Frozen Blood

Cr, Valeri

doi:10.1056/nejm196608252750806

Cited by 9 publications

(5 citation statements)

References 16 publications

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“…Our m a j o r problem was control of t h e amount of blood d r a w n a t a n y one time and, thereby, control of t h e volume of red blood cells obtained for freezing. Since 90.6 f 5.6 (n = 14) both the size of the freezing container and the capacity of the washing bowl limit the amount of red blood cells that can be frozen, thawed, and washed at any one time, precise control of the volume of blood drawn is essential.…”

Section: Discussionmentioning

confidence: 99%

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Recovery of Glycerolized Red Blood Cells Frozen in Liquid Nitrogen

Runck

Valeri

1969

Transfusion

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Human red blood cells were frozen at a rapid rate in the presence of approximately 20 per cent w/v glycerol and an osmotic supporting agent, and were stored in liquid nitrogen vapor at approximately -150 C for at least two weeks before thawing, washing, and resuspension. The recovery of red blood celb upon thawing was dependent on the type of freezing container wed and the volume of red blood cells frozen. An additional loss of red blood celb during postthaw washing was related to the volume of red blood cells frozen, but was not related to the type of osmotic supporting agent used during the wash. The total amount of aupanatant hemoglobin in the washed unit was inversely related to the recovery of the red blood cells upon thawing. For adequate recovery of preserved red blood cells, both the geometry of the container in which the cells had been frozen, and the capacity of the reusable bowl-washing system employed for removing the glycerol, limited the m m of glycerolized red blood celh to less than 600 g.

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Section: Discussionmentioning

confidence: 99%

“…cent (n =14) with the Pert method. T h e total mass of glycerolized red blood cells prior to freezing ranged from 450 to 700 g per unit in both methods.…”

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confidence: 99%

Recovery of Glycerolized Red Blood Cells Frozen in Liquid Nitrogen

Runck

Valeri

1969

Transfusion

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“…During the freezing process, penetrative cryoprotectants increase output of intracellular water, maintaining the osmotic balance in a partially frozen extracellular solution in this way. It results in not only reducing the cells' volume but also in the reduction of the osmotic load [1,[5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. Extracellular (nonpenetrative) cryoprotectants are macromolecular substances and due to their molecular mass, they do not penetrate cellular membrane and are mostly used for rapid and ultra-rapid freezing.…”

Section: Methods Of Cryopreservation Of Blood Cellsmentioning

confidence: 99%

Cryopreservation of Platelets: Advances and Current Practice

Bohoněk¹

2018

Cryopreservation Biotechnology in Biomedical and Biological Sciences

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Conventional fresh platelets stored at 20-24°C have a short shelf life, at most 7 days. Their main disadvantage is logistics as it is more difficult. This limitation is especially problematic for emergency and intensive care departments for managing massive bleeding. The early and aggressive use of blood products for massive hemorrhage may correct coagulopathy, control bleeding, and improve outcomes. The timely availability of platelets at the shortest time after the injury is often problematic. Many hospitals cannot afford to have platelets permanently in stock because of its short shelf life. Cryopreservation and storage of frozen platelets may significantly prolong their shelf life. Thus, frozen platelets provide long-term accessibility in situations where fresh products are not available. The most widely used method for the platelets cryopreservation is freezing at 5-6% DMSO at −80°C. The production of cryopreserved platelets is not technologically demanding, they can be easily thawed and reconstituted. Frozen platelets are an alternative blood product for urgent orders in connection with heavy bleeding. They are cost-effective functional platelets product for the management of bleeding and should be considered for wider use in clinical practice, such as autologous platelets, rare or HLA/HPA compatible platelets and platelets for non-transfusion use.

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“…It results in not only reducing the cells' volume but also in the reduction of the osmotic load. With regard to subsequent survival of cells after their thawing, a significant effect is the inhibition of APT caused by some of the cryoprotectants such as glycerol and DMSO [15,23].…”

Section: Intracellular (Penetrative) Cryoprotectantsmentioning

confidence: 99%

Cryopreservation of Blood

Bohoněk¹

2012

Blood Transfusion in Clinical Practice

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Frozen Blood

Cited by 9 publications

References 16 publications

Recovery of Glycerolized Red Blood Cells Frozen in Liquid Nitrogen

Recovery of Glycerolized Red Blood Cells Frozen in Liquid Nitrogen

Cryopreservation of Platelets: Advances and Current Practice

Cryopreservation of Blood

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