Full characterization of the integrative and conjugative element carrying the metallo-β-lactamaseblaSPM-1and bicyclomycinbcr1resistance genes found in the pandemicPseudomonas aeruginosaclone SP/ST277 : Figure 1.

Fonseca, Érica L.; Abanto, Michel; Encinas, Fernando; Vicente, Ana Carolina Paulo

doi:10.1093/jac/dkv152

Cited by 40 publications

(38 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The isolates PA1088, PA7790, PA11803, and PA12117 showed a duplication of a 4.2 Kb flanking sequence with two directly oriented copies of a region carrying one gene coding a hypothetical protein, one traR , one bcr1 , and one virD2 genes. This context is similar to that described for ICE Tn4371 6061 (Fonseca et al, 2015), except that the transcription direction was inversely oriented since PAGI-15 is located in a genomic region that suffered a major chromosomal inversion. In the remaining two SPM-1-producing P. aeruginosa , PA3448, and PA8281, we observed a duplication of a region measuring approximately 10 Kb possibly caused by recombination of the directly oriented repeats aforementioned, between which bla SPM−1 was inserted, resulting in one additional copy of this gene (Wozniak and Waldor, 2010; Reams et al, 2012).…”

Section: Resultssupporting

confidence: 70%

Intraclonal Genome Stability of the Metallo-β-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an Endemic Clone Disseminated in Brazilian Hospitals

et al. 2016

View full text Add to dashboard Cite

Carbapenems represent the mainstay therapy for the treatment of serious P. aeruginosa infections. However, the emergence of carbapenem resistance has jeopardized the clinical use of this important class of compounds. The production of SPM-1 metallo-β-lactamase has been the most common mechanism of carbapenem resistance identified in P. aeruginosa isolated from Brazilian medical centers. Interestingly, a single SPM-1-producing P. aeruginosa clone belonging to the ST277 has been widely spread within the Brazilian territory. In the current study, we performed a next-generation sequencing of six SPM-1-producing P. aeruginosa ST277 isolates. The core genome contains 5899 coding genes relative to the reference strain P. aeruginosa PAO1. A total of 26 genomic islands were detected in these isolates. We identified remarkable elements inside these genomic islands, such as copies of the blaSPM−1 gene conferring resistance to carbapenems and a type I-C CRISPR-Cas system, which is involved in protection of the chromosome against foreign DNA. In addition, we identified single nucleotide polymorphisms causing amino acid changes in antimicrobial resistance and virulence-related genes. Together, these factors could contribute to the marked resistance and persistence of the SPM-1-producing P. aeruginosa ST277 clone. A comparison of the SPM-1-producing P. aeruginosa ST277 genomes showed that their core genome has a high level nucleotide similarity and synteny conservation. The variability observed was mainly due to acquisition of genomic islands carrying several antibiotic resistance genes.

show abstract

Section: Resultssupporting

confidence: 70%

Intraclonal Genome Stability of the Metallo-β-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an Endemic Clone Disseminated in Brazilian Hospitals

et al. 2016

View full text Add to dashboard Cite

show abstract

“…In addition, a Tn 4371 -like element from P. aeruginosa genomes has recently been characterized to carry bla SPM-1 and bcr1 , suggesting that the Tn 4371 family ICE is an important source of carbapenem resistance in P. aeruginosa 36 . In the current work, we identified a novel ICE Tn 4371 6385 from PASGNDM genomes isolated from clinical sources.…”

Section: Discussionmentioning

confidence: 99%

Acquisition of resistance to carbapenem and macrolide-mediated quorum sensing inhibition by Pseudomonas aeruginosa via ICETn43716385

et al. 2018

View full text Add to dashboard Cite

Pseudomonas aeruginosa can cause life-threatening infections in immunocompromised patients. The first-line agents to treat P. aeruginosa infections are carbapenems. However, the emergence of carbapenem-resistant P. aeruginosa strains greatly compromised the effectiveness of carbapenem treatment, which makes the surveillance on their spreading and transmission important. Here we characterized the full-length genomes of two carbapenem-resistant P. aeruginosa clinical isolates that are capable of producing New Delhi metallo-β-lactamase-1 (NDM-1). We show that blaNDM-1 is carried by a novel integrative and conjugative element (ICE) ICETn43716385, which also carries the macrolide resistance gene msr(E) and the florfenicol resistance gene floR. By exogenously expressing msr(E) in P. aeruginosa laboratory strains, we show that Msr(E) can abolish azithromycin-mediated quorum sensing inhibition in vitro and anti-Pseudomonas effect in vivo. We conclude that ICEs are important in transmitting carbapenem resistance, and that anti-virulence treatment of P. aeruginosa infections using sub-inhibitory concentrations of macrolides can be challenged by horizontal gene transfer.

show abstract

“…The bla NDM-1 was flanked by two IS CR24 -like transposases. bla SPM-1 waslinked to ICE Tn 4371 6061, a recently described ICE [64]. Again, the CEG was located within an IS CR4 -like composite transposon.…”

Section: Resultsmentioning

confidence: 97%

Carbapenemases on the move: it’s good to be on ICE

Botelho

Roberts

León‐Sampedro

et al. 2018

Preprint

View full text Add to dashboard Cite

22 37 38 39 40 41 42 43 44 45 Among the non-fermenting Gram-negative bacteria, the Pseudomonas genus is the one with 46 the highest number of species [1, 2]. Pseudomonas aeruginosa, an opportunistic human 47 pathogen associated with an ever-widening array of life-threatening acute and chronic 48 infections, is the most clinically relevant species within this genus [3-5]. P. aeruginosa is one 49 of the CDC "ESKAPE" pathogens -Enterococcus faecium, Staphylococcus aureus, Klebsiella 50 pneumoniae, Acinetobacter baumannii, P. aeruginosa and Enterobacter species -, 51 emphasizing its impact on hospital infections and the ability of this microorganism to "escape" 52 the activity of antibacterial drugs [6]. P. aeruginosa can develop resistance to a wide range of 53 antibiotics due to a combination of intrinsic, adaptive, and acquired resistance mechanisms, 54 such as the reduction of its outer membrane permeability, over-expression of constitutive or 55 inducible efflux pumps, overproduction of AmpC cephalosporinase, and the acquisition of 56 antibiotic resistance genes (ARGs) through horizontal gene transfer (HGT) [4, 7, 8]. P. 57aeruginosa has a non-clonal population structure, punctuated by specific sequence types 58 (STs) that are globally disseminated and frequently linked to the dissemination of ARGs [4, 9]. 59 These STs have been designated as high-risk clones, of which major examples are ST111, 60 ST175, ST235 and ST244. 61

show abstract

Full characterization of the integrative and conjugative element carrying the metallo-β-lactamasebla_SPM-1and bicyclomycinbcr1resistance genes found in the pandemicPseudomonas aeruginosaclone SP/ST277 : Figure 1.

Abstract: The presence of bla SPM-1 in a Tn4371-ICE with intact integration/conjugation modules demonstrated that, besides gene dispersion by clonal expansion of the pandemic SP/ST277 lineage, bla SPM-1 may be spread through ICE conjugation.

Cited by 40 publications

References 19 publications

Intraclonal Genome Stability of the Metallo-β-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an Endemic Clone Disseminated in Brazilian Hospitals

Intraclonal Genome Stability of the Metallo-β-lactamase SPM-1-producing Pseudomonas aeruginosa ST277, an Endemic Clone Disseminated in Brazilian Hospitals

Acquisition of resistance to carbapenem and macrolide-mediated quorum sensing inhibition by Pseudomonas aeruginosa via ICETn43716385

Carbapenemases on the move: it’s good to be on ICE

Contact Info

Product

Resources

About