Fully automated hot embossing processes utilizing high resolution working stamps

Glinsner, T.; Veres, Teodor; Kreindl, Gerald; Roy, Emmanuel; Morton, Keith; Wieser, T.; Thanner, C.; Treiblmayr, D.; Miller, R. A.; Lindner, Paul

doi:10.1016/j.mee.2009.11.098

Cited by 14 publications

(4 citation statements)

References 6 publications

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“…We fabricated a mold consisting of the PR chambers, channel, and frits and a mold for the substrate containing alignment grooves for the input/output glass capillaries on Si wafers. The SU-8 molds were then transferred to a soft polymeric mold (MD700 working stamps, Solvay) via an intermediate PDMS transfer mold. PDMS replicas of the SU-8 are fabricated as described by others: PDMS and curing agent (Sylgard 184, Dow Corning) were mixed in a 10:1 mass ratio, poured onto the SU-8 mold, degassed, and cured at 80 °C for 2 h. The working stamp mold was fabricated by applying MD700 polymer onto the PDMS mold with a photocurable agent and exposed to UV light (40 mJ, 15 min).…”

Section: Methodsmentioning

confidence: 99%

Development of a Multiplexed Microfluidic Proteomic Reactor and Its Application for Studying Protein–Protein Interactions

Tian

Hoa²,

Lambert

et al. 2011

Anal. Chem.

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Mass spectrometry-based proteomics techniques have been very successful for the identification and study of protein–protein interactions. Typically, immunopurification of protein complexes is conducted, followed by protein separation by gel electrophoresis and in-gel protein digestion, and finally, mass spectrometry is performed to identify the interacting partners. However, the manual processing of the samples is time-consuming and error-prone. Here, we developed a polymer-based microfluidic proteomic reactor aimed at the parallel analysis of minute amounts of protein samples obtained from immunoprecipitation. The design of the proteomic reactor allows for the simultaneous processing of multiple samples on the same devices. Each proteomic reactor on the device consists of SCX beads packed and restricted into a 1 cm microchannel by two integrated pillar frits. The device is fabricated using a combination of low-cost hard cyclic olefin copolymer thermoplastic and elastomeric thermoplastic materials (styrene/(ethylene/butylenes)/styrene) using rapid hot-embossing replication techniques with a polymer-based stamp. Three immunopurified protein samples are simultaneously captured, reduced, alkylated, and digested on the device within 2–3 h instead of the days required for the conventional protein–protein interaction studies. The limit of detection of the microfluidic proteomic reactor was shown to be lower than 2 ng of protein. Furthermore, the application of the microfluidic proteomic reactor was demonstrated for the simultaneous processing of the interactome of the histone variant Htz1 in wild-type yeast and in a swr1Δ yeast strain compared to an untagged control using a novel three-channel microfluidic proteomic reactor.

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Section: Methodsmentioning

confidence: 99%

Development of a Multiplexed Microfluidic Proteomic Reactor and Its Application for Studying Protein–Protein Interactions

Tian

Hoa²,

Lambert

et al. 2011

Anal. Chem.

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“…The mold can consist of different materials, such as silicon or nickel coated metals, and substrate material variants can be a variety of polymers and even glass 55. Depending on the substrate material used, and the hot embossing technique, the resolution can be as precise as 50 nm 57. Hot embossing offers an easy and inexpensive method for production, with high throughput and high accuracy 55.…”

Section: Materials and Manufacturing Methodsmentioning

confidence: 99%

Integrating Micro Process Chemistry into an NMR Spectrometer

Schulte-Hermann,

Yang,

Hurtado Rivera

et al. 2023

Chemie Ingenieur Technik

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Miniaturization has proven to be a compelling strategy in many research areas and continuous progress in micro fabrication has contributed to its growing popularity. These advances offer opportunities in analytical characterization technologies. Nuclear magnetic resonance is one such characterization method that has greatly benefited from miniaturization. Many research opportunities remain to be explored, leveraging the advantages of miniaturization in NMR. Here, the benefits of NMR‐miniaturization in the fields of micro process engineering, gas‐based hyperpolarization, and small‐scale bioreactors are reviewed. These applications are discussed in the context of modern micro fabrication approaches and materials, highlighting the most compatible with NMR applications.

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“…The use here of fluorinated polymer working stamp facilitates the demolding process after hot embossing. The working stamp was created by pouring liquid precursor of UV-curable polymer (MD700) mixed with 1% photoinitiator (Darocur ® 1173) onto the Si master [ 32 ] following with flood lamp UV curing at room temperature (Dymax ECE 2000 UV, Torrington, CT, 2 min). The working stamp was used to hot emboss COC substrates (Zeonor 1060 R) under vacuum at 10 kN and 140 °C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Facile Fabrication of Flexible Polymeric Membranes with Micro and Nano Apertures over Large Areas

Hernández-Castro²,

Morton

et al. 2022

Polymers

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Freestanding, flexible and open through-hole polymeric micro- and nanostructured membranes were successfully fabricated over large areas (>16 cm2) via solvent removal of sacrificial scaffolds filled with polymer resin by spontaneous capillary flow. Most of the polymeric membranes were obtained through a rapid UV curing processes via cationic or free radical UV polymerisation. Free standing microstructured membranes were fabricated across a range of curable polymer materials, including: EBECRYL3708 (radical UV polymerisation), CUVR1534 (cationic UV polymerisation) UV lacquer, fluorinated perfluoropolyether urethane methacrylate UV resin (MD700), optical adhesive UV resin with high refractive index (NOA84) and medical adhesive UV resin (1161-M). The present method was also extended to make a thermal set polydimethylsiloxane (PDMS) membranes. The pore sizes for the as-fabricated membranes ranged from 100 µm down to 200 nm and membrane thickness could be varied from 100 µm down to 10 µm. Aspect ratios as high as 16.7 were achieved for the 100 µm thick membranes for pore diameters of approximately 6 µm. Wide-area and uniform, open through-hole 30 µm thick membranes with 15 µm pore size were fabricated over 44 × 44 mm2 areas. As an application example, arrays of Au nanodots and Pd nanodots, as small as 130 nm, were deposited on Si substrates using a nanoaperture polymer through-hole membrane as a stencil.

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Fully automated hot embossing processes utilizing high resolution working stamps

Cited by 14 publications

References 6 publications

Development of a Multiplexed Microfluidic Proteomic Reactor and Its Application for Studying Protein–Protein Interactions

Development of a Multiplexed Microfluidic Proteomic Reactor and Its Application for Studying Protein–Protein Interactions

Integrating Micro Process Chemistry into an NMR Spectrometer

Facile Fabrication of Flexible Polymeric Membranes with Micro and Nano Apertures over Large Areas

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