Temperature transducers are frequently employed to keep track of process variables with different kinds of industrial controllers. One of the widely used temperature sensors is Pt100. A novel approach of utilizing an electroacoustic transducer in signal conditioning for Pt100 is proposed in this paper. A “signal conditioner” is a resonance tube filled with air, which is operated in a free resonance mode. The Pt100 wires are connected to one of the leads of the speaker in the resonance tube where the temperature changes, which is related to Pt100 resistance. The resistance affects the amplitude of the standing wave that is detected by an electrolyte microphone. An algorithm for measuring the amplitude of the speaker signal is described, as well as the building and functioning of the electroacoustic resonance tube signal conditioner. The microphone signal is acquired as a voltage using LabVIEW software. A virtual instrument (VI) developed under LabVIEW provides a measure of the voltage using standard VIs. The findings of the experiments reveal a link between the measured amplitude of the standing wave within the tube and the change in Pt100 resistance as the ambient temperature changes. Additionally, the suggested method may interface with any computer system when a sound card is added to it without the need for any extra measuring tools. The maximum nonlinearity error at full-scale deflection (FSD) is estimated at roughly 3.77%, and the experimental results and a regression model are used to assess the relative inaccuracy of the developed signal conditioner. When comparing the proposed approach with well-known approaches for Pt100 signal conditioning, the proposed one has several advantages such as its simplicity of connecting Pt100 to a personal computer directly via the sound card of any personal computer. In addition, there is no need for a reference resistance to perform a temperature measurement using such a signal conditioner.