Fucoidan promotes mechanosensory hair cell regeneration following amino glycoside-induced cell death

Moon, In Seok; So, Ju Hoon; Jung, Young Mi; Lee, Won Sang; Kim, Eun Young; Choi, Jung Hwa; Kim, Cheol-Hee; Choi, Jae Young

doi:10.1016/j.heares.2011.07.007

Cited by 25 publications

(16 citation statements)

References 33 publications

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“…While the molecular function of phoenix is unknown, phoenix mutants do not regenerate HCs due to decreased proliferation of support cells [61]. Chemical screens for compounds that inhibit or enhance HC regeneration have also been performed using the zebrafish lateral line system [62,63]. However, the compounds identified in these screens only modestly affect regeneration by targeting support cell proliferation.…”

Section: Identifying New Molecules/pathways Important For Hair Cell Rmentioning

confidence: 99%

Insights into sensory hair cell regeneration from the zebrafish lateral line

Kniss

Jiang

Piotrowski

2016

Current Opinion in Genetics & Development

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Section: Identifying New Molecules/pathways Important For Hair Cell Rmentioning

confidence: 99%

Insights into sensory hair cell regeneration from the zebrafish lateral line

Kniss

Jiang

Piotrowski

2016

Current Opinion in Genetics & Development

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“…For example, the transcription factor Atoh1 is sufficient for hair cell specification and Atoh1-GFP constructs have been used as markers of hair cell development or as drivers of hair cell regeneration (Zheng and Gao, 2000; Woods et al, 2004; Jones et al, 2006; Lin et al, 2011; Lewis et al, 2012). In the lateral line system in fishes, GFP has been used as a sensory hair cell marker for a range of studies, including research on hair cell death and regeneration (Moon et al, 2011; Namdaran et al, 2012; Coffin et al, 2013). Studies in larval zebrafish engineered to express cytoplasmic or organelle-specific modified FPs demonstrate sequential calcium-dependent signaling events in toxin-treated sensory hair cells, further demonstrating the power of FP reporters for auditory research (Esterberg et al, 2013, 2014).…”

Section: Introductionmentioning

confidence: 99%

Hearing sensitivity differs between zebrafish lines used in auditory research

et al. 2016

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Zebrafish are increasingly used in auditory studies, in part due to the development of several transgenic lines that express hair cell-specific fluorescent proteins. However, it is largely unknown how transgene expression influences auditory phenotype. We previously observed reduced auditory sensitivity in adult Brn3c:mGFP transgenic zebrafish, which express membrane-bound green fluorescent protein (GFP) in sensory hair cells. Here, we examine the auditory sensitivity of zebrafish from multiple transgenic and background strains. We recorded auditory evoked potentials in adult animals and observed significantly higher auditory thresholds in three lines that express hair cell-specific GFP. There was no obvious correlation between hair cell density and auditory thresholds, suggesting that reduced sensitivity was not due to a reduction in hair cell density. FM1-43 uptake was reduced in Brn3c:mGFP fish but not in other lines, suggesting that a mechanotransduction defect may be responsible for the auditory phenotype in Brn3c animals, but that alternate mechanisms underlie the increased AEP thresholds in other lines. We found reduced prepulse inhibition (a measure of auditory-evoked behavior) in larval Brn3c animals, suggesting that auditory defects develop early in this line. We also found significant differences in auditory sensitivity between adults of different background strains, akin to strain differences observed in mouse models of auditory function. Our results suggest that researchers should exercise caution when selecting an appropriate zebrafish transgenic or background strain for auditory studies.

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“…Second, gene expression analyses detected the activation of numerous genes associated with cell proliferation and cell cycle regulation in supporting cells [68,69] . Third, chemical screening identified chemicals that promoted regeneration through increased supporting cell proliferation [70,71] . It is also reported that in the zebrafish adult inner ear, noiseinduced hair cell ablation also triggers the proliferation of supporting cells [72,73] .…”

Section: Cellular Mechanismsmentioning

confidence: 99%

“…Zebrafish larvae are particularly suitable for chemical screening because large numbers of embryos are easily generated, the small size of the larvae allows the use of multi-well plates, and the simplicity of in vivo staining of lateral line hair cells [96] allows for rapid evaluation of the effects of the compounds. To date, 2 chemical screenings have been conducted using zebrafish larvae to identify small compounds involved in hair cell regeneration [70,71] . In addition, other chemical screens have focused on identifying compounds that protect hair cells against ototoxins, or identifying compounds with potential ototoxicity [97][98][99][100][101][102][103] ( fig.…”

Section: Chemical Screeningmentioning

confidence: 99%

Using Zebrafish to Study Human Deafness and Hearing Regeneration

Varshney¹,

Pei²,

Burgess³

2016

Monographs in Human Genetics

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Since the publication of the first draft of the human genome sequence in 2001, there has been an explosion in the number of genes associated with human genetic diseases, including those involved in human deafness. Clinical studies, genome-wide association studies, and exome resequencing have all added to the ever-expanding candidate list of genes with a role in hearing. Because human genetic data is primarily correlative, this explosion of data has increased the need for more efficient approaches to confirm these candidate genes in a model system. In addition, as our understanding of stem cells and genome editing advances, the potential for restoring hearing through regenerative medicine increases. This review highlights the role zebrafish can play as a model for human deafness, and also its potential role in discovering regenerative medicine therapies to restore lost hearing. © 2016 S. Karger AG, BaselHearing loss is one of the most common quality-oflife pathologies affecting the human population. Approximately 2-3 of every 1,000 newborns have a detectable level of hearing loss in one or both ears, and more than 10% of the American population over 18 years of age are suffering with some level of hearing loss, with a clear progression in hearing loss as the person ages (National Institute on Deafness and Other Communication Disorders, http:// www.nidcd.nih.gov/health/ statistics/Pages/quick. aspx). At age 70 and above, over 50% of the population has measurable hearing loss.Hearing is mediated by a sensory epithelium containing acoustic mechanosensory receptors called hair cells and a second cell type known as supporting cells located in the cochlea of the inner ear [1] . This epithelium converts sound waves into electrical impulses ultimately interpreted as hearing in the brain. Hearing loss can be caused by many factors such as exposure to loud noise (https://www.nidcd.nih.gov/staticresources/ health/hearing/NIDCD-Noise-Induced-HearingLoss.pdf), ototoxic chemicals (aminoglycoside antibiotics, cisplatin) [2] , infection [3] , or head injury [4,5] . Genetic factors are likely to play an important role in disease pathogenesis, and recent work has attempted to identify genes that make individuals more susceptible to progressive hearing loss [6] .Like most fish, zebrafish have two related sensory organs that utilize the mechanosensory hair cell receptors: (1) the inner ear similar to mammalian ears used to detect sound, gravity, and motion, and (2) the lateral line [7] , a fish-and am-

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Fucoidan promotes mechanosensory hair cell regeneration following amino glycoside-induced cell death

Cited by 25 publications

References 33 publications

Insights into sensory hair cell regeneration from the zebrafish lateral line

Insights into sensory hair cell regeneration from the zebrafish lateral line

Hearing sensitivity differs between zebrafish lines used in auditory research

Using Zebrafish to Study Human Deafness and Hearing Regeneration

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