Fully habituated sugarbeet callus: Under permanent stress?

Dily, Frédérik Le; Billard, J. P.; Huault, C.; Kevers, Claire; Gaspar, Thomas

doi:10.1007/bf02632027

Cited by 9 publications

(3 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…tataricum are in a state of continuous oxidative stress, which is reflected by their higher content of hydrogen peroxide and malonic dialdehyde, low catalase activity and high activity of superoxide dismutase compared to the MC cells. It should be noted that high levels of lipid peroxidation and the malondialdehyde accumulation are typical for a fully habituated non-organogenic sugar beet callus [ 63 ]. Although the TUNEL test has previously been applied in some analyses of DNA damage in callus cells [ 6 , 62 , 64 ], our study is still quite a rare example of this type of application.…”

Section: Resultsmentioning

confidence: 99%

Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn

et al. 2017

View full text Add to dashboard Cite

Long-term cultivated Fagopyrum tataricum (L.) Gaertn. (Tartary buckwheat) morphogenic and non-morphogenic callus lines are interesting systems for gaining a better understanding of the mechanisms that are responsible for the genetic stability and instability of a plant tissue culture. In this work, we used histological sections and transmission electron microscopy to identify and describe the morphology of the nuclei of all of the analysed callus lines. We demonstrated that the embryogenic callus cells had prominent round nuclei that did not contain heterochromatin clumps in contrast to the non-morphogenic callus lines, in which we found nuclei that had multiple lobes. Flow cytometry analysis revealed significant differences in the relative DNA content between the analysed calli. All of the analysed morphogenic callus lines had peaks from 2C to 8C as compared to the non-morphogenic callus lines, whose peaks did not reflect any regular DNA content and exceeded 8C and 16C for the line 6p1 and 16C and 32C for the callus line 10p2A. The results showed that non-morphogenic calli are of an aneuploid nature. The TUNEL test enabled us to visualise the nuclei that had DNA fragmentation in both the morphogenic and non-morphogenic lines. We revealed significantly higher frequencies of positively labelled nuclei in the non-morphogenic lines than in the morphogenic lines. In the case of the morphogenic lines, the highest observed frequency of TUNEL-positive nuclei was 7.7% for lines 2–3. In the non-morphogenic calli, the highest level of DNA damage (68.5%) was revealed in line 6p1. These results clearly indicate greater genome stability in the morphogenic lines.

show abstract

Section: Resultsmentioning

confidence: 99%

Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Thus it seems probable that habituation results from either an increased synthesis or a decreased breakdown of endogenous auxin (Butcher, 1973). A possible reduction in auxin degradation may be associated with a deficiency in auxin degrading peroxidases (Le Dily et al, 1993). Bouchet et al (1978).…”

Section: Discussionmentioning

confidence: 99%

A comparison of normal callus culture and habituated ESM of silver fir (Abies albamill.) for defense reactions to the fungusPhaeolus schweinitzii

Hřib

Vooková

Fl’ak

1996

Archives Of Phytopathology And Plant Protection

View full text Add to dashboard Cite

Normal callus culture and habituated embryogenic suspensor mass (ESM) of silver fir (Abies alba Mill.) were compared enzymatically using three enzyme systems. Significant reduction of peroxidase activity, more intensive glutamate dehydrogenase activity and higher number of isoenzymes of non-specific esterase were found in habituated ESM as compared with the normal calli. Defense reactions of normal callus culture and habituated ESM were tested by a simple method of dual cultures. Habituated ESM showed strengthened defense reactions against tester Phaeolus schweinitzii. Reasons of this phenomenon are discussed.

show abstract

“…There are indications that the plant cancerous cells (as characterized in Table 1) are under permanent stress (Le Dily et al, 1993a) and that light might further stress these achlorophyllous cells . The accumulation of polyamines in cancerous callus cells as well as in hyperhydric shoots might also be indicative of stresses (Smith, 1985).…”

Section: On the Causes Of The Loss Of Organogenic Totipotency In Cancmentioning

confidence: 98%

Special symposium: In vitro plant recalcitrance loss of plant organogenic totipotency in the course of In vitro neoplastic progression

Gaspar

Kevers

Bisbis

et al. 2000

In Vitro Cell.Dev.Biol.-Plant

Self Cite

View full text Add to dashboard Cite

The aptitude for organogenesis from normal hormone-dependent cultures very commonly decreases as the tissues are serially subcultured. The reasons for the loss of regenerative ability may vary under different circumstances: genetic variation in the cell population, epigenetic changes, disappearance of an organogenesis-promoting substance, etc. The same reasons may be evoked for the progressive and eventually irreversible loss of organogenic totipotency in the course of neoplastic progressions from hormone-independent tumors and hyperhydric teratomas to cancers. As in animal cells, plant cells at the end of a neoplastic progression have probably undergone several independent genetic accidents with cumulative effects. They indeed are characterized by atypical biochemical cycles from which they are apparently unable to escape. The metabolic changes are probably not the primary defects that cause cancer, rather they may allow the cells to survive. How these changes, namely an oxidative stress, affect organogenesis is not known. The literature focuses on somatic mutations and epigenetic changes that cause aberrant regulation of cell cycle genes and their machinery

show abstract

Fully habituated sugarbeet callus: Under permanent stress?

Cited by 9 publications

References 47 publications

Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn

Nuclear genome stability in long-term cultivated callus lines of Fagopyrum tataricum (L.) Gaertn

A comparison of normal callus culture and habituated ESM of silver fir (Abies albamill.) for defense reactions to the fungusPhaeolus schweinitzii

Special symposium: In vitro plant recalcitrance loss of plant organogenic totipotency in the course of In vitro neoplastic progression

Contact Info

Product

Resources

About