Frozen-thawed Sperm Motility Characteristics of African Catfish (Clarias gariepinus) by Using Glycerol or DMSO Based Extender

Kamaruding, Noor Azlina; Embong, Wan Khadijah Wan; Abdullan, Ramli Bin

doi:10.7763/ijesd.2012.v3.186

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…Extender is a salt solution which helps to maintain the viability of cell during cryopreservation, which supplies sources of energy to sperm cells, protect the cells from temperature related damage, and maintain a suitable environment for the sperm to survive during the period of cryopreservation [76,77]. Based on the inorganic composition of seminal plasma, extender is prepared as a buffered physiological saline solution [78] and hence extender composition differs between species.…”

Section: Extendermentioning

confidence: 99%

Cryopreservation and Its Application in Aquaculture

C¹,

Siva²,

J³

2022

Veterinary Medicine and Science

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Aquaculture is the major aquatic animal production segment. Problems like inbreeding depression, genetic drift, introgressive hybridization, etc. have been influencing the production of quality seeds negatively. Cryopreservation serves as a way-out for these problems and a possible answer to produce quality seeds and genetically improved varieties. It has been considered as a major strategy for conservation of fish genetic resources. Cryopreservation of fish gametes has been in vogue since 1953 and the technology is well studied and validated for many species. So far the milt of 200 fish species has been cryopreserved successfully. In this chapter, the importance of aquaculture in overcoming malnutrition, genetic issues affecting quality seed production, cryopreservation protocol employed for various fish species, problems faced in cryopreserving fish eggs and embryos and future of cryopreservation in aquaculture have been discussed.

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Section: Extendermentioning

confidence: 99%

Cryopreservation and Its Application in Aquaculture

C¹,

Siva²,

J³

2022

Veterinary Medicine and Science

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“…Other than aspect of quantitative production, it is necessary to consider sperm quality and the amount of sperm available for fertilization. Fresh sperm assessment is a useful step to indicate the success of cryopreservation technique as well as giving the baseline information on the fecundity of African catfish (Azlina et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Reproductive Tract Morphometry and Evaluation of Fresh Wild African ‎Catfish (Clarias gariepinus) Milt from Lake Alau, Maiduguri, Nigeria‎

Ali

Asuku

Bamanga

et al. 2022

SJVS

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Reproductive morphometry of Clarias gariepinus (C. gariepinus) is essential in distinguishing different species that are closely related to it and adequate information is required regarding the seasonal variation of milt characteristic of wild species. This study was designed to document the reproductive tract morphometric dimensions and milt characteristics of wild C. gariepinus during spawning and non-spawning seasons in Lake Alau, Maiduguri, Nigeria. Sixty male brood stocks were used comprising thirty each for spawning and non-spawning seasons. The reproductive tracts were removed and their dimensions measured. The milt samples were collected, pooled and evaluated. Results of the study showed that the body weight, sperm concentration and testicular indices differed significantly (p˂0.05) between the two seasons of evaluation. In addition, a positive correlation (p˂ 0.05) between body weight of fish and milt volume (r = 0.656 and r = 0.646) was also observed in both seasons. It was concluded that the left testis of C. gariepinus is heavier, longer and thicker than the right. Nonetheless, the C. gariepinus had larger body weights and higher spermatozoa concentration in spawning season than in non-spawning season; those with larger body weights had higher volume of milt than those with lower body weights.

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“…Portanto, busca-se a associação ideal entre esses elementos com o intuito de garantir uma boa qualidade seminal pós-descongelação (8)(9)(10) . Diluentes mais complexos com diferentes concentrações de íons podem fornecer maior nutrição e proteção aos espermatozoides, como o Ringer modificado para peixes, composto por NaCl, KCl, NaHCO 3 e CaCl 2 , já testado para outras espécies de água doce, apresentando boas taxas de motilidade espermática (11)(12)(13) . De acordo com Salmito-Vanderley et al (8) , um dos crioprotetores intracelulares mais utilizados com sucesso na congelação seminal de peixes é o dimetilsulfóxido (DMSO), já testado para várias espécies de Characiformes (14)(15)(16)(17) .…”

Section: Introductionunclassified

QUALIDADE DO SÊMEN DE TAMBAQUI (Colossoma macropomum) CRIOPRESERVADO EM DIFERENTES CONCENTRAÇÕES DE GEMA DE OVO

et al. 2016

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Resumo O objetivo deste trabalho foi avaliar o efeito da adição de gema de ovo (GO) sobre a cinética dos espermatozoides de tambaquis após a criopreservação. Utilizaram-se vinte machos de tambaquis (n= 4 pools), que foram induzidos hormonalmente com extrato hipofisário de carpa, para espermiação. Quatorze horas após a indução, realizou-se a coleta seminal. O sêmen de cada pool foi diluído em Ringer adicionado de 10% de dimetilsulfóxido (DMSO) acrescido ou não de GO (T1: sem acréscimo de GO; T2: com 5% de GO e T3: com 10% de GO). O sêmen tratado foi envasado em palhetas de 0,5 mL, congelado em vapor de nitrogênio líquido (dry shipper-30 min/-153 °C) e posteriormente transferidas para nitrogênio líquido. As palhetas foram descongeladas em banho-maria a 37 °C/30 segundos. A taxa de motilidade (%) e a velocidade curvilinear espermática (µm/s) foram analisadas em sistema computadorizado (CASA). Os dados foram expressos em média ± desvio padrão e foi aplicado o teste de Tukey (P<0,05). Houve uma redução significativa na porcentagem de espermatozoides móveis e velocidade curvilinear após a adição de GO independente da concentração. Logo, a adição de GO ao Ringer + DMSO teve efeito negativo sobre a motilidade do sêmen congelado de tambaqui.

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Frozen-thawed Sperm Motility Characteristics of African Catfish (Clarias gariepinus) by Using Glycerol or DMSO Based Extender

Cited by 5 publications

References 23 publications

Cryopreservation and Its Application in Aquaculture

Cryopreservation and Its Application in Aquaculture

Reproductive Tract Morphometry and Evaluation of Fresh Wild African ‎Catfish (Clarias gariepinus) Milt from Lake Alau, Maiduguri, Nigeria‎

QUALIDADE DO SÊMEN DE TAMBAQUI (Colossoma macropomum) CRIOPRESERVADO EM DIFERENTES CONCENTRAÇÕES DE GEMA DE OVO

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