Function and Fiber-Type Specific Distribution of Hsp60 and αB-Crystallin in Skeletal Muscles: Role of Physical Exercise

D’Amico, Daniela; Fiore, R.; Caporossi, Daniela; Felice, Valentina Di; Cappello, Francesco; Dimauro, Ivan; Barone, Rosario

doi:10.3390/biology10020077

Cited by 8 publications

(6 citation statements)

References 165 publications

(214 reference statements)

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“…Fiber type specificity for Hsp60 was found with high expression in type IIa and type I fibers. The latter was increased by endurance training, which also increased the amount of Hsp60 protein [ 91 ]. In addition to high HSP60 levels, increased mitochondrial copy number and expression of three PGC1α isoforms were also detected [ 92 ].…”

Section: Exercise Training Modulates Cytokines Improving Cachexia Sym...mentioning

confidence: 99%

Cancer-Related Cachexia: The Vicious Circle between Inflammatory Cytokines, Skeletal Muscle, Lipid Metabolism and the Possible Role of Physical Training

Mangano

Fouani

D’Amico

et al. 2022

IJMS

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Cachexia is a multifactorial and multi-organ syndrome that is a major cause of morbidity and mortality in late-stage chronic diseases. The main clinical features of cancer-related cachexia are chronic inflammation, wasting of skeletal muscle and adipose tissue, insulin resistance, anorexia, and impaired myogenesis. A multimodal treatment has been suggested to approach the multifactorial genesis of cachexia. In this context, physical exercise has been found to have a general effect on maintaining homeostasis in a healthy life, involving multiple organs and their metabolism. The purpose of this review is to present the evidence for the relationship between inflammatory cytokines, skeletal muscle, and fat metabolism and the potential role of exercise training in breaking the vicious circle of this impaired tissue cross-talk. Due to the wide-ranging effects of exercise training, from the body to the behavior and cognition of the individual, it seems to be able to improve the quality of life in this syndrome. Therefore, studying the molecular effects of physical exercise could provide important information about the interactions between organs and the systemic mediators involved in the overall homeostasis of the body.

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Section: Exercise Training Modulates Cytokines Improving Cachexia Sym...mentioning

confidence: 99%

Cancer-Related Cachexia: The Vicious Circle between Inflammatory Cytokines, Skeletal Muscle, Lipid Metabolism and the Possible Role of Physical Training

Mangano

Fouani

D’Amico

et al. 2022

IJMS

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“…Other fibers have slow twitches and shortening velocities, but are fatigue resistant and have a low energy expenditure. Such fibers are used for tasks like keeping body and limb posture [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Comparing the epigenetic landscape in myonuclei purified with a PCM1 antibody from a fast/glycolytic and a slow/oxidative muscle

et al. 2021

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Muscle cells have different phenotypes adapted to different usage, and can be grossly divided into fast/glycolytic and slow/oxidative types. While most muscles contain a mixture of such fiber types, we aimed at providing a genome-wide analysis of the epigenetic landscape by ChIP-Seq in two muscle extremes, the fast/glycolytic extensor digitorum longus (EDL) and slow/oxidative soleus muscles. Muscle is a heterogeneous tissue where up to 60% of the nuclei can be of a different origin. Since cellular homogeneity is critical in epigenome-wide association studies we developed a new method for purifying skeletal muscle nuclei from whole tissue, based on the nuclear envelope protein Pericentriolar material 1 (PCM1) being a specific marker for myonuclei. Using antibody labelling and a magnetic-assisted sorting approach, we were able to sort out myonuclei with 95% purity in muscles from mice, rats and humans. The sorting eliminated influence from the other cell types in the tissue and improved the myo-specific signal. A genome-wide comparison of the epigenetic landscape in EDL and soleus reflected the differences in the functional properties of the two muscles, and revealed distinct regulatory programs involving distal enhancers, including a glycolytic super-enhancer in the EDL. The two muscles were also regulated by different sets of transcription factors; e.g. in soleus, binding sites for MEF2C, NFATC2 and PPARA were enriched, while in EDL MYOD1 and SIX1 binding sites were found to be overrepresented. In addition, more novel transcription factors for muscle regulation such as members of the MAF family, ZFX and ZBTB14 were identified.

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“…In adult skeletal muscle, HSPB5 is associated with the cytoskeletal structures at the level of Z-bands (e.g., actin and titin), and it is highly expressed in slow and fast fibers [ 23 , 62 , 89 ]. Many different lines of evidence suggest a protective role of this sHSP in mammalian skeletal muscle from heat, oxidative, and mechanical stresses produced during middle age and senescence or by physical activity [ 58 , 62 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 ].…”

Section: Physiological Roles Of Hspb5 In Skeletal and Cardiac Musclementioning

confidence: 99%

Alpha B-Crystallin in Muscle Disease Prevention: The Role of Physical Activity

Dimauro

Caporossi

2022

Molecules

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HSPB5 or alpha B-crystallin (CRYAB), originally identified as lens protein, is one of the most widespread and represented of the human small heat shock proteins (sHSPs). It is greatly expressed in tissue with high rates of oxidative metabolism, such as skeletal and cardiac muscles, where HSPB5 dysfunction is associated with a plethora of human diseases. Since HSPB5 has a major role in protecting muscle tissues from the alterations of protein stability (i.e., microfilaments, microtubules, and intermediate filament components), it is not surprising that this sHSP is specifically modulated by exercise. Considering the robust content and the protective function of HSPB5 in striated muscle tissues, as well as its specific response to muscle contraction, it is then realistic to predict a specific role for exercise-induced modulation of HSPB5 in the prevention of muscle diseases caused by protein misfolding. After offering an overview of the current knowledge on HSPB5 structure and function in muscle, this review aims to introduce the reader to the capacity that different exercise modalities have to induce and/or activate HSPB5 to levels sufficient to confer protection, with the potential to prevent or delay skeletal and cardiac muscle disorders.

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Function and Fiber-Type Specific Distribution of Hsp60 and αB-Crystallin in Skeletal Muscles: Role of Physical Exercise

Cited by 8 publications

References 165 publications

Cancer-Related Cachexia: The Vicious Circle between Inflammatory Cytokines, Skeletal Muscle, Lipid Metabolism and the Possible Role of Physical Training

Cancer-Related Cachexia: The Vicious Circle between Inflammatory Cytokines, Skeletal Muscle, Lipid Metabolism and the Possible Role of Physical Training

Comparing the epigenetic landscape in myonuclei purified with a PCM1 antibody from a fast/glycolytic and a slow/oxidative muscle

Alpha B-Crystallin in Muscle Disease Prevention: The Role of Physical Activity

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