Fucosylated glycoproteomic approach to identify a complement component 9 associated with squamous cell lung cancer (SQLC)

Narayanasamy, Arul; Ahn, Jung‐Mo; Sung, Hye-Jin; Kong, Deok-Hoon; Ha, Kwon‐Soo; Lee, Soo-Youn; Cho, Je‐Yoel

doi:10.1016/j.jprot.2011.07.019

Cited by 42 publications

(40 citation statements)

References 31 publications

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“…The secreted proteins were subjected in-gel trypsin digestion as previously reported (Narayanasamy et al, 2011). Mass spectrometry and data analysis were also performed as previously reported (Narayanasamy et al, 2011).…”

Section: Proteomics Analysis By Lc-ms/msmentioning

confidence: 99%

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The Role of Lysyl Oxidase-like 2 in the Odontogenic Differentiation of Human Dental Pulp Stem Cells

Kim

Lee²,

Park

et al. 2013

Molecules and Cells

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Adult human dental pulp stem cells (hDPSCs) are a unique population of precursor cells those are isolated from postnatal dental pulp and have the ability to differentiate into a variety of cell types utilized for the formation of a reparative dentin-like complex. Using LC-MS/MS proteomics approaches, we identified the proteins secreted from the differentiating hDPSCs in mineralization media. Lysyl oxidase-like 2 (LOXL2) was identified as a protein that was down-regulated in the hDPSCs that differentiate into odontoblast-like cells. The role of LOXL2 has not been studied in dental pulp stem cells. LOXL2 mRNA levels were reduced in differentiating hDPSCs, whereas the levels of other LOX family members including LOX, LOXL1, LOXL3, and LOXL4, are increased. The protein expression and secretion levels of LOXL2 were also decreased during odontogenic differentiation. Recombinant LOXL2 protein treatment to hDPSCs resulted in a dose-dependent decrease in the early differentiation and the mineralization accompanying with the lower levels of odontogenic markers such as DSPP, DMP-1 and ALP. These results suggest that LOXL2 has a negative effect on the differentiation of hDPSCs and blocking LOXL2 can promote the hDPSC differentiation to odontoblasts.

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Section: Proteomics Analysis By Lc-ms/msmentioning

confidence: 99%

“…Mass spectrometry and data analysis were also performed as previously reported (Narayanasamy et al, 2011). Briefly, LC-MS/MS analysis was done in Thermo Finnigan's ProteomeX workstation LTQ linear ion trap MS (Thermo Electron, USA) equipped with NSI sources (USA).…”

Section: Proteomics Analysis By Lc-ms/msmentioning

confidence: 99%

The Role of Lysyl Oxidase-like 2 in the Odontogenic Differentiation of Human Dental Pulp Stem Cells

Kim

Lee²,

Park

et al. 2013

Molecules and Cells

Self Cite

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“…30 Briefly, 100 μL of monoclonal antibodies were added to each well of a 96-well ELISA plate and incubated at 37°C for 1 h. The antibodies coated on the plates were oxidized by 200 mM NaIO 4 at 4°C for 5 h and derivatized with 1 mM MPBH and 1 mM Cys-Gly overnight. The plate was then blocked with 3% BSA in PBST (0.1% Tween-20 in PBS) for 1 h. Serum samples were diluted 50-fold with 0.1% Brij in PBST.…”

Section: Mass Spectrometrymentioning

confidence: 99%

Markers of Ovarian Cancer Using a Glycoprotein/Antibody Array

Lubman¹,

Buckanovich²

2013

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“…In a previous study, the high-abundance proteins in the pooled sera were depleted through an immunoaffinity method, and then the sera were subjected to sodium dodecyl sulfonate-polyacrylamide gel electrophoresis (SDS-PAGE), and finally analyzed by LC-ESI-MS/MS. The results showed that C9 protein could serve as a biomarker for squamous cell lung cancer (Narayanasamy et al, 2011). Another proteomic technique of the surface-enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) is increasingly being used to identify the biomarkers in serum indicative of various cancers, such as prostate, ovarian, breast and liver cancers (Adam et al, 2002;Li, Zhang, Rosenzweig, Wang, & Chan, 2002;Petricoin et al, 2002;Schwegler et al, 2005).…”

Section: Introductionmentioning

confidence: 98%

Volunteer study and serum protein profiling to understand inflammatory response induced by Satsuma mandarin

Deng

2015

Food Research International

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Fucosylated glycoproteomic approach to identify a complement component 9 associated with squamous cell lung cancer (SQLC)

Cited by 42 publications

References 31 publications

The Role of Lysyl Oxidase-like 2 in the Odontogenic Differentiation of Human Dental Pulp Stem Cells

The Role of Lysyl Oxidase-like 2 in the Odontogenic Differentiation of Human Dental Pulp Stem Cells

Markers of Ovarian Cancer Using a Glycoprotein/Antibody Array

Volunteer study and serum protein profiling to understand inflammatory response induced by Satsuma mandarin

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