and biologically relevant abundance changes in 119 and 118 xylem sap proteins, respectively. In both deficiencies, metabolic pathways most affected were protein metabolism, stress/oxidoreductases and cell wall modifications. First, results suggest that Fe deficiency elicited more stress responses than Mn deficiency, based on the changes in oxidative and proteolytic enzymes. Second, both nutrient deficiencies affect the secondary cell wall metabolism, with changes in Fe deficiency occurring via peroxidase activity, and in Mn deficiency involving peroxidase, Cu-oxidase and fasciclin-like arabinogalactan proteins. Third, the primary cell wall metabolism was affected by both nutrient deficiencies, with changes following opposite directions as judged from the abundances of several glycoside-hydrolases with endo-glycolytic activities and pectin esterases. Fourth, signaling pathways via xylem involving CLE and/or lipids as well as changes in phosphorylation and N-glycosylation also play a role in the responses to these stresses. Biological significance In spite of being essential for the delivery of nutrients to the shoots, our knowledge of xylem responses to nutrient deficiencies is very limited. The present work applies a shotgun proteomic approach to unravel the effects of Fe and Mn deficiencies on the xylem sap proteome. Overall, Fe deficiency seems to elicit more stress in the xylem sap proteome than Mn deficiency, based on the changes measured in proteolytic and oxido-reductase proteins, whereas both nutrients exert modifications in the composition of the primary and secondary cell wall. Cell wall modifications could affect the mechanical and permeability properties of the xylem sap vessels, and therefore ultimately affect solute transport and distribution to the leaves. Results also suggest that signaling cascades involving lipid and peptides might play a role in nutrient stress signaling and pinpoint interesting candidates for future studies. Finally, both nutrient deficiencies seem to affect phosphorylation and glycosylation processes, again following an opposite pattern.

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Section: Accepted Manuscriptmentioning

confidence: 99%

Effects of Fe and Mn deficiencies on the protein profiles of tomato ( Solanum lycopersicum ) xylem sap as revealed by shotgun analyses

Ceballos-Laita

Gutiérrez-Carbonell

Takahashi

et al. 2018

Journal of Proteomics

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“…Iron (Fe) is one of the essential micronutrients for plant growth and development and is required for a wide range of biochemical processes from photosynthesis to respiration. Fe is essential for maintaining the chloroplast structure and function, for biosynthesis of Fe-S clusters and chlorophyll (Chl), and is involved in the electron transport systems (Broadley et al, 2012;Briat et al, 2015;Mai et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Nano iron (Fe) complex is an effective source of Fe for tobacco plants grown under low Fe supply

Bastani

Hajiboland

Khatamian

et al. 2018

J. Soil Sci. Plant Nutr.

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In order to compare the uptake, utilization and distribution of iron (Fe) as nano and bulk Fe complex (Fe(III)-EDTA), an experiment was conducted using hydroponically-grown Fe-deficient tobacco (Nicotiana rustica L.) plants. Plants were treated with Fe either through roots (root application, RA) or leaves (foliar application, FA).Leaf chlorophyll concentration and plants biomass responded to the Fe re-supply; this response was quicker for the nano Fe complex than the bulk Fe complex, in particular, in the RA plants. Plants re-supplied with the nano Fe complex had lower Fe content but higher Fe use efficiency than plants re-supplied with the bulk Fe complex.Analysis of different plant fractions at two subsequent weeks revealed that the nano Fe complex had higher mobility than the bulk Fe complex, both in the xylem and the phloem. Comparison of RA and FA revealed that the re-supply of Fe through roots was more efficient in retrieving the whole plant growth than the foliar Fe spray.RA, in contrast to FA plants, profited from an enhanced Fe uptake capacity induced under Fe starvation in the roots. Alternatively, FA was effective in extending the green leaf area duration. Our data suggested that nano Fe complex is advantageous as both leaf spray and a long-term feeding of plant through roots.

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“…These are enzymes that help in the conversion of H 2 O 2 (hydrogen peroxide) to H 2 O (water) and oxygen. In addition, Fe deficiency in the roots of M. truncatula (Rodríguez-Celma et al, 2011) and P. dulcis × P. persica (Rodriguez-Celma et al, 2013) was characterized by the superoxide dismutase, i.e., ATMSD1 (ARABIDOP-SIS MANGANESE SUPEROXIDE DISMUTASE 1; AT3G10920) (Mai and Bauer, 2016). Furthermore, nonenzymatic ROS generation also occurs due to Fe deficiency.…”

Section: Rapeseed (Brassica Napus)mentioning

confidence: 99%

“…Although high concentrations of Fe can lead to toxic consequences in plants (Anjum et al, 2015). Excess free Fe ions like Fe 2+ and Fe 3+ can cause ROS generation by participating in the Fenton reaction (Fenton, 1894;Haber and Weiss, 1934;Kehrer, 2000;Mai and Bauer, 2016) and leading to oxidative stress (Mai and Bauer, 2016). Down regulated detoxifying proteins in Fe-deprived conditions, viz.…”

Section: Rapeseed (Brassica Napus)mentioning

confidence: 99%

Acquisition and Homeostasis of Iron in Higher Plants and Their Probable Role in Abiotic Stress Tolerance

Tripathi

Singh

Gaur

et al. 2018

Front. Environ. Sci.

167

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Iron (Fe) is a micronutrient that plays an important role in agriculture worldwide because plants require a small amount of iron for its growth and development. All major functions in a plant's life from chlorophyll biosynthesis to energy transfer are performed by Fe (Brumbarova et al., 2008;Gill and Tuteja, 2011). Iron also acts as a major constituent of many plant proteins and enzymes. The acquisition of Fe in plants occurs through two strategies, i.e., strategy I and strategy II (Marschner and Römheld, 1994). Under various stress conditions, Nramp and the YSL gene families help in translocation of Fe, which further acts as a mineral regulatory element and defends plants against stresses. Iron plays an irreplaceable role in alleviating stress imposed by salinity, drought, and heavy metal stress. This is because it activates plant enzymatic antioxidants like catalase (CAT), peroxidase, and an isoform of superoxide dismutase (SOD) that act as a scavenger of reactive oxygen species (ROS) (Hellin et al., 1995). In addition to this, their deficiency as well as their excess amount can disturb the homeostasis of a plant's cell and result in declining of photosynthetic rate, respiration, and increased accumulation of Na + and Ca − ions which culminate in an excessive formation of ROS. The short-range order hydrated Fe oxides and organic functional groups show affinities for metal ions. Iron plaque biofilm matrices could sequester a large amount of metals at the soil-root interface. Hence, it has attracted the attention of plant physiologists and agricultural scientists who are discovering more exciting and hidden applications of Fe and its potential in the development of bio-factories. This review looks into the recent progress made in putting forward the role of Fe in plant growth, development, and acclimation under major abiotic stresses, i.e., salinity, drought, and heavy metals.

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From the proteomic point of view: Integration of adaptive changes to iron deficiency in plants

Cited by 26 publications

References 120 publications

Effects of Fe and Mn deficiencies on the protein profiles of tomato ( Solanum lycopersicum ) xylem sap as revealed by shotgun analyses

Effects of Fe and Mn deficiencies on the protein profiles of tomato ( Solanum lycopersicum ) xylem sap as revealed by shotgun analyses

Nano iron (Fe) complex is an effective source of Fe for tobacco plants grown under low Fe supply

Acquisition and Homeostasis of Iron in Higher Plants and Their Probable Role in Abiotic Stress Tolerance

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