Due to the overuse of fossil energy, fossil energy is facing exhaustion. Agricultural biomass such as wheat straw waste is a kind of clean energy which can replace fossil energy and has important scientific research value and broad application prospect. In this paper, wheat straw was used as raw materials, and the straw was converted into lactic acid, oxalic acid and fumaric acid under alkaline hydrothermal conditions. Improve the yield of organic acid by optimizing the reaction conditions such as NaOH concentration, reaction time and reaction temperature. The optimum reaction conditions were as follows: NaOH concentration 1.5 mol/L, reaction temperature 250ºC, reaction time 3 hours, total organic acid yield 13.5%, lactic acid yield 12.1%. The results can provide a new way for the green production of organic acids, and realize the efficient utilization of agricultural waste, reduce environmental pollution. At the same time, can produce good economic benefits, ecological benefits and social benefits.