FtMYB6, a Light-Induced SG7 R2R3-MYB Transcription Factor, Promotes Flavonol Biosynthesis in Tartary Buckwheat (<i>Fagopyrum tataricum</i>)

Yao, Panfeng; Huang, Yu‐Wen; Dong, Qixin; Wan, Min; Wang, Anhu; Chen, Yuwei; Li, Chenglei; Wu, Qi; Chen, Hui; Zhao, Huihui

doi:10.1021/acs.jafc.0c03037

Cited by 46 publications

(38 citation statements)

References 47 publications

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“…R2R3-MYB TFs are important regulators of flavonoid biosynthesis. The light-induced transcriptions of flavonoid biosynthesis-related TFs have been observed in various plants, such as Arabidopsis thaliana [ 33 ], Fagopyrum tataricum [ 34 ], and pear [ 35 ]. In our study, top five TFs that have the most interactions with individual flavonoid compounds were MYB12 (TEA009412.1), MYB86 (TEA015433.1), C1 (TEA004608.1), MYB4 (TEA033191.1), and KTN80.4 (TEA033903.1).…”

Section: Discussionmentioning

confidence: 99%

Effects of Light Intensity and Spectral Composition on the Transcriptome Profiles of Leaves in Shade Grown Tea Plants (Camellia sinensis L.) and Regulatory Network of Flavonoid Biosynthesis

Liu

et al. 2021

Molecules

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Black net shade treatment attenuates flavonoid biosynthesis in tea plants, while the effect of light quality is still unclear. We investigated the flavonoid and transcriptome profiles of tea leaves under different light conditions, using black nets with different shade percentages, blue, yellow and red nets to alter the light intensity and light spectral composition in the fields. Flavonol glycosides are more sensitive to light intensity than catechins, with a reduction percentage of total flavonol glycosides up to 79.6% compared with 38.7% of total catechins under shade treatment. A total of 29,292 unigenes were identified, and the KEGG result indicated that flavonoid biosynthesis was regulated by both light intensity and light spectral composition while phytohormone signal transduction was modulated under blue net shade treatment. PAL, CHS, and F3H were transcriptionally downregulated with light intensity. Co-expression analysis showed the expressions of key transcription factors MYB12, MYB86, C1, MYB4, KTN80.4, and light signal perception and signaling genes (UVR8, HY5) had correlations with the contents of certain flavonoids (p < 0.05). The level of abscisic acid in tea leaves was elevated under shade treatment, with a negative correlation with TFG content (p < 0.05). This work provides a potential route of changing light intensity and spectral composition in the field to alter the compositions of flavor substances in tea leaves and regulate plant growth, which is instructive to the production of summer/autumn tea and matcha.

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Section: Discussionmentioning

confidence: 99%

Effects of Light Intensity and Spectral Composition on the Transcriptome Profiles of Leaves in Shade Grown Tea Plants (Camellia sinensis L.) and Regulatory Network of Flavonoid Biosynthesis

Liu

et al. 2021

Molecules

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“…The extraction and measurement of Pas were carried out as previously described in [ 51 ]. High-performance liquid chromatography (HPLC) was performed to determine the rutin content according to the description from Yao [ 52 ].…”

Section: Methodsmentioning

confidence: 99%

Tartary Buckwheat R2R3-MYB Gene FtMYB3 Negatively Regulates Anthocyanin and Proanthocyanin Biosynthesis

Wang

Deng

Bai

et al. 2022

IJMS

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Anthocyanins and proanthocyanidins (PAs) are vital secondary metabolites in Tartary buckwheat because of their antioxidant capacities and radical scavenging functions. It has been demonstrated that R2R3-MYB transcription factors (TFs) are essential regulators of anthocyanin and PA biosynthesis in many plants. However, their regulatory mechanisms in Tartary buckwheat remain to be clarified. Here, we confirmed the role of FtMYB3 in anthocyanin and PA biosynthesis. FtMYB3, which belongs to the subgroup 4 R2R3 family was predominantly expressed in roots. The transcriptional expression of FtMYB3 increased significantly under hormone treatment with SA and MeJA and abiotic stresses including drought, salt, and cold at the seedling stage. Functional analyses showed that FtMYB3 negatively regulated anthocyanin and PA biosynthesis, primarily via downregulating the expression of the DFR, ANS, BAN, and TT13 in transgenic Arabidopsis thaliana, which may depend on the interaction between FtMYB3 and FtbHLH/FtWD40. Altogether, this study reveals that FtMYB3 is a negative regulatory transcription factor for anthocyanin and PA biosynthesis in Tartary buckwheat.

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“…It is widely reported that R2R3-MYB transcription factors play essential roles in the regulation of flavonoid biosynthesis (Xu et al, 2015). In Tartary buckwheat, SG4, SG5, SG6, SG7 subfamily MYBs are responsible for this process (Zhang et al, 2017(Zhang et al, , 2018(Zhang et al, , 2019Li et al, 2019c;Yao et al, 2020;Ding et al, 2021). In order to learn more about the genes in the regulatory network of flavonoid biosynthesis, we collected all DEGs annotated to flavonoid biosynthesis genes and SG4, SG5, SG6, SG7 subfamily MYBs and resulting in 12 flavonoid biosynthesis DEGs and nine MYB DEGs (Figure 4A).…”

Section: Myb Transcription Factor Regulated Flavonoid Biosynthesismentioning

confidence: 99%

“…Proanthocyanidins are catalyzed by dihydroflavonol 4-reductase (DFR), leucoanthocyanidin reductase (LAR), and anthocyanidin reductase (Saito et al, 2013;Chen and Li, 2016;Tohge et al, 2017). Massive flavonoid biosynthesis and regulation are characterized by involving enzyme encoding genes and families of transcription factors, including R2R3-MYB, bHLH, and WD40 proteins, and their interactions (MYB-bHLH-WD40 complex) (Kelemen et al, 2015;Xu et al, 2015;Zhang et al, 2018Zhang et al, , 2019Li et al, 2019c;Sun et al, 2020;Yao et al, 2020;Ding et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Integrated Transcriptomics and Widely Targeted Metabolomics Analyses Provide Insights Into Flavonoid Biosynthesis in the Rhizomes of Golden Buckwheat (Fagopyrum cymosum)

Huang

Wang²,

Tang

et al. 2022

Front. Plant Sci.

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Golden buckwheat (Fagopyrum cymosum) is used in Traditional Chinese Medicine. It has received attention because of the high value of its various medicinal and nutritional metabolites, especially flavonoids (catechin and epicatechin). However, the metabolites and their encoding genes in golden buckwheat have not yet been identified in the global landscape. This study performed transcriptomics and widely targeted metabolomics analyses for the first time on rhizomes of golden buckwheat. As a result, 10,191 differentially expressed genes (DEGs) and 297 differentially regulated metabolites (DRMs) were identified, among which the flavonoid biosynthesis pathway was enriched in both transcriptome and metabolome. The integration analyses of the transcriptome and the metabolome revealed a network related to catechin, in which four metabolites and 14 genes interacted with each other. Subsequently, an SG5 R2R3-MYB transcription factor, named FcMYB1, was identified as a transcriptional activator in catechin biosynthesis, as it was positively correlated to eight flavonoid biosynthesis genes in their expression patterns and was directly bound to the promoters of FcLAR2 and FcF3'H1 by yeast one hybrid analysis. Finally, a flavonoid biosynthesis pathway was proposed in the rhizomes of golden buckwheat, including 13 metabolites, 11 genes encoding 9 enzymes, and 1 MYB transcription factor. The expression of 12 DEGs were validated by qRT-PCR, resulting in a good agreement with the Pearson R ranging from 0.83 to 1. The study provided a comprehensive flavonoid biosynthesis and regulatory network of golden buckwheat.

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FtMYB6, a Light-Induced SG7 R2R3-MYB Transcription Factor, Promotes Flavonol Biosynthesis in Tartary Buckwheat (Fagopyrum tataricum)

Cited by 46 publications

References 47 publications

Effects of Light Intensity and Spectral Composition on the Transcriptome Profiles of Leaves in Shade Grown Tea Plants (Camellia sinensis L.) and Regulatory Network of Flavonoid Biosynthesis

Effects of Light Intensity and Spectral Composition on the Transcriptome Profiles of Leaves in Shade Grown Tea Plants (Camellia sinensis L.) and Regulatory Network of Flavonoid Biosynthesis

Tartary Buckwheat R2R3-MYB Gene FtMYB3 Negatively Regulates Anthocyanin and Proanthocyanin Biosynthesis

Integrated Transcriptomics and Widely Targeted Metabolomics Analyses Provide Insights Into Flavonoid Biosynthesis in the Rhizomes of Golden Buckwheat (Fagopyrum cymosum)

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