Functional analysis of <i>Dicer‐2</i> gene in <i>Bombyx mori</i> resistance to BmNPV virus

Li, Taohong; Xia, Yuchen; Xu, Xinyue; Wei, Guoqing; Wang, Lei

doi:10.1002/arch.21724

Cited by 16 publications

(18 citation statements)

References 41 publications

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“…Although DNA viruses do not replicate through a dsRNA intermediate, overlapping transcripts produced by compact genomes lead to significant amounts of dsRNA (13). Consistently, vsiRNAs can be detected during infections with baculoviruses and other insect DNA viruses (3,(14)(15)(16), and viral infections are improved in RNAi-defective mutants for these cases (17)(18)(19)(20).…”

Section: Introductionmentioning

confidence: 85%

BmNPV p35 Reduces the Accumulation of Virus-Derived siRNAs and Hinders the Function of siRNAs to Facilitate Viral Infection

et al. 2022

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Antiviral immunity involves various mechanisms and responses, including the RNA interference (RNAi) pathway. During long-term coevolution, viruses have gained the ability to evade this defense by encoding viral suppressors of RNAi (VSRs). It was reported that p35 of baculovirus can inhibit cellular small interference RNA (siRNA) pathway; however, the molecular mechanisms underlying p35 as a VSR remain largely unclear. Here, we showed that p35 of Bombyx mori nucleopolyhedrovirus (BmNPV) reduces the accumulation of virus-derived siRNAs (vsiRNAs) mapped to a particular region in the viral genome, leading to an increased expression of the essential genes in this region, and revealed that p35 disrupts the function of siRNAs by preventing them from loading into Argonaute-2 (Ago2). This repressive effect on the cellular siRNA pathway enhances the replication of BmNPV. Thus, our findings illustrate for the first time the inhibitory mechanism of a baculovirus VSR and how this effect influences viral infection.

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Section: Introductionmentioning

confidence: 85%

BmNPV p35 Reduces the Accumulation of Virus-Derived siRNAs and Hinders the Function of siRNAs to Facilitate Viral Infection

et al. 2022

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“…BmActin A3 (GenBank No. U49854) was used as an internal reference gene (Li et al, 2020). The procedure of RT-qPCR was as follows: the first cycle was 95℃ with 30 s, followed by 40 cycles (95℃, 5 s; 58℃, 15 s; and 72℃, 10 s).…”

Section: Expression Analysis Of Bmhr96 Gene In Different Tissue and S...mentioning

confidence: 99%

Functional analysis of nuclear receptor HR96 gene in Bombyx mori exposed to phoxim

Liu

et al. 2022

Arch Insect Biochem Physiol

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The nuclear receptor (NRs) gene family functions as liganddependent transcription factors in a variety of animals, which participates in a variety of biological processes, such as cell differentiation, metabolic regulation, reproduction, development, insect metamorphosis. In this study, a nuclear receptor HR96 gene in silkworm Bombyx mori (BmHR96) was identified, and the responses of BmHR96 gene to 20-hydroxyecdysone (20E), three insecticides, and two disinfectants were analyzed and its function in phoxim exposure was explored. Quantitative real-time polymerase chain reaction indicated that the expression of BmHR96 mRNA was the highest in ovary of 5th instar Day 3 silkworm larvae and in silk gland of the wandering stage. The expression patterns of BmHR96 gene in ovary, head, testis, and midgut of different stages were different. After injecting 20E into B. mori, the expression of BmHR96 mRNA had no significant difference compared with control. Three insecticides and two disinfectants were used to treat B. mori, respectively, and it was found that they had different influence patterns on the expression level of BmHR96. siRNA of BmHR96 was injected into silkworm larvae and the expression of BmHR96 was decreased significantly after injecting 72 h. After silencing of BmHR96, B. mori was fed with phoxim-treated leaves. The results showed that the mortality of B. mori after silencing of BmHR96 was significantly higher than the control. Our results indicated

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“…The siRNA pathway is composed of three major proteins: Dicer-2 (Dcr2), double-stranded RNA binding protein (dsRBP) R2D2, and Argonaute-2 (Ago2). In Bombix mori , Dcr2 is highly expressed in the hemocytes, and expression levels have been further shown to increase in these cells after nucleopolyhedroviral (BmNPV) infection [ 60 ]. Knocking down Dcr2 also increases BmNPV viral titers.…”

Section: Antiviral Immune Pathway In Hemocytesmentioning

confidence: 99%

The Role of Mosquito Hemocytes in Viral Infections

Cardoso-Jaime

Tikhe

Dong

et al. 2022

Viruses

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Insect hemocytes are the only immune cells that can mount a humoral and cellular immune response. Despite the critical involvement of hemocytes in immune responses against bacteria, fungi, and parasites in mosquitoes, our understanding of their antiviral potential is still limited. It has been shown that hemocytes express humoral factors such as TEP1, PPO, and certain antimicrobial peptides that are known to restrict viral infections. Insect hemocytes also harbor the major immune pathways, such as JAK/STAT, TOLL, IMD, and RNAi, which are critical for the control of viral infection. Recent research has indicated a role for hemocytes in the regulation of viral infection through RNA interference and autophagy; however, the specific mechanism by which this regulation occurs remains uncharacterized. Conversely, some studies have suggested that hemocytes act as agonists of arboviral infection because they lack basal lamina and circulate throughout the whole mosquito, likely facilitating viral dissemination to other tissues such as salivary glands. In addition, hemocytes produce arbovirus agonist factors such as lectins, which enhance viral infection. Here, we summarize our current understanding of hemocytes’ involvement in viral infections.

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Functional analysis of Dicer‐2 gene in Bombyx mori resistance to BmNPV virus

Cited by 16 publications

References 41 publications

BmNPV p35 Reduces the Accumulation of Virus-Derived siRNAs and Hinders the Function of siRNAs to Facilitate Viral Infection

BmNPV p35 Reduces the Accumulation of Virus-Derived siRNAs and Hinders the Function of siRNAs to Facilitate Viral Infection

Functional analysis of nuclear receptor HR96 gene in Bombyx mori exposed to phoxim

The Role of Mosquito Hemocytes in Viral Infections

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