Function of Sox2 in ependymal cells of lesioned spinal cords in adult zebrafish

Ogai, Kazuhiro; Nakatani, Kumi; Hisano, Suguru; Sugitani, Kayo; Koriyama, Yoshiki; Kato, Satoru

doi:10.1016/j.neures.2014.07.010

Cited by 35 publications

(31 citation statements)

References 19 publications

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“…Sox2 plays an important role in various biological processes of vertebrates. In addition to regulating the properties of stem cells, Sox2 is also involved in neuronal formation, differentiation, proliferation, and development (Zappone et al, 2000;Ogai et al, 2014). However, little is known about the role of Sox2 in motor neuron development.…”

Section: Discussionmentioning

confidence: 99%

The Requirement of Sox2 for the Spinal Cord Motor Neuron Development of Zebrafish

Gong

Huang

et al. 2020

Front. Mol. Neurosci.

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Sex-determining region Y box 2 (Sox2), expressed in neural tissues, plays an important role as a transcription factor not only in the pluripotency and proliferation of neuronal cells but also in the opposite function of cell differentiation. Nevertheless, how Sox2 is linked to motor neuron development remains unknown. Here, we showed that Sox2 was localized in the motor neurons of spinal cord by in situ hybridization and cell separation, which acted as a positive regulator of motor neuron development. The deficiency of Sox2 in zebrafish larvae resulted in abnormal PMN development, including truncated but excessively branched CaP axons, loss of MiP, and increase of undifferentiated neuron cells. Importantly, transcriptome analysis showed that Sox2-depleted embryos caused many neurogenesis, axonogenesis, axon guidance, and differentiation-related gene expression changes, which further support the vital function of Sox2 in motor neuron development. Taken together, these data indicate that Sox2 plays a crucial role in the motor neuron development by regulating neuron differentiation and morphology of neuron axons.

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Section: Discussionmentioning

confidence: 99%

The Requirement of Sox2 for the Spinal Cord Motor Neuron Development of Zebrafish

Gong

Huang

et al. 2020

Front. Mol. Neurosci.

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“…sox2 is also important for spinal cord regeneration in larval Xenopus (Gaete et al, 2012) and lesion-induced ERG proliferation in the adult lesioned zebrafish spinal cord (Ogai et al, 2014).…”

Section: Types and Extent Of Neuronal Regeneration In The Cns Of Anammentioning

confidence: 99%

Neuronal Regeneration from Ependymo-Radial Glial Cells: Cook, Little Pot, Cook!

Becker

2015

Developmental Cell

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Adult fish and salamanders regenerate specific neurons as well as entire CNS areas after injury. Recent studies shed light on how these anamniotes activate progenitor cells, generate the required cell types, and functionally integrate these into a complex environment. Some developmental signals and mechanisms are recapitulated during neuronal regeneration, whereas others are unique to the regeneration process. The use of genetic techniques, such as cell ablation and lineage-tracing, in combination with cell-type-specific expression profiling reveal factors that initiate, fine-tune, and terminate the regenerative response in anamniotes, with a view to translating findings to non-regenerating species.

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“…A standard MO (5′-CCT CTT ACC TCA GTT ACA ATT TAT A-32) was used as a control [42]. All MOs were purchased from Gene Tools.…”

Section: Methodsmentioning

confidence: 99%

Loss ofZNF32augments the regeneration of nervous lateral line system through negative regulation ofSOX2transcription

Wei

Yao

et al. 2016

Oncotarget

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Human zinc finger protein 32 (ZNF32) is a Cys2-His2 zinc-finger transcription factor that plays an important role in cell fate, yet much of its function remains unknown. Here, we reveal that the zebrafish ZNF32 homologue zfZNF32 is expressed in the nervous system, particularly in the lateral line system. ZfZNF32 knock-out zebrafish (zfZNF−/−) were generated using the CRISPR-associated protein 9 system. We found that the regenerative capacity of the lateral line system was increased in zfZNF−/− upon hair cell damage compared with the wild type. Moreover, SOX2 was essential for the zfZNF32-dependent modulation of lateral line system regeneration. Mechanistic studies showed that ZNF32 suppressed SOX2 transcription by directly binding to a consensus sequence (5′-gcattt-32) in the SOX2 promoter. In addition, ZNF32 localizes to the nucleus, and we have identified that amino acids 1-169 (Aa 1-169) and each of three independent nuclear localization signals (NLSs) in ZNF32 are indispensable for ZNF32 nuclear trafficking. Mutating the NLSs disrupted the inhibitory effect of ZNF32 in SOX2 expression, highlighting the critical role of the NLSs in ZNF32 function. Our findings reveal a pivotal role for ZNF32 function in SOX2 expression and regeneration regulation.

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Function of Sox2 in ependymal cells of lesioned spinal cords in adult zebrafish

Cited by 35 publications

References 19 publications

The Requirement of Sox2 for the Spinal Cord Motor Neuron Development of Zebrafish

The Requirement of Sox2 for the Spinal Cord Motor Neuron Development of Zebrafish

Neuronal Regeneration from Ependymo-Radial Glial Cells: Cook, Little Pot, Cook!

Loss ofZNF32augments the regeneration of nervous lateral line system through negative regulation ofSOX2transcription

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