Function of Laminins and Laminin‐Binding Integrins In Gingival Epithelial Cell Adhesion

Abstract

Our results provide new information on gingival epithelial cell adhesion and extracellular matrix production and may thus aid in the understanding of periodontal physiology.

Expression and function of laminin and integrins on adhesion/migration of primary culture cells derived from rat oral epithelium

“…This result is consistent with the results of the study by Pakkala et al. (25) that reported intense expression of laminin γ 2 as a belt‐and‐bow‐like immunofluorescence at the peripheral cytoplasm. Likewise, the x–z axis images revealed intense immunoreactivity for laminin γ 2 in the basal region of cells adjacent to the wound area.…”

Human laminin‐332 degradation by Candida proteinases

“…Specific proteolytic processing of Lm‐332 α3 chains causes polymerization and hemidesmosome formation. We propose that further processing of the γ2 chain to 105 kDa (11) can cause a migratory effect on keratinocytes.…”

“…The human spontaneous immortalized mucosal keratinocyte cell line (22) was used. This cell line has been shown to secrete Lm‐332 into the culture medium (11). This cell line has been successfully used in numerous studies and it appears to retain its properties.…”

“…Immunoprecipitation of Lm‐332 was performed according to Pakkala et al. (11) with slight modifications. Briefly, the cells were labelled overnight with 35 S‐methionine (25 μCi / ml; Amersham Biosciences, Uppsala, Sweden).…”

Biological activation of zirconia surfaces by chemical modification with IGF‐1

“…1 Laminin-5 plays an important role in the migration, adhesion, proliferation, and differentiation of epithelial cells, and is also a component of the filaments that anchor hemidesmosomes. 2,3 Human gingival epithelia are known to express several laminin-binding integrin receptors, such as hemidesmosomal integrin complex a6b4, 4 which is also a receptor of laminin-5 and has been reported to play an important role in cell adhesion. 5 It is generally accepted that the installation of osseointegrated dental implants in partially edentulous patients is an effective therapy for supplementing natural teeth with implant-supported structures.…”