FT-IR microspectroscopy: a promising method for the rapid identification ofListeriaspecies

Abstract

This work presents a pilot study to investigate the potential of fourier transform infrared (FT-IR) microspectroscopy for rapid identification of Listeria at the species level. Using this technique, FT-IR spectra were acquired from 30 strains from five Listeria species. The FT-IR spectra were analysed using stepwise canonical discriminant analysis and partial least-squares regression in a stepwise identification scheme. The results showed that 93% of all the samples were assigned to the correct species, and that 80% of the Listeria monocytogenes strains were correctly identified. In comparison, 100% of the samples, including the L. monocytogenes samples, were correctly identified using spectra acquired by FT-IR macrospectroscopy. The results show that FT-IR microspectroscopy has potential as a rapid screening method for Listeria, which is especially valuable for the food industry.

Can Near-infrared Spectroscopy Detect and Differentiate Implant-associated Biofilms?

“…In a proposed translational application we hope to identify not only the presence or absence of bacteria but also to identify a particular strain thus increasing the importance of improving Type II error. Previous studies have used spectroscopy to analyze biologic specimens [2,10,12,18,21]. Ngo-Thi et al [18] reported the ability of conventional FT-NIR spectroscopy in the MIR region and multivariate data analysis to correctly classify different nonbiofilm-associated bacteria in groups.…”

“…Reflectance spectroscopy provided a similar spectrum compared with conventional techniques in a nondestructive manner [21]. Janbu et al [10] showed that micro-FT-IR spectroscopy using stepwise canonical discriminant analysis could correctly identify strains of Listeria 93% of the time. Bosch et al [2] characterized Bordetella pertussis using spectroscopy and showed that planktonic bacteria have different spectra from its biofilm formation secondary to the spectroscopic absorbance of the glycocalyx or extracellular polymeric substances.…”

An Overview of the Evolution of Infrared Spectroscopy Applied to Bacterial Typing

“…Species FTIRS, FTIRS-ATR, FTIR microspectroscopy CVA, ANN, SCDA, PLSRDA, PCA [5,[21][22][23][24][25]27,49,50] Lactobacillus spp. Species FTIRS HCA, ANN [31,32,39,40,53] Enterococcus spp.…”

“…It was demonstrated the advantage of using macrosamples instead of a microsample approach. [23] Janbu et al [24] also discriminated five Listeria species with FTIRS and FTIR micro-spectroscopy. Obtained discrimination success rates were approximately 93 and 100%, respectively.…”

Detecting bacteria contamination on medical device surfaces using an integrated fiber-optic mid-infrared spectroscopy sensing method

“…During the last several decades, Fourier transform infrared (FTIR) spectroscopy has been extensively used as an effective tool to identify the chemical and molecular structure of different materials without the use of reagents [5][6][7][8][9][10][11][12][13]. Recently, advanced FTIR such as mid infrared (mid-IR) mercury cadmium telluride (MCT) detectors that provide high sensitivity and fast response time in a broad IR spectral range, have made it possible for the FTIR technique to detect and classify microorganisms in a rapid, reliable, and sensitive manner [5,6,8,[14][15][16].…”