Function analysis of a semi-dwarf sdg in rice with near isogenic lines*

Li, Xiaobo; Liang, Guohua; Li, Xiazhen; Li, Hongchang; Zhu, Lihuang

doi:10.1080/10020070412331343981

Cited by 1 publication

(2 citation statements)

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“…There is general agreement that GID1 is the only GA receptor, because GA-dependent α-amylase induction scarcely occurs in gid1 aleuronic cells. Moreover, in our previous experiment, α-amylase activity could not be observed in sdg embryoless half seeds after applying GA 3 (Li et al 2004). Thus, it can be deduced that if the GID1 receptor loses function completely or partially, it will influence α-amylase secretion in the aleurone tissue.…”

Section: Discussionmentioning

confidence: 90%

“…Thus, the sdg protein does not show a complete loss of function. To understand the mechanisms by which exogenous GA 3 affects stem elongation, the epidermal cells in the intercalary meristem zone of the sdg mutant and wild-type treated with exogenous GA 3 were observed in our previous experiment (Li et al 2004). In the wild-type material, the cell number dramatically increased and the average longitudinal length of cells treated with GA 3 was about three times that of untreated cells.…”

Section: Discussionmentioning

confidence: 99%

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A New GA-Insensitive Semidwarf Mutant of Rice (Oryza sativa L.) with a Missense Mutation in the SDG Gene

et al. 2011

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A semidwarf line of Indica rice, Xinguiai, was derived from the progeny of a cross between the double dwarf mutant Xinguiaishuangai and the wild-type variety Nanjing 6. The semidwarf phenotype was controlled by the semidwarf gene, sdg. The second sheath and shoot elongation responses of the dwarf mutant to exogenous gibberellin (GA 3 ) showed that sdg was insensitive to gibberellin (GA), and its endogenous GAs content was higher than that in wild-type cultivars. The SDG gene was cloned by a map-based cloning method and sequencing analysis revealed that the coding region of sdg had a single nucleotide substitution resulting in a single amino acid change from alanine to threonine. A cleaved amplified polymorphic sequence marker was designed according to sequences from mutant and wild-type materials. This sequence marker could be used to distinguish wild types and mutants, and thus, could be used for molecular markerassisted selection. The dwarf phenotype of the sdg mutant was restored to a normal phenotype by introducing the wild-type SDG gene. Rice transformation experiments and GUS staining demonstrated that the SDG gene was predominantly expressed in vegetative organs.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%