Function and regulation in MAPK signaling pathways: Lessons learned from the yeast Saccharomyces cerevisiae

Chen, Raymond E.; Thorner, Jeremy

doi:10.1016/j.bbamcr.2007.05.003

Cited by 570 publications

(620 citation statements)

References 391 publications

(535 reference statements)

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“…In S. cerevisiae, the signals and receptors triggering the mating response of the alternative mating types differ, as also occurs in other yeast (Leberer et al, 1997;Elion, 2000;Davidson et al, 2003;Li et al, 2005). In S. cerevisiae, the signals, receptors, and the majority of components of the signal transduction pathways differ among several environmental responses, including the mating response to pheromones, filamentation, ascospore formation, and osmoregulation (Gustin et al, 1998;Chen and Thorner, 2007). However, we found no definitive example in other fungi of the scenario we have described for white and opaque cells of C. albicans, specifically, that the same signal interacts with the same receptor, activating the same upstream signal transduction pathway, but different downstream regulators in two different cell types, resulting in completely different responses.…”

Section: Discussionmentioning

confidence: 99%

“…The mitogen-activated protein (MAP) kinase pathways have evolved as highly efficient, multipurpose signal transduction systems. S. cerevisiae uses multiple MAP kinase pathways, each one for a distinct signaling system, including the mating process, the filamentation process, cell wall integrity, ascospore formation, and osmoregulation (Levin and Errede, 1995;Gustin et al, 1998;Saito and Tatebayashi, 2004;Chen and Thorner, 2007). Several of these pathways share a limited number of components, but all are presumed to use different receptors to elicit very different responses.…”

Section: Introductionmentioning

confidence: 99%

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The Same Receptor, G Protein, and Mitogen-activated Protein Kinase Pathway Activate Different Downstream Regulators in the Alternative White and Opaque Pheromone Responses ofCandida albicans

Song

Sahni

Daniels

et al. 2008

MBoC

251

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Candida albicans must undergo a switch from white to opaque to mate. Opaque cells then release mating type-specific pheromones that induce mating responses in opaque cells. Uniquely in C. albicans, the same pheromones induce mating-incompetent white cells to become cohesive, form an adhesive basal layer of cells on a surface, and then generate a thicker biofilm that, in vitro, facilitates mating between minority opaque cells. Through mutant analysis, it is demonstrated that the pathways regulating the white and opaque cell responses to the same pheromone share the same upstream components, including receptors, heterotrimeric G protein, and mitogen-activated protein kinase cascade, but they use different downstream transcription factors that regulate the expression of genes specific to the alternative responses. This configuration, although common in higher, multicellular systems, is not common in fungi, and it has not been reported in Saccharomyces cerevisiae. The implications in the evolution of multicellularity in higher eukaryotes are discussed. INTRODUCTIONBoth single cell organisms and the individual cells of multicellular organisms must respond to a variety of environmental signals (Dorsky et al., 2000;Balázsi and Oltvai, 2005). In addition, in higher eukaryotes different cell types frequently respond to the same signal in unique ways (Rincón and Pedraza-Alva, 2003;Bacci et al., 2005;Dailey et al., 2005). In most cases, different signals interact with unique surface receptors that activate different signal transduction pathways, as has been demonstrated in Saccharomyces cerevisiae (Leberer et al., 1997;Gustin et al., 1998;Madhani and Fink, 1998;Elion, 2000). The mitogen-activated protein (MAP) kinase pathways have evolved as highly efficient, multipurpose signal transduction systems. S. cerevisiae uses multiple MAP kinase pathways, each one for a distinct signaling system, including the mating process, the filamentation process, cell wall integrity, ascospore formation, and osmoregulation (Levin and Errede, 1995;Gustin et al., 1998;Saito and Tatebayashi, 2004;Chen and Thorner, 2007). Several of these pathways share a limited number of components, but all are presumed to use different receptors to elicit very different responses. In the mating process of S. cerevisiae, a cells release a-factor that interacts with the a-receptor on ␣ cells, and ␣ cells release ␣-factor that interacts with the ␣-receptor on a cells. These alternative signals are then transduced through the same heterotrimeric G protein to activate the same MAP kinase pathway, which in turn activates the same downstream regulators that elicit similar mating responses, including G1 arrest, polarization, and shmooing (Sprague et al., 1983;Bender and Sprague, 1986;Leberer et al., 1997). Other fungi, including Magnaporthe grisea (Dixon et al., 1999;Zhao et al., 2005b) Neurospora crassa (Li et al., 2005), and Cryptococcus neoformans (Davidson et al., 2003;Kraus et al., 2003;Bahn et al., 2005) also use MAP kinase pathways for a variety of responses...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Same Receptor, G Protein, and Mitogen-activated Protein Kinase Pathway Activate Different Downstream Regulators in the Alternative White and Opaque Pheromone Responses ofCandida albicans

Song

Sahni

Daniels

et al. 2008

MBoC

251

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“…Mechanisms used in yeast to transduce information from the exterior to the interior of the cells are also present in higher eukaryotes. As an example, the mitogen activated protein kinase (MAPK) pathway, which consists of a module of three kinases that activate each other is conserved from yeasts to mammals 24 . Misregulation of MAPK signal transduction in mammalians cells has been implicated in multiple diseases such as cancer and inflammation.…”

Section: Budding Yeast As a Model Organismmentioning

confidence: 99%

Fluorescent-Based Quantitative Measurements of Signal Transduction in Single Cells

Pelet¹,

Peter²

2011

Design and Analysis of Biomolecular Circuits

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Budding yeast (Saccharomyces cerevisiae) has been widely used as a model system to study fundamental biological processes. Genetic and biochemical approaches have allowed in the last decades to uncover the key components involved in many signaling pathways. Generally, most techniques measure the average behavior of a population of cells, and thus missed important cell-to-cell variations. With the recent progress with fluorescent proteins, new avenues have been opened to quantitatively study the dynamics of signaling in single living cells. In this chapter, we describe several techniques based on fluorescence measurements to quantify the activation of biological pathways. Flow cytometry allows for rapid quantification of the total fluorescence of a large number of single cells. In contrast, microscopy offers a lower throughput but allows to follow with a high temporal resolution the localization of proteins at sub-cellular resolution. Finally, advanced functional imaging techniques such as FRET and FCS offer the possibility to directly visualize the formation of protein complexes or to quantify the activity of proteins in vivo. Together these techniques present powerful new approaches to study cellular signaling and will greatly increase our understanding of the regulation of signaling networks in budding yeast and beyond. FLUORESCENT-BASED QUANTITATIVE MEASUREMENTS OF SIGNAL TRANSDUCTION IN SINGLE CELLS Serge Pelet and Matthias Peter Institute of Biochemistry, Department of Biology, ETH Zürich Abstract:Budding yeast (Saccharomyces cerevisiae) has been widely used as a model system to study fundamental biological processes. Genetic and biochemical approaches have allowed in the last decades to uncover the key components involved in many signaling pathways. Generally, most techniques measure the average behavior of a population of cells, and thus missed important cell-tocell variations. With the recent progress with fluorescent proteins, new avenues have been opened to quantitatively study the dynamics of signaling in single living cells. In this chapter, we describe several techniques based on fluorescence measurements to quantify the activation of biological pathways. Flow cytometry allows for rapid quantification of the total fluorescence of a large number of single cells. In contrast, microscopy offers a lower throughput but allows to follow with a high temporal resolution the localization of proteins at sub-cellular resolution. Finally, advanced functional imaging techniques such as FRET and FCS offer the possibility to directly visualize the formation of protein complexes or to quantify the activity of proteins in vivo.Together these techniques present powerful new approaches to study cellular signaling and will greatly increase our understanding of the regulation of signaling networks in budding yeast and beyond.

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“…MAPK pathways, which are remarkably well conserved through evolution, regulate key cellular processes, such as cellular proliferation, differentiation and development, apoptosis, motility or response to various stresses [50]. As shown in Figure 1, the yeast Saccharomyces cerevisiae has five MAPKs, Fus3, Kss1, Hog1, Slt2/Mpk1 and Smk1, involved in the pheromone, filamentous and invasive growth, high osmolarity (HOG), cell wall integrity (CWI) and spore wall assembly pathways, respectively [8].…”

Section: Introductionmentioning

confidence: 99%

Fine regulation of Saccharomyces cerevisiae MAPK pathways by post‐translational modifications

Molina

Cid

Martı́n

2010

Yeast

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Saccharomyces cerevisiae has been widely used as a model eukaryotic organism to elucidate the molecular mechanisms that operate upon activation of signalling pathways. For over two decades, many clues to the regulation of mitogen-activated protein kinase (MAPK) pathways have derived from basic research in yeast. Here we review aspects of MAPK pathway fine-tuning, such as the functional implication of feedback loops or regulatory inputs from other pathways, mediated by post-transcriptional modifications on their components. The impact of recent phosphoproteomic approaches in this particular field is also discussed.

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Function and regulation in MAPK signaling pathways: Lessons learned from the yeast Saccharomyces cerevisiae

Cited by 570 publications

References 391 publications

The Same Receptor, G Protein, and Mitogen-activated Protein Kinase Pathway Activate Different Downstream Regulators in the Alternative White and Opaque Pheromone Responses ofCandida albicans

The Same Receptor, G Protein, and Mitogen-activated Protein Kinase Pathway Activate Different Downstream Regulators in the Alternative White and Opaque Pheromone Responses ofCandida albicans

Fluorescent-Based Quantitative Measurements of Signal Transduction in Single Cells

Fine regulation of Saccharomyces cerevisiae MAPK pathways by post‐translational modifications

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