Full genome analysis of swine genotype 3 hepatitis E virus isolated from eastern China

Shuai, Jiangbing; Li, Lu-Huan; Li, Aiyun; He, Yanan; Zhang, Xiaofeng

doi:10.1631/jzus.b1600419

Cited by 5 publications

(4 citation statements)

References 17 publications

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“…In the most recent two decades, HEV4 is the main genotype causing human infection in China [ 33 ]. However, several studies reported isolated of HEV3 strains in swine and hepatitis E patients in many provinces in China [ 34 , 35 , 36 ]. One recent study conducted in Shanghai, reported that 1.2% of the local hepatitis E cases were caused by HEV3 [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Hepatitis E Virus Infection among Laboratory Rabbits in China

Wang

Liang

et al. 2021

Pathogens

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Hepatitis E virus (HEV) is zoonotic and the leading cause of acute viral hepatitis worldwide. Rabbit HEV can infect humans and is prevalent globally. It is reported that laboratory rabbits are also naturally infected with HEV. Therefore, it is important to investigate in a large scale the prevalence of HEV in laboratory rabbits. Serum samples were collected from 649 laboratory rabbits of 13 different commercial vendors in Beijing, China, from 2017 to 2019, and anti-HEV and HEV antigen (Ag) were tested. Fecal samples were collected from 50 laboratory rabbits from one of the vendors for HEV RNA detection. Six laboratory rabbits with natural HEV infection were euthanized and their liver, kidney, bile and urine samples were collected for HEV RNA quantification. Liver tissues were subjected to histopathology analysis. The overall positive rates of anti-HEV antibodies and HEV-Ag are 2.6% (15/588) and 7.9% (51/649), respectively. HEV RNA was detected in 12.0% (6/50) of the rabbits. High viral load of HEV RNA was detected in liver and bile samples. Liver inflammation was observed. HEV is circulating in laboratory rabbit population in China. Strict screening is crucial to ensure experimental accuracy and prevent zoonotic transmission to research personnel.

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Section: Discussionmentioning

confidence: 99%

Prevalence of Hepatitis E Virus Infection among Laboratory Rabbits in China

Wang

Liang

et al. 2021

Pathogens

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“…HEV genotype 3 and 4 HEV have become the predominant HEV genotypes in China (Shuai et al, 2017;Zhang et al, 2009). All eight HEV strains isolated from buffaloes in Guangxi Province belong to genotype 4 HEV.…”

Section: Discussionmentioning

confidence: 99%

Hepatitis E virus infection in buffaloes in South China

Wei

Jia

et al. 2020

Arq. Bras. Med. Vet. Zootec.

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Hepatitis E virus (HEV) infection is an important global public health issue. HEV infections are recognized as a zoonotic disease. Swine are believed to be the main reservoir of HEV. Recently, yaks, cows, and yellow cattle have been reported as new reservoirs of HEV. However, whether other species of cattle and buffaloes are sensitive to HEV infection is unknown. To investigate the prevalence of HEV infection in buffaloes, enzyme-linked immunosorbent assay (ELISA) and reverse transcription-nested polymerase chain reaction (RT-nPCR) were performed. Only one buffalo was positive to anti-HEV IgM antibody (1/106, 0.94%), and none were positive for anti-HEV IgG antibody. To our surprise, five serum (5/106, 4.72%) and three milk samples (3/40, 7.50%) from buffaloes were positive to HEV RNA. All strains of HEV isolated from buffaloes belong to genotype 4. Results indicate that buffaloes may be a new reservoir of HEV.

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“…Hepatitis E virus falls into the genus Orthohepevirus of the family Hepeviridae. The genus Orthohepevirus is composed of 4 species (Orthohepevirus A to Orthohepevirus D), and Orthohepevirus A comprises 7 genotypes (HEV-1 to HEV-7) (Johne et al, 2014;Smith et al, 2014;Krzowska-Firych et al, 2018). Among the 7 genotypes, HEV-1 and HEV-2 can only infect people and the infection route is mainly associated with drinking water contaminated by HEV (Purcell and Emerson, 2008;Pérez-Gracia et al, 2014;King et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…HEV isolates from rabbits have a genome of approximately 7.2 kb, containing 3 open reading frames, genetically close to HEV-3 (Cossaboom et al, 2012;Smith et al, 2014). Rabbit HEV can not only cause mild hepatitis in rabbits (Liu et al, 2013), but can also replicate in A549 and PLC/PRF/5 cells of human origin (Jirintai et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Molecular features of hepatitis E virus from farmed rabbits in Shandong province, China

2018

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<p>This study was undertaken to investigate the genetic variability of hepatitis E virus (HEV) from farmed rabbits in Shandong province, China. A total of 50 fresh faecal samples from 5 rabbit farms were collected and subjected to reverse transcription and nested polymerase chain reaction (RT-nPCR) for a fragment sequence of HEV capsid gene. The results demonstrated that HEV RNA was observed in 6 faecal samples (6/50, 12.0%). In addition, the result of phylogenetic analysis showed that the 6 HEV isolates were classified into HEV-3 genotype with other rabbit HEV isolates from other countries, and shared 85.2-87.2%, 81.5-83.1%, and 77.0-78.6% nucleotide similarities with rabbit HEV isolates from Korea, the United States and France, respectively. To sum up, the HEV isolated in this study from farmed rabbits belongs to the HEV-3 genotype, and the zoonotic ability and pathogenesis of the rabbit HEV merit further study due to the fact that HEV-3 genotype has the potential to trigger zoonotic infections.</p>

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Full genome analysis of swine genotype 3 hepatitis E virus isolated from eastern China

Cited by 5 publications

References 17 publications

Prevalence of Hepatitis E Virus Infection among Laboratory Rabbits in China

Prevalence of Hepatitis E Virus Infection among Laboratory Rabbits in China

Hepatitis E virus infection in buffaloes in South China

Molecular features of hepatitis E virus from farmed rabbits in Shandong province, China

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