Fullerenol C60(OH)24 prevents doxorubicin-induced acute cardiotoxicity in rats

Abstract

Results obtained in vitro suggested that fullerenol's antiproliferative properties and protective effects against doxorubicin (DOX) cytotoxicity are mediated by antioxidative and hydroxyl radical scavenger activity. The aim of this study was to examine the influence of fullerenol on acute cardiotoxicity after the administration of a single high dose of DOX in vivo. The experiment was performed on male Wistar rats randomly divided into five groups, each containing eight individuals, that were treated as follows: I) 0.9% NaCl, II) 10 mg/kg DOX, III) 50 mg/kg fullerenol 30 min before 10 mg/kg DOX, IV) 100 mg/kg fullerenol 30 min before 10 mg/kg DOX, and V) 100 mg/kg fullerenol. A functional, biochemical, hematological, and pathomorphological examination of the heart as well as an evaluation of oxidative stress parameters was conducted on days 2 and 14 after DOX administration. The function of the heart was investigated by monitoring heart contractility after the adrenaline infusion. Fullerenol, applied alone, did not alter basal values of investigated animals. Both doses of fullerenol, used as a pretreatment, did not alter the basal parameters of the animals. The 100 mg/kg dose of fullerenol showed better protection. Considering the mechanisms of DOX toxicity, fullerenol likely exerts its protective role as a free radical sponge and/or by removing free iron through the formation of a fullerenol-iron complex. Our results suggest that fullerenol might be a potential cardioprotective agent in DOX-treated individuals.

Antioxidative nanofullerol prevents intervertebral disk degeneration

“…Among numerous reported fullerene derivatives, nanofullerol is the most extensively studied, and has been implicated as a powerful antioxidant in the biomedical field. [48][49][50][51][52][53][54][55][56] Therefore, fullerol was selected in our present study.…”

“…These results are in accordance with those previously reported that nanofullerol provided protection against cell death induced by ROS in a variety of cells and tissues, including keratinocytes 62 and endothelial, 63 heart, liver, kidney, testes, and lung tissues. 50,51,64,65 It is well known that GAGs are the dominant part of the proteoglycan aggrecan, and aggrecan is a major component of the extracellular matrix in both chondrocytes and NP cells. 66,67 Yudoh et al reported that a water-soluble fullerene prevents matrix degradation in human chondrocytes under catabolic stress induced by H 2 O 2 and IL-1β.…”

Antioxidative fullerol promotes osteogenesis of human adipose-derived stem cells

“…31 Our present data showed that fullerol served as an antioxidant in human ADSCs without cytotoxicity at doses up to 10 µM (Figures 1 and 2), and this is in agreement with a majority of previous reports with regards to other tissues and cell types. [17][18][19]28,[32][33][34][35][36][37][38][39] It has been shown that ROS inhibit osteogenesis and bone formation. Mody et al 40 reported that three oxidants, hydrogen peroxide (H 2 O 2 ), xanthine, and xanthine oxidase, and minimally oxidized low-density lipoproteins inhibited osteoblastic differentiation of MC3T3-E1 mouse bone preosteoblast cell line and M2-10B4 mouse marrow stromal cell line, while increasing intracellular oxidative stress.…”

Biological applications of hydrophilic C60 derivatives (hC60s)− a structural perspective

“…Lactate dehydrogenase (LDH) and α-hydroxybutyrate dehydrogenase (α-HBDH) in cardiomyocytes were marks to evaluate the tissue injuries. Both of them being elevated by DOX decreased by C60(OH)24 [35]. Except the cardioprotection, C60(OH)24 prevented the oxidative injury from the lung and kidney as well.…”

“…In addition, 100 mg/kg C60(OH)24 maintained the level of antioxidative enzymes superoxide in cardiomyocytes which would be enhanced by 8 mg/kg DOX. The enzymes contained SOD, catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) [35]. Lactate dehydrogenase (LDH) and α-hydroxybutyrate dehydrogenase (α-HBDH) in cardiomyocytes were marks to evaluate the tissue injuries.…”