Full survival of paraquat-exposed rats after treatment with sodium salicylate☆

Dinis-Oliveira, Ricardo Jorge; Sousa, Carla; Remião, Fernando; Duarte, José Alberto; Navarro, Amparo Sánchez; Bastos, Maria de Lourdes; Carvalho, Félix

doi:10.1016/j.freeradbiomed.2006.12.031

Cited by 87 publications

(64 citation statements)

References 42 publications

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“…These cells together with lung epithelial and endothelial cells can produce a feedback circle and activate alveolar and interstitial macrophages. MPO is located within the primary azurophil granules of polymorphonuclear neutrophils and its activity indirectly reflects polymorphonuclear neutrophil infiltration through the organs during the inflammatory response (21,22). Inflammatory cells secrete various kinds of cytokines such as TNF-α, MCP-1 and TGF-β1, which may amplify or attenuate inflammatory reactions and lead to pulmonary fibrosis (6,23).…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Ambroxol against Paraquat-induced Pulmonary Fibrosis in Rats

2011

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Objective To evaluate the possible therapeutic effect of ambroxol on pulmonary fibrosis induced by paraquat. Methods Adult male Sprague-Dawley rats (n =144, 200-250 g) were divided into four groups (Control, Ambroxol, Paraquat, and Paraquat+Ambroxol group) and sacrificed on day 1, 3, 5, 7, 14 and 28. Several significant oxidant stress markers (MDA, SOD and GSH-PX), MPO activity, cytokines (TNF-α, MCP-1, TGF-β1, MMP-2 and TIMP-1), total inflammatory cell count, hydroxyproline content, collagen I and III mRNA were analyzed. Results In Paraquat group, the MDA, MPO activity, hydroxyproline contents, the mRNA expression of TNF-α, MCP-1, TGF-β1, MMP-2, TIMP-1, collagen I, collagen III and the number of total inflammatory cells were up-regulated in lung tissue, but SOD and GSH-PX activity were down-regulated in lung tissue compared with Control group (p<0.05). In paraquat+ambroxol group, the MDA, MPO activity, hydroxyproline content, the mRNA expression of TNF-α, MCP-1, TGF-β1, MMP-2, TIMP-1 collagen I, collagen III and the number of total inflammatory cells were significantly decreased, while the SOD and GSH-PX activities in lung tissue were increased compared with Paraquat group (p<0.05). Histological examination of paraquat-treated rats showed lung injury with interstitial edema and widespread inflammatory cell infiltration in the alveolar space and septum, as well as pulmonary fibrosis. Ambroxol could markedly reduce such damage in lung tissue and prevent pulmonary fibrosis. Conclusion The results of this study indicated that ambroxol could reduce lung damage and prevent pulmonary fibrosis induced by paraquat.

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Section: Discussionmentioning

confidence: 99%

Protective Effect of Ambroxol against Paraquat-induced Pulmonary Fibrosis in Rats

2011

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“…Recently, our research group showed an additional application for this drug, by demonstrating that sodium salicylate (NaSAL) could represent a powerful antidote to be used against paraquat (PQ) poisonings (Dinis-Oliveira et al, 2007a). In that study, the remarkable healing effects obtained by the administration of NaSAL, asserted by histological, functional, and survival analysis, were shown to be associated to an effective inhibition of pro-inflammatory factors such as NF-B, to the scavenging of reactive oxygen species (ROS), and also through the inhibition of myeloperoxidase activity and inhibition of platelet aggregation.…”

Section: Introductionmentioning

confidence: 99%

Reactivity of paraquat with sodium salicylate: Formation of stable complexes

et al. 2008

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“…Furthermore, salicylic acid inhibits cyclooxygenase (EC 1.14.99.1), a key enzyme in the pathway of prostaglandin synthesis. Products of this pathway have many physiological functions in both normal and disease conditions [24,25] and are known to influence inflammatory processes associated with oxidative stress [26][27][28]. Thus the biochemical actions of salicylate itself may perturb the hROS results.…”

Section: Salicylatementioning

confidence: 99%