Functional analysis and screening small molecules to RpfF protein in Xanthomonas oryzae involved in rice bacterial blight disease

Srilatha, M.; Patyal, Naina; Saddala, Madhu Sudhana

doi:10.1016/s2095-3119(19)62813-3

Cited by 12 publications

(7 citation statements)

References 39 publications

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“…In 2020, Srilatha et al predicted the 3D structure of the RpfF protein using homology modeling and docked rifampicin analogues within the active site of RpfF by virtual screening in order to identify potent inhibitors [ 62 ]. Some of the screened compounds from the ZINC database had higher energies and lower toxicity than the reference compound rifampicin.…”

Section: Dsf Biosynthasementioning

confidence: 99%

“… Kanugala et al [ 61 ] Chumacin-2 Rifampicin analogues Virtual screening form ZIN Database Docked into the active site of the RpfF protein. Srilatha et al [ 62 ] Coumarin derivative Synthesis based on derivation of natural product Disturbed biofilm formation; suppressed bacterial virulence factors and production of DSF; reduced expression of rpfF gene. Feng et al [ 64 ] …”

Section: Dsf Biosynthasementioning

confidence: 99%

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Research on Diffusible Signal Factor-Mediated Quorum Sensing in Xanthomonas: A Mini-Review

et al. 2023

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Xanthomonas spp. are important plant pathogens that seriously endanger crop yields and food security. RpfF is a key enzyme that is involved in the synthesis of diffusible signal factor (DSF) signals and predominates in the signaling pathway regulating quorum sensing (QS) in Xanthomonas. Currently, novel RpfF enzyme-based quorum sensing agents have been proposed as a promising strategy for the development of new pesticides. However, few reports are available that comprehensively summarize the progress in this field. Therefore, we provide a comprehensive review of the recent advances in DSF-mediated QS and recently reported inhibitors that are proposed as bactericide candidates to target the RpfF enzyme and control plant bacterial diseases.

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Section: Dsf Biosynthasementioning

confidence: 99%

Section: Dsf Biosynthasementioning

confidence: 99%

Research on Diffusible Signal Factor-Mediated Quorum Sensing in Xanthomonas: A Mini-Review

et al. 2023

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“…• Activation of RpfC by diffusible signal factor (DSF) signals including DSF, BDSF, CDSF and IDSF [70] • Virulence for Xanthomonas oryzae [71] and Xanthomonas campestris pv. campestris [72].…”

Section: Rpfc-rpfgmentioning

confidence: 99%

Evolution of two-component quorum sensing systems

Giannakara

Koumandou

2022

Access Microbiology

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Quorum sensing (QS) is a cell-to-cell communication system that enables bacteria to coordinate their gene expression depending on their population density, via the detection of small molecules called autoinducers. In this way bacteria can act collectively to initiate processes like bioluminescence, virulence and biofilm formation. Autoinducers are detected by receptors, some of which are part of two-component signal transduction systems (TCS), which comprise of a (usually membrane-bound) sensor histidine kinase (HK) and a cognate response regulator (RR). Different QS systems are used by different bacterial taxa, and their relative evolutionary relationships have not been extensively studied. To address this, we used the Kyoto Encyclopedia of Genes and Genomes (KEGG) database to identify all the QS HKs and RRs that are part of TCSs and examined their conservation across microbial taxa. We compared the combinations of the highly conserved domains in the different families of receptors and response regulators using the Simple Modular Architecture Research Tool (SMART) and KEGG databases, and we also carried out phylogenetic analyses for each family, and all families together. The distribution of the different QS systems across taxa, indicates flexibility in HK–RR pairing and highlights the need for further study of the most abundant systems. For both the QS receptors and the response regulators, our results indicate close evolutionary relationships between certain families, highlighting a common evolutionary history which can inform future applications, such as the design of novel inhibitors for pathogenic QS systems.

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“…In 2020, Srilatha et al used in silico docking analysis to screen for compounds that interact with RpfF and exhibit less toxicity than reference compound DSFs. To our knowledge, this is the only report identifying an RpfF-targeting small molecule . Although the design of RpfF-targeting small molecules is challenging, studies of various QS inhibitors have provided new insights that could facilitate the discovery of potentially useful agents.…”

Section: Introductionmentioning

confidence: 99%

“…To our knowledge, this is the only report identifying an RpfF-targeting small molecule. 18 Although the design of RpfF-targeting small molecules is challenging, studies of various QS inhibitors have provided new insights that could facilitate the discovery of potentially useful agents. For instance, many azole compounds exhibit excellent antibacterial and potentially strong anti-QS activity.…”

Section: Introductionmentioning

confidence: 99%

Novel Benzothiazole Derivatives as Potential Anti-Quorum Sensing Agents for Managing Plant Bacterial Diseases: Synthesis, Antibacterial Activity Assessment, and SAR Study

Chu

Yang

Long

et al. 2023

J. Agric. Food Chem.

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As quorum sensing (QS) regulates bacterial pathogenicity, antiquorum sensing agents have powerful application potential for controlling bacterial infections and overcoming pesticide/drug resistance. Identifying anti-QS agents thus represents a promising approach in agrochemical development. In this study, the anti-QS potency of 53 newly prepared benzothiazole derivatives containing an isopropanolamine moiety was analyzed, and structure−activity relationships were examined. Compound D 3 exhibited the strongest antibacterial activity, with an in vitro EC 50 of 1.54 μg mL −1 against Xanthomonas oryzae pv oryzae (Xoo). Compound D 3 suppressed QS-regulated virulence factors (e.g., biofilm, extracellular polysaccharides, extracellular enzymes, and flagella) to inhibit bacterial infection. In vivo anti-Xoo assays indicated good control efficiency (curative activity, 47.8%; protective activity, 48.7%) at 200 μg mL −1 . Greater control efficiency was achieved with addition of 0.1% organic silicone or orange peel essential oil. The remarkable anti-QS potency of these benzothiazole derivatives could facilitate further novel bactericidal compound development.

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Functional analysis and screening small molecules to RpfF protein in Xanthomonas oryzae involved in rice bacterial blight disease

Cited by 12 publications

References 39 publications

Research on Diffusible Signal Factor-Mediated Quorum Sensing in Xanthomonas: A Mini-Review

Research on Diffusible Signal Factor-Mediated Quorum Sensing in Xanthomonas: A Mini-Review

Evolution of two-component quorum sensing systems

Novel Benzothiazole Derivatives as Potential Anti-Quorum Sensing Agents for Managing Plant Bacterial Diseases: Synthesis, Antibacterial Activity Assessment, and SAR Study

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