Frozen assembly of gold nanoparticles for rapid analysis of antifreeze protein activity

Park, Ji-In; Lee, Jun Hyuck; Gwak, Yunho; Kim, Soo Hyun; Jin, EonSeon; Kim, Young‐Pil

doi:10.1016/j.bios.2012.09.052

Cited by 16 publications

(11 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To test the applicability, if any, of the reversible freezing of colloidal gold as a probe for IRI activity (rather than the previously explored thermal hysteresis) 22 a range of synthetic polymers with known IRI activity were selected. Poly(vinyl alcohol), PVA, was chosen as the positive control due to is well-known IRI activity, which has been investigated in detail 28 .…”

Section: Resultsmentioning

confidence: 99%

“…30 nm mercaptosuccinic acid (MSA) functionalized gold particles prepared using literature methods 22 32 . The gold nanoparticle solutions containing the compounds to be tested for IRI were frozen in 96-well plates at −20 °C for 2 hours in a standard laboratory freezer (−20 °C).…”

Section: Resultsmentioning

confidence: 99%

“…Interestingly, Kim et al 22 , suggested that gold nanoparticles could be used to measure thermal hysteresis colourimetrically. Gold nanoparticles show a distinct shift from red to blue colouration upon aggregation and has been employed for a range of bioassays from DNA-sequence comparison 23 , bacteria analysis 24 , viral analysis 25 , glycan analysis 26 , metal detection 27 and more.…”

mentioning

confidence: 99%

“…Gold nanoparticles show a distinct shift from red to blue colouration upon aggregation and has been employed for a range of bioassays from DNA-sequence comparison 23 , bacteria analysis 24 , viral analysis 25 , glycan analysis 26 , metal detection 27 and more. Kim et al 22 reported that the aggregation of gold particles correlated with the TH activity of an AFP. The mechanism of this was not clear though as freezing then thawing gold nanoparticles should not actually be affected by TH.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Gold Nanoparticle Aggregation as a Probe of Antifreeze (Glyco) Protein-Inspired Ice Recrystallization Inhibition and Identification of New IRI Active Macromolecules

Mitchell

Congdon

Rodger

et al. 2015

Sci Rep

View full text Add to dashboard Cite

Antifreeze (glyco)proteins are found in polar fish species and act to slow the rate of growth of ice crystals; a property known as ice recrystallization inhibition. The ability to slow ice growth is of huge technological importance especially in the cryopreservation of donor cells and tissue, but native antifreeze proteins are often not suitable, nor easily available. Therefore, the search for new materials that mimic this function is important, but currently limited by the low-throughout assays associated with the antifreeze properties. Here 30 nm gold nanoparticles are demonstrated to be useful colorimetric probes for ice recrystallization inhibition, giving a visible optical response and is compatible with 96 well plates for high-throughout studies. This method is faster, requires less infrastructure, and has easier interpretation than the currently used ‘splat’ methods. Using this method, a series of serum proteins were identified to have weak, but specific ice recrystallization inhibition activity, which was removed upon denaturation. It is hoped that high-throughput tools such as this will accelerate the discovery of new antifreeze mimics.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Gold Nanoparticle Aggregation as a Probe of Antifreeze (Glyco) Protein-Inspired Ice Recrystallization Inhibition and Identification of New IRI Active Macromolecules

Mitchell

Congdon

Rodger

et al. 2015

Sci Rep

View full text Add to dashboard Cite

show abstract

“…We chose the AFP from the Antarctic marine diatom Chaetoceros neogracile ( Cn- AFP). Not only can this protein be produced in large amounts via expression in Escherichia coli , it also exhibits great potential to lower the freezing point at a relatively low concentration in comparison with other AFPs 15 16 17 . In the present study, we introduce metal binding peptides fused to an AFP to effectively adsorb the Cn- AFP on Al surfaces, which allows for rapid immobilization of AFPs on the metal substrate.…”

mentioning

confidence: 99%

Creating Anti-icing Surfaces via the Direct Immobilization of Antifreeze Proteins on Aluminum

Gwak

Park

Kim

et al. 2015

Sci Rep

Self Cite

View full text Add to dashboard Cite

Cryoprotectants such as antifreeze proteins (AFPs) and sugar molecules may provide a solution for icing problems. These anti-icing substances protect cells and tissues from freezing by inhibiting ice formation. In this study, we developed a method for coating an industrial metal material (aluminum, Al) with AFP from the Antarctic marine diatom, Chaetoceros neogracile (Cn-AFP), to prevent or delay ice formation. To coat Al with Cn-AFP, we used an Al-binding peptide (ABP) as a conjugator and fused it with Cn-AFP. The ABP bound well to the Al and did not considerably change the functional properties of AFP. Cn-AFP-coated Al (Cn-AFP-Al) showed a sufficiently low supercooling point. Additional trehalose coating of Cn-AFP-Al considerably delayed AFP denaturation on the Al without affecting its antifreeze activity. This metal surface–coating method using trehalose-fortified AFP can be applied to other metals important in the aircraft and cold storage fields where anti-icing materials are critical.

show abstract

Mechanisms, Applications, and Challenges of Utilizing Nanomaterials in Cryopreservation

Wang,

Gao,

Shu

2024

Adv Eng Mater

View full text Add to dashboard Cite

Cryopreservation of biological samples, including cells, tissues, and organs, has become an essential component in various biomedical research and applications, such as cellular therapy, tissue engineering, organ transplantation, and conservation of endangered species. However, it faces critical challenges throughout the cryopreservation process, such as loading/unloading of cryoprotective agent (CPA), ice inhibition during cooling, and ultrafast and uniform heating during rewarming. Applying nanomaterials in cryopreservation has emerged as a promising solution to address these challenges in each step due to their unique properties. For instance, in order to deliver nonpermeating CPA into cells, some nanomaterials, such as polymeric nanocapsule, can carry nonpermeating CPA to penetrate into the cells, regulating the intracellular ice crystal. During cooling, some nanomaterials, such as graphene oxide, can bind to basal or prism planes of ice crystals, suppressing the ice growth. During rewarming, some nanomaterials, such as magnetic nanoparticles, can improve the heating performance, preventing devitrification and recrystallization during rewarming. However, challenges in nanomaterials‐assisted cryopreservation remain, including the need for comprehensive studies on nanomaterials toxicity and the development of scalable manufacturing processes for industrial applications. This review examines the role of nanomaterials in cryopreservation, focusing on their mechanisms, applications, and associated challenges.

show abstract

Frozen assembly of gold nanoparticles for rapid analysis of antifreeze protein activity

Cited by 16 publications

References 25 publications

Gold Nanoparticle Aggregation as a Probe of Antifreeze (Glyco) Protein-Inspired Ice Recrystallization Inhibition and Identification of New IRI Active Macromolecules

Gold Nanoparticle Aggregation as a Probe of Antifreeze (Glyco) Protein-Inspired Ice Recrystallization Inhibition and Identification of New IRI Active Macromolecules

Creating Anti-icing Surfaces via the Direct Immobilization of Antifreeze Proteins on Aluminum

Mechanisms, Applications, and Challenges of Utilizing Nanomaterials in Cryopreservation

Contact Info

Product

Resources

About