Fully Automated Lab-On-A-Disc Platform for Loop-Mediated Isothermal Amplification Using Micro-Carbon-Activated Cell Lysis

Abstract

Fast and fully automated deoxyribonucleic acid (DNA) amplification methods are of interest in the research on lab-on-a-disc (LOD) platforms because of their full compatibility with the spin-column mechanism using centrifugal force. However, the standard procedures followed in DNA amplification require accurate noncontact temperature control as well as cell lysis at a low temperature to prevent damage to the LOD platform. This requirement makes it challenging to achieve full automation of DNA amplification on an LOD. In this paper, a fully automated LOD capable of performing cell lysis and amplification on a single compact disc of DNA samples is proposed. The proposed system uses micro-carbon to heat DNA samples without damaging the LOD as well as a noncontact heating system and an infrared camera sensor to remotely measure the real temperature of the amplification chamber. Compared with conventional DNA amplification systems, the proposed system has the advantage of full automation of the LOD platform. Experimental results demonstrated that the proposed system offers a stable heating method for DNA amplification and cell lysis.

Customizable Nichrome Wire Heaters for Molecular Diagnostic Applications

“…However, they are inefficient, and is difficult to control the temperature [ 33 ]. Some groups have utilized lasers as heaters since they allow non-contact and local heating by having embedded conductive material such as carbon black or graphite inside the microfluidic chip [ 34 ], demonstrating low energy consumption and high ramping rate [ 35 ]. Nevertheless, high-power lasers and peripheral hardware to control the laser’s power are expensive.…”

Chemical Trends in Sample Preparation for Nucleic Acid Amplification Testing (NAAT): A Review

“…Lysis techniques include chemical, mechanical, and thermal lysis, with chemical lysis approaches covering detergents, chaotropic reagents, enzymes, and others. Other reviews have focused on the suitability of different lysis techniques for various sample types [13][14][15][16][17][18][19], and progress towards the integration of lysis techniques in microfluidic devices can be found elsewhere [12,20,21]. While chemical lysis has traditionally been highly effective, carryover of the reagents at 1-10% can cause a complete or significant inhibition of amplification [22,23].…”