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Background: The rising burden of cancer worldwide calls for an alternative treatment solution. Herbal medicine provides a very feasible alternative to western medicine against cancer.The present study assessed the Objectives: in vitro Tabernaemontana divaricata . antioxidative potential and cytotoxicity of leaves protein fraction Materials and Methods: The antioxidant activity of protein fraction of was performed by several antioxidative assays T. divaricata including 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay, ABTS, superoxide radical (SO) scavenging assay and Hydrogen peroxide (H O ) with standard protocol, followed by Cytotoxicity test with tryphan 2 2 blue assay.From the results, has been found to have the significant antioxidant activity in a dose-Results:T.divaricata dependent manner and IC value was 59.1μg/ml for DPPH, 48 µg/ml for ABTS, 60 µg/ml for SO and 38.5 µg/ml for 50 H O . Further, the cytotoxicity analysis was determined against Dalton Lymphoma Ascites (DLA) cell line and the IC 2 2 50 value was found to be 62μg/ml for protein fraction of Hence, the current study attests that T.divaricata.T. Conclusion:divaricata is a fine source of natural antioxidants with anticancer agents and can be used in pharmaceutical preparations for the treatment of diseases induced by oxidative stress.

Objective: Tabernaemontana divaricata (Td) is commonly used plants for the treatment of inflammatory, anticancer, and diabetics. The main objective of this study is to assess the antitumor and antioxidant activity of Td in mice model. Methods:Antitumor activity of TdPf extract (52 µg/ml) is evaluated against Dalton's lymphoma ascites (DLA) tumor mice. After 24 hrs of tumor inoculation, the extract is administered daily for 15 and 60 days. After administration of the lost dose followed by 18 hrs fasting, mice are sacrificed for observation of antitumor activity. Antitumor activity is assessed by monitoring the liver marker enzyme, lipid peroxidation effect on antioxidant enzyme levels, and histopathological evidence. Results:The results showed that the protein extract of Td animals restored the antioxidant enzymes when compared to the mice of the DLA control group. Conclusion:The findings indicate that the extract of plant protein has antitumor activity by preventing the lipid peroxidation and thereby promoting the antioxidant systems in DLA induced mice. And hence, it is evident that these extract could be a natural anticancer agent for the human health.