Function of adult pig hearts after 2 and 12 hours of cold cardioplegic preservation

Budrikis, Algimantas; Bolys, Ramūnas; Liao, Quiming; Ingemansson, Richard; Sjöberg, Trygve; Steen, Stig

doi:10.1016/s0003-4975(98)00316-6

Cited by 14 publications

(17 citation statements)

References 14 publications

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“…Disturbance of the functional integrity of the vascular endothelium, known as "endothelium dysfunction", plays a significant role in myocardial ischemia-reperfusion injury (3)(4)(5)(6)(10)(11)(12). The vascular endothelium is a single layer of cells that lines the entire circulatory system.…”

Section: Discussionmentioning

confidence: 99%

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Intact endothelial and contractile function of coronary artery after 8 hours of heart preservation

Qin

Sjöberg

Liao

et al. 2016

Scandinavian Cardiovascular Journal

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Objectives. The aim of the study was to investigate if adequate preservation of coronary artery endothelium-dependent relaxation and contractility may be obtained after 8 hours of non-ischemic heart preservation.Design. Porcine hearts were perfused for 8 hours at 8°C, either in cycles of 15 minutes perfusion and 60 minutes non-perfusion, or by continuous perfusion. The perfusate consisted of a cardioplegic, hyperoncotic nutrition solution with oxygenated red cells, and the perfusion pressure was 20 mmHg. In organ baths, coronary artery segments from the preserved hearts were studied and compared to fresh controls.Results. Endothelium-dependent relaxation and contractility were fully preserved after both intermittent and continuous perfusion, as compared to fresh controls. No myocardial edema was seen; water content of the myocardium was 79.5±0.2%, 79.0±0.4% and 79.0±0.3% (ns) for fresh controls, intermittently perfused, and continuously perfused hearts, respectively.

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Section: Discussionmentioning

confidence: 99%

“…A correlation was found (p<0.001) between high coronary vascular resistance and low EDR (3) and the coronary flow and cardiac output were significantly reduced in hearts with low EDR (4). The endothelium thus plays a critical role in the maintenance of normal vessel function.…”

Section: Introductionmentioning

confidence: 95%

Intact endothelial and contractile function of coronary artery after 8 hours of heart preservation

Qin

Sjöberg

Liao

et al. 2016

Scandinavian Cardiovascular Journal

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“…Budrikis et al [10], in studies with cold crystalloid solution with isolated working pig heart model, report the importance of experimental observation over long periods such as 12 hours, in order to verify differences in hemodynamic parameters of ischemia and reperfusion in animal models. Although no tendency has been observed in this study that may assume any change in the obtained results, it is clear that longer periods of observation may always show not easily suspectable results.…”

Section: Discussionmentioning

confidence: 99%

“…The oxygen consumption was obtained by means of the difference between the arterial blood sample from the animal support and the blood collected from the coronary sinus in the right ventricle cannula, according to the formula: MVO2 = 0.1 X Coronary flow / Final Weight of the left ventricle X [(SO2a -SO2v) X 1.34 X Hb / 100 + 0.03 X 7,5 X (pO2a -pO2v)], where MVO2 and consumption of oxygen (expressed by 100g of myocardium, SO2a and SO2v) are the oxygen saturation (in %) from the animal support and the coronary sinus of the isolated working heart respectively; Hb and the hemoglobin volume (in g/L) and pO2a and pO2v are the partial pressures of oxygen from the animal support and the coronary sinus of the isolated working heart, respectively [10].…”

Section: Measurements Obtained With Isolated Working Heart In Workingmentioning

confidence: 99%

Trimetazidina como aditivo em solução cardioplégica sem pré-tratamento não traz proteção adicional ao miocárdio isquêmico: estudo em modelo suíno de coração isolado

Filho¹,

Petrucci²,

Carmo³

et al. 2008

Rev Bras Cir Cardiovasc

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“…The oxygen consumption was calculated as the difference between the arterial blood sample from the support animal and the blood collected from the coronary sinus in the right ventricle cannula according to the following formula: MVO2 = 0.1 X coronary flow / Final weight of the left ventricle X ((SO 2a -SO 2v ) x 1,34 x Hb/100 + 0.03 x 7,5 x (pO 2a -pO 2v )), where MVO 2 and oxygen consumption of oxygen express per 100g of myocardium, SO 2a and SO 2v are the oxygen saturation (in %) of the support animal and the coronary sinus of the isolated heart, respectively; Hb and hemoglobin volume (in g/L) and pO 2a and pO 2v are the partial oxygen pressure from the support animal and the coronary sinus from the isolated heart, respectively [9].…”

Section: Isolated Heart Reperfusionmentioning

confidence: 99%

Padronização de modelo de coração isolado "working heart" com circulação parabiótica

Filho¹,

Petrucci²,

Carmo³

et al. 2008

Rev Bras Cir Cardiovasc

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Objective: To develop an isolated working heart model with parabiotic circulation in swine and to verify its stability and possibility for allowing effective measurements of hemodynamic and metabolic data.Methods: This model was developed during an association study of cardiolegia agents. Eighteen experiments were performed, each with a support animal and a donor animal. Donor animal heart was perfused as isolated working heart with parabiotic circulation from the support animal. The isolated heart underwent regional ischemia by interventricular artery clamping, followed by global ischemia. During reperfusion in a isolated heart in working state at 30, 60, and 90 minutes, contractility indices such as elastance, preload recruitable stroke work index, and metabolic data were acquired.Results: Support animals were kept stable throughout the procedures without use of blood transfusions or vasoactive drugs. Variables such as pH, oxygen partial pressure and hematocrit were kept stable and within physiologic ranges. The isolated heart was perfused adequately throughout the experiment. All hemodynamic and metabolic data proposed were adequately measured in the isolated heart in working state.Conclusion: This isolated swine "working heart" model was kept stable throughout the experiments with no administration of vasoactive drugs, and it allowed adequate measurements of metabolic and hemodynamic data.Descriptors: Heart/surgery. Models, animal. Swine. Cardioplegic solutions. Cardiac surgical procedures. Myocardial reperfusion/methods. 15SILVEIRA FILHO, LM ET AL -Development of isolated swine "working heart model" with parabiotic circulation Bras Cir Cardiovasc 2008; 23(1): 14-22 Rev

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Function of adult pig hearts after 2 and 12 hours of cold cardioplegic preservation

Cited by 14 publications

References 14 publications

Intact endothelial and contractile function of coronary artery after 8 hours of heart preservation

Intact endothelial and contractile function of coronary artery after 8 hours of heart preservation

Trimetazidina como aditivo em solução cardioplégica sem pré-tratamento não traz proteção adicional ao miocárdio isquêmico: estudo em modelo suíno de coração isolado

Padronização de modelo de coração isolado "working heart" com circulação parabiótica

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