Fully automated immunoassay system of endocrine disrupting chemicals using monoclonal antibodies chemically conjugated to bacterial magnetic particles

Immunochemical determination of xenobiotics with endocrine disrupting effects

“…Several methodologies have been developed using these available monoclonal antibodies [79,80,81], all of them with the purpose of developing automated systems. The main results are shown in Table 1 [79] to obtain polyclonal antibodies incubation step between the antibody, the analyte, and the labeled Ag (Ag*) is carried out off-line for a short time and then is injected in the flow system.…”

“…Matsunaga et al have developed an automated heterogeneous immunoassay based on the immobilization of the monoclonal antibodies (from Takeda Industries) on bacterial magnetic particles [81] (i.e., magnetite particles synthesized by magnetic bacteria). The magnetic properties of these particles permit the easy separation of the immobilized reagent from the media in different sequential steps.…”

“…Related bisphenolic compounds, other EDCs, surfactants, and fumic acids have been tested and no cross-reactivity has been observed. As in the case of APEs, Matsunaga et al [81] have developed an automated immunoassay based on the immobilization of these monoclonal antibodies on bacterial magnetic particles. The sensitivity of this assay is very good with a detection range of 2.3 ng L -1 to 2.3 mg L -1 , that is, even better than the direct ELISA and conventional chromatographic methods.…”

Magnetic nanoparticle drug delivery systems for targeting tumor

“…Bacterial magnetosomes (BMs) synthesized by magnetotactic bacteria have also been used as carriers for enzymes, nucleic acids and antibodies (Balkwill et al 1980;Ota et al 2003;Matsunaga et al 2003). Their application as targeted drug carriers has been commended due to their unique features, such as paramagnetism, nanoscale, narrow size distribution and membrane-bound form (Grünberg et al 2004).…”

Chemiluminescence immunoassay

“…Enzymes and Abs have been immobilized on BMPs using both bifunctional reagents and glutaraldehyde and have been found to have higher activities than those immobilized onto artificial magnetic particles. Matsunaga et al [79][80][81] developed a CL EIA using Ab-immobilized BMPs for the determination of mouse immunoglobulin G (IgG), E2, alkylphenol ethoxylates (APEs), bisphenol A (BPA) and linear alkylbenzene sulfonates (LASs) in environmental water. In order to obviate the need for immobilization of Ab to BMP by covalent chemical crosslinking, protein A-BMP complexes harvested from transconjugant AMB-1 were subsequently complexed with Abs by specific binding between the Z domain of protein A and the Fc component of IgG to form the Abprotein A-BMP complexes [85].…”