Functional analysis of four upregulated carboxylesterase genes associated with fenpropathrin resistance in <i>Tetranychus cinnabarinus</i> (Boisduval)

Peng, Wei; Li, Jinhang; Liu, Xinyang; Nan, Can; Shi, Li; Zhang, Yichao; Li, Chuanzhen; He, Lin

doi:10.1002/ps.5109

Cited by 47 publications

(52 citation statements)

References 58 publications

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“…With the exception of acetylcholinesterases (AChEs), CCEs have only been indirectly implicated in resistance of T. urticae to various acaricides, e.g. bifenthrin and chlorfenapyr, and detailed molecular evidence has been lacking …”

Section: Introductionmentioning

confidence: 99%

“…bifenthrin and chlorfenapyr, and detailed molecular evidence has been lacking. [32][33][34][35] We report here on the discovery and association of a specific allele of CCE04 with spirodiclofen resistance in T. urticae, by analyzing gene expression, determining CCE04 copy number, and finally by studying recombinantly produced and purified CCE04 isoforms for their steady state enzyme kinetics and interaction with spirodiclofen.…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of an alternative allele of carboxyl/choline esterase 4 (CCE04) of Tetranychus urticae is associated with high levels of resistance to the keto‐enol acaricide spirodiclofen

Peng

Demaeght

Schutter

et al. 2019

Pest Management Science

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BACKGROUND Spirodiclofen is an acaricide that targets lipid biosynthesis by inhibiting acetyl‐coenzyme A carboxylase. Spirodiclofen resistance in spider mites has been previously documented and was associated with overexpression of CYP392E10, a cytochrome P450 mono‐oxygenase that metabolizes spirodiclofen. However, additional mechanisms have been suggested in several studies and a carboxyl/choline esterase gene, CCE04, was shown to be overexpressed in two genetically different strains, SR‐VP and SR‐TK, both exhibiting high spirodiclofen resistance levels. RESULTS We identified two different CCE04 alleles in both resistant strains, CCE04SR‐VP and CCE04London, with CCE04SR‐VP being highly overexpressed. Isoelectric focusing analysis confirmed the overexpression of a single esterase isozyme, while copy number and random fragment length polymorphism analysis revealed that CCE04SR‐VP overexpression was more likely due to selection for the CCE04SR‐VP allele rather than gene amplification. Both CCE04 alleles were functionally expressed using the Pichia expression system. Functional enzyme assays revealed only limited kinetic differences between CCE04 isoforms for model substrates. In addition, inhibition/competition experiments with spirodiclofen suggested a similar interaction with both enzymes, whereas its active metabolite, spirodiclofen enol, did not inhibit enzyme activity. CONCLUSION Our study suggests that selection with spirodiclofen results in enrichment of a specific allele of CCE04 (CCE04SR‐VP) in two genetically independent strains, which is highly overexpressed. Based on kinetic enzyme data, however, quantitative rather than qualitative differences between CCE04SR‐VP and CCE04London seem more likely to be involved in resistance. Our findings are discussed in the light of a possible spirodiclofen resistance mechanism, with sequestration of spirodiclofen by CCE04SR‐VP being a likely hypothesis. © 2019 Society of Chemical Industry

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Overexpression of an alternative allele of carboxyl/choline esterase 4 (CCE04) of Tetranychus urticae is associated with high levels of resistance to the keto‐enol acaricide spirodiclofen

Peng

Demaeght

Schutter

et al. 2019

Pest Management Science

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“…Moreover, it is worth mentioning that we could not ignore the contributions of other upregulated GST genes since six overexpressed GST genes were detected in CyR strain. The collaborative contribution against resistance from multiple up‐regulated genes had been demonstrated in resistant insects and mites . Nevertheless, the superiority of TcGSTm02 in cyflumetofen‐resistant T. cinnabarinus is interesting and important.…”

Section: Discussionmentioning

confidence: 99%

“…The collaborative contribution against resistance from multiple up-regulated genes had been demonstrated in resistant insects and mites. [37][38][39] Nevertheless, the superiority of TcGSTm02 in cyflumetofen-resistant T. cinnabarinus is interesting and important.…”

Section: Discussionmentioning

confidence: 99%

Stability of cyflumetofen resistance in Tetranychus cinnabarinus and its correlation with glutathione‐S‐transferase gene expression

Feng

Yang

Wen

et al. 2019

Pest Management Science

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BACKGROUND Cyflumetofen is an outstanding acaricide with a novel mode of action. Tetranychus cinnabarinus, an important agricultural pest, is notorious for developing resistance to most classes of acaricides rapidly and results in enormous loss for the economy. Our previous study had pointed out glutathione S‐transferase (GSTs) significantly contributed to the cyflumetofen‐resistance formation in T. cinnabarinus, but the more specific mechanism needed to be further investigated. RESULTS The unstable resistance was observed in cyflumetofen‐resistant strain (CyR)under acaricide‐free condition. The activity of GSTs increased along with the development of resistance. The expressions of 13 GST genes were detected in CyR and susceptible strain (SS), of which six genes were overexpressed in CyR and the TcGSTm02 was selected as the representative for functional study. The expression of TcGSTm02 changed along with the resistant level of CyR with the same trend. Recombinant protein of TcGSTm02 with high activity was successfully obtained by E. coli expression system, whose activity could be inhibited by cyflumetofen (IC50 = 0.23 mM). Recombinant TcGSTm02 could effectively decompose cyflumetofen, and catalyze GS− to conjugate with cyflumetofen. CONCLUSION All clues confirmed that GSTs strongly associated with cyflumetofen‐resistance and a representative gene, TcGSTm02, showed function on contributing the evolution of cyflumetofen‐resistance in T. cinnabarinus. © 2019 Society of Chemical Industry

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“…The permanent darkness and the low oxygen level do not cause problems either. In most of their species resistance had developed to the special acaricides as well [3][4][5]. In case of a record-breaking mass extinction due to the destruction of the environment by mankind, they will have representatives among the survivors, since many of their groups had survived the most devastating Permian-Triassic extinction to date almost without any change.…”

Section: The Scientific Importance Of Mitesmentioning

confidence: 99%

Prologue: Scientific and Societal Importance of Mites and Acarology From the Viewpoint of International Publication

Hufnágel¹,

Mics²,

Homoródi³

2020

Pests Control and Acarology

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Functional analysis of four upregulated carboxylesterase genes associated with fenpropathrin resistance in Tetranychus cinnabarinus (Boisduval)

Cited by 47 publications

References 58 publications

Overexpression of an alternative allele of carboxyl/choline esterase 4 (CCE04) of Tetranychus urticae is associated with high levels of resistance to the keto‐enol acaricide spirodiclofen

Overexpression of an alternative allele of carboxyl/choline esterase 4 (CCE04) of Tetranychus urticae is associated with high levels of resistance to the keto‐enol acaricide spirodiclofen

Stability of cyflumetofen resistance in Tetranychus cinnabarinus and its correlation with glutathione‐S‐transferase gene expression

Prologue: Scientific and Societal Importance of Mites and Acarology From the Viewpoint of International Publication

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