Fullerenol C 60 (OH) 36 at relatively high concentrations impairs hippocampal theta oscillations (in vivo and in vitro) and triggers epilepsy (in vitro) – A dose response study

Kazmierska-Grebowska, Paulina; Kobrzycka, Anna; Bocian, Renata; Kowalczyk, Tomasz; Krokosz, Anita; Grebowski, Jacek

doi:10.1016/j.yexmp.2018.06.002

Cited by 4 publications

(4 citation statements)

References 62 publications

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“…Fullerols C 60 (OH) 24 (FNP) are water soluble fullerene C 60 daughter products with poorly defined environmental reactivity, distinctive material properties, and biological activities, which have gained great attention today. There are several available toxicity studies of FNP 1 – 5 ; however, these studies were not carried out on fungal species. For example, Vávrová et al .…”

Section: Introductionmentioning

confidence: 99%

“…Kazmierska-Grebowska et al . 5 , however, reported that fullerol C 60 (OH) 36 exhibited dose-dependent effect by impairing hippocampal theta oscillations ( in vivo and in vitro ) and triggering epilepsy ( in vitro ) at relatively high concentrations (60 μM and 80 μM in vitro ; 0.2 μg μL −1 in vivo ) but exerting no apparent effects at lower concentrations (20 μM, 40 μM in vitro ; 0.05 μg μL −1 , 0.1 μg μL −1 , 0.15 μg μL −1 in vivo ).…”

Section: Introductionmentioning

confidence: 99%

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Fullerol C60(OH)24 nanoparticles modulate aflatoxin B1 biosynthesis in Aspergillus flavus

Kovač

Borišev

Crevar

et al. 2018

Sci Rep

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The water soluble fullerene C60 daughter product - fullerols C60(OH)24 (FNP) possesses a great potential of modifying secondary metabolites biosynthesis. In order to clarify the extent of interaction, the impact of FNP (10, 100 and 1000 ng mL−1) on aflatoxin production and the available precursors of biosynthesis pathway from Aspergillus flavus NRRL 3251 was determined, in both the mycelia and yeast extract sucrose (YES) medium, during a 168-hour growth period at 29 °C in the dark. The FNP of 8 nm in diameter, and with a zeta potential of −33 mV affected mycelial growth at 1000 ng mL−1 while conidia production was slightly affected at 10 ng mL−1. The FNP effect on aflatoxin and it biosynthetic precursors was concentration dependent and alteration of the sterigmatocystin (ST) export from the cell was observed. Most of the monitored aflatoxin precursors, except norsolorinic acid, were detected in both mycelia and YES medium. However, observed precursor concentrations were much higher in mycelia, with exception of ST. The study shows the loss of FNP antioxidative effect after 120 hours of growth, and strong concentration dependent aflatoxigenic effect after that time. Thus, this data is relevant to guide future considerations on FNP-fungal interactions in the environments and on risk assessment.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fullerol C60(OH)24 nanoparticles modulate aflatoxin B1 biosynthesis in Aspergillus flavus

Kovač

Borišev

Crevar

et al. 2018

Sci Rep

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“…In our experiments, we tested the enzyme activity in erythrocytes subjected to oxidative stress induced by prolonged incubation, in the presence of C 60 (OH) 36 . Fullerenes are an important class of nanomaterials with a wide potential applicability in biomedical sciences [8], and their water-soluble derivatives (fullerenols) are promising candidates for use as antioxidants in biological systems [10][11][12][13][14][15][16][17]. C 60 (OH) 36 prevents ROS cell damage, but also can scavenge NO [13,20,21,38].…”

Section: Discussionmentioning

confidence: 99%

“…Fullerenes and their derivatives are considered effective free radical scavengers, including ROS, based on the large number of conjugated double π bonds [ 8 ]. Their water-soluble derivatives C 60 (OH) n (fullerenols) have recently been the subject of numerous investigations regarding the radioprotective and antioxidant properties in biological and biomimetic systems [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ]. Covalently attached hydroxyl groups play a crucial role for the localization of fullerenol and its accessibility for ROS, whereas the mechanism of antioxidant action of C 60 (OH) n depends on the nature of ROS [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Fullerenol C60(OH)36 on the Antioxidant Defense System in Erythrocytes

Grebowski

Kazmierska-Grebowska

Cichoń

et al. 2021

IJMS

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Background: Fullerenols (water-soluble derivatives of fullerenes), such as C60(OH)36, are biocompatible molecules with a high ability to scavenge reactive oxygen species (ROS), but the mechanism of their antioxidant action and cooperation with endogenous redox machinery remains unrecognized. Fullerenols rapidly distribute through blood cells; therefore, we investigated the effect of C60(OH)36 on the antioxidant defense system in erythrocytes during their prolonged incubation. Methods: Human erythrocytes were treated with fullerenol at concentrations of 50–150 µg/mL, incubated for 3 and 48 h at 37 °C, and then hemolyzed. The level of oxidative stress was determined by examining the level of thiol groups, the activity of antioxidant enzymes (catalase, glutathione peroxidase, glutathione reductase, and glutathione transferase), and by measuring erythrocyte microviscosity. Results: The level of thiol groups in stored erythrocytes decreased; however, in the presence of higher concentrations of C60(OH)36 (100 and 150 µg/mL), the level of -SH groups increased compared to the control. Extending the incubation to 48 h caused a decrease in antioxidant enzyme activity, but the addition of fullerenol, especially at higher concentrations (100–150 µg/mL), increased its activity. We observed that C60(OH)36 had no effect on the microviscosity of the interior of the erythrocytes. Conclusions: In conclusion, our results indicated that water-soluble C60(OH)36 has antioxidant potential and efficiently supports the enzymatic antioxidant system within the cell. These effects are probably related to the direct interaction of C60(OH)36 with the enzyme that causes its structural changes.

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Classification of the Methods for the Synthesis of Polyhydroxylated Fullerenes. Part 2. One‑Step and Multi‑Step Procedures

Ignatev,

Muller,

Pasynkov

et al. 2024

Chem Technol Fuels Oils

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Fullerenol C 60 (OH) 36 at relatively high concentrations impairs hippocampal theta oscillations (in vivo and in vitro) and triggers epilepsy (in vitro) – A dose response study

Cited by 4 publications

References 62 publications

Fullerol C60(OH)24 nanoparticles modulate aflatoxin B1 biosynthesis in Aspergillus flavus

Fullerol C60(OH)24 nanoparticles modulate aflatoxin B1 biosynthesis in Aspergillus flavus

The Effect of Fullerenol C60(OH)36 on the Antioxidant Defense System in Erythrocytes

Classification of the Methods for the Synthesis of Polyhydroxylated Fullerenes. Part 2. One‑Step and Multi‑Step Procedures

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