Functional analysis of a cold-responsive rice WRKY gene, OsWRKY71

Kim, Chi-Yeol; Vo, Kieu Thi Xuan; Nguyen, Cong Danh; Jeong, Dong‐Hoon; Lee, Sang‐Kyu; Kumar, Manu; Kim, Sung‐Ryong; Park, Suhyun; Kim, Ju-Kon; Jeon, Jong‐Seong

doi:10.1007/s11816-015-0383-2

Cited by 102 publications

(86 citation statements)

References 74 publications

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“…The WRKY transcription factors (TFs) play pivotal roles in plant biotic and abiotic stress responses (Rushton et al ., ; Chen et al ., ; Phukan et al ., ), including plant defense (Eulgem & Somssich, ; Pandey & Somssich, ), cold stress (Wang et al ., ; Kim et al ., ), salt stress (Jiang & Deyholos, ; Niu et al ., ), drought stress (Wu et al ., ; Ren et al ., ; Tripathi et al ., ) and wounding stress (Hara et al ., ; Skibbe et al ., ). Among various stress responses, the roles of WRKY TFs in wounding stress response are largely unknown.…”

Section: Discussionmentioning

confidence: 99%

MdWRKY40 promotes wounding‐induced anthocyanin biosynthesis in association with MdMYB1 and undergoes MdBT2‐mediated degradation

et al. 2019

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Summary Wounding stress leads to anthocyanin accumulation. However, the underlying molecular mechanism remains elusive. In this study, MdWRKY40 was found to promote wounding‐induced anthocyanin biosynthesis in association with MdMYB1 and undergo MdBT2‐mediated degradation in apple. We found that MdMYB1, a positive regulator of anthocyanin biosynthesis, was essential for the wounding‐induced anthocyanin biosynthesis in apple. MdWRKY40 was identified as an MdMYB1‐interacting protein, and enhanced the binding of MdMYB1 to its target genes in response to wounding. We found that MdBT2 interacted physically with MdWRKY40 and was involved in its degradation through the 26S proteasome pathway. Our results demonstrate that MdWRKY40 is a key modulator in the wounding‐induced anthocyanin biosynthesis, which provides new insights into the regulation of wounding‐induced anthocyanin biosynthesis at both the transcriptional and post‐translational levels in apple.

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Section: Discussionmentioning

confidence: 99%

MdWRKY40 promotes wounding‐induced anthocyanin biosynthesis in association with MdMYB1 and undergoes MdBT2‐mediated degradation

et al. 2019

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“…We searched the promoter sequences upstream of the 5′‐end of full‐length OsNAC2 cDNA against the PLACE database to identify putative cis ‐acting regulatory DNA elements. Several cis ‐elements were found in the OsNAC2 promoter (Table S3), including ABRELATERD1, which is known to be responsive to the plant hormone ABA (Narusaka et al ., ); WBBOXPCWRKY1, which is responsive to SA (Fang et al ., ), WRKY71OS, GT1CORE, and MYBCORE, which are responsive to environmental stimuli such as light and cold (Fang et al ., ; Kim et al ., ); and RYREPEATBNNAPA, which is related to seed‐specific expression (Fang et al ., ). The upstream factors involved in regulating OsNAC2 under salt stress, therefore, require further analysis.…”

Section: Discussionmentioning

confidence: 99%

OsNAC2 positively affects salt‐induced cell death and binds to the OsAP37 and OsCOX11 promoters

et al. 2018

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Plant development and adaptation to environmental stresses are intimately associated with programmed cell death (PCD). Although some of the mechanisms regulating PCD [e.g., accumulation of reactive oxygen species (ROS)] are common among responses to different abiotic stresses, the pathways mediating salt-induced PCD remain largely uncharacterized. Here we report that overexpression of OsNAC2, which encodes a plant-specific transcription factor, promotes salt-induced cell death accompanied by the loss of plasma membrane integrity, nuclear DNA fragmentation, and changes to caspase-like activity. In OsNAC2-knockdown lines, cell death was markedly decreased in response to severe salt stress. Additionally, OsNAC2 expression was enhanced in rice seedlings exposed to a high NaCl concentration. Moreover, the results of quantitative real-time PCR, chromatin immunoprecipitation, dual-luciferase, and yeast one-hybrid assays indicated that OsNAC2 targeted genes that encoded an ROS scavenger (OsCOX11) and a caspase-like protease (OsAP37). Furthermore, K -efflux channels (OsGORK and OsSKOR) were clearly activated by OsNAC2. Overall, our results suggested that OsNAC2 accelerates NaCl-induced PCD and provide new insights into the mechanisms that affect ROS accumulation, plant caspase-like activity, and K efflux.

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“…For example, cold stress and ABA treatment upregulated CsWRKY46, and the overexpression of CsWRKY46 increases the freezing and chilling tolerance in cucumber [20]. OsWRKY71 is highly upregulated in response to cold stress, and the overexpression of OsWRKY71 can increase the survival rate under cold treatment in rice [21].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of WRKY Genes and Their Response to Cold Stress in Coffea canephora

Dong

Yang

Zhang

et al. 2019

Forests

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WRKY transcription factors are known to play roles in diverse stress responses in plants. Low temperatures limit the geographic distribution of Coffea canephora Pierre ex A.Froehner. The WRKYs of C. canephora are still not well characterized, and the response of C. canephora WRKYs (CcWRKYs) under cold stress is still largely unknown. We identified 49 CcWRKYs from the C. canephora genome to gain insight into these mechanisms. These CcWRKYs were divided into three groups that were based on the conserved WRKY domains and zinc-finger structure. Gene expression analysis demonstrated that 14 CcWRKYs were induced during the cold acclimation stage, 17 CcWRKYs were preferentially upregulated by 4 °C treatment, and 12 CcWRKYs were downregulated by cold stress. Subsequently, we carried out a genome-wide analysis to predict 14,513 potential CcWRKY target genes in C. canephora. These isolated genes were involved in multiple biological processes, and most of them could be grouped by the response to stimulus. Among the putative CcWRKY target genes, 235 genes were categorized into response to the cold process, including carbohydrate metabolic, lipid metabolic, and photosynthesis process-related genes. Furthermore, the qRT-PCR and correlation analysis indicated that CcWRKY might control their putative targets that respond to cold stress. These results provide a basis for understanding the molecular mechanism for CcWRKY-mediated cold responses.

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Functional analysis of a cold-responsive rice WRKY gene, OsWRKY71

Cited by 102 publications

References 74 publications

MdWRKY40 promotes wounding‐induced anthocyanin biosynthesis in association with MdMYB1 and undergoes MdBT2‐mediated degradation

MdWRKY40 promotes wounding‐induced anthocyanin biosynthesis in association with MdMYB1 and undergoes MdBT2‐mediated degradation

OsNAC2 positively affects salt‐induced cell death and binds to the OsAP37 and OsCOX11 promoters

Genome-Wide Identification of WRKY Genes and Their Response to Cold Stress in Coffea canephora

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