Functional analysis of inhibitor of apoptosis 1 of the silkworm Bombyx mori

Hamajima, Rina; Iwamoto, Asako; Tomizaki, Moe; Suganuma, Ikue; Kitaguchi, Koji; Kobayashi, Michihiro; Yamada, Hayato; Ikeda, Motoko

doi:10.1016/j.ibmb.2016.10.012

Cited by 23 publications

(16 citation statements)

References 62 publications

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“…IAP is a family of antiapoptotic proteins and plays important roles in both apoptosis and innate immunity (Leu et al, 2008). Both BmIAP and baculoviruses encoding IAP had an inhibitory effect on apoptosis in insects (Hamajima et al, 2016;Ikeda et al, 2011). A recent study reported that the Bmiap gene might be hijacked by BmNPV iap genes during BmNPV infection owing to the direct interaction of BmNPV IAP1 and IAP2 with BmIAP in silkworm cells (Chen et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of heat shock protein 90 suppresses Bombyx mori nucleopolyhedrovirus replication in B. mori

Shang

Huang

et al. 2019

Insect Molecular Biology

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Heat shock protein 90 (Hsp90) plays a very important role in facilitating the replication of many viruses. Until now, little has been known about the role of Hsp90 in Bombyx mori virus infection. In this study, we explored the role of BmHsp90 in B. mori nucleopolyhedrovirus (BmNPV) replication. We found that BmHsp90 inhibition by geldanamycin (GA) significantly reduced the BmNPV titre, the protein expression level of BmNPV nucleocapsid protein 39 (VP39) and the transcript level of BmNPV genes. Silencing the hsp90 gene in BmN cells by small interfering RNA suppressed BmNPV replication whereas overexpression of hsp90 promoted the replication of BmNPV. After inhibition of Hsp90, the expression of three key genes [signal transducing activator of transcription (stat), suppressor of cytokine signalling protein 2 (socs2), socs6] involved in the Janus kinase/STAT pathway significantly changed, with up‐regulation of stat and down‐regulation of socs2 and socs6. In addition, the expression of two antiapoptosis genes, BmNPV inhibitor of apoptosis protein1 (BmNPV‐iap1) and Bmiap2, was greatly decreased in GA‐treated cells, whereas their expression was significantly increased in hsp90‐overexpressed silkworm larvae. Our results indicated that inhibition of Hsp90 can suppress BmNPV proliferation in B. mori. Our findings may provide new clues to elucidate the molecular mechanisms of silkworm–virus interactions.

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Section: Discussionmentioning

confidence: 99%

Inhibition of heat shock protein 90 suppresses Bombyx mori nucleopolyhedrovirus replication in B. mori

Shang

Huang

et al. 2019

Insect Molecular Biology

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“…Bombyx mori IAPs (BmIAPs) can inhibit the apoptosis of silkworm cells and inhibit silkworm apoptotic proteins (Yi et al, 2017). Hamajima et al (2016) demonstrated that BmIAP1 acts as a key negative regulator of apoptosis by interfering with caspase cascades in cells. Based on the available genomic data, 52 apoptosis-related candidate genes have been identified in silkworms (Courtiade et al, 2011), including five caspases (Zhang et al, 2010).…”

Section: Other Influencing Factors Related To Apoptosismentioning

confidence: 99%

Research progress on the immune mechanism of the silkworm Bombyx mori

et al. 2018

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The silkworm Bombyx mori L., representing an important economic insect and one of the best models for studying insect immunity, possesses an efficient and sophisticated innate immune system against invasive microorganisms. The innate immune system basically includes humoural immunity and cellular immunity. The humoural immunity, which functions via molecules including humoural factors, lysozymes, phenoloxidase, hemolin, lectins and, in particular, antimicrobial peptides, plays a central role in eliminating the invading pathogens. The cellular immunity is primarily carried out and mediated by plasmatocytes and granular cells of haemocytes in the haemolymph, usually followed by melanization. Additionally, apoptosis, a primary viral defence for insects lacking adaptive immunity, comprises an important part of the silkworm immune system. Currently, there is still the lack of a comprehensive and systematic understanding of the molecular mechanisms of silkworm immunity. We review the latest research progress on silkworm immune mechanisms, including phenoloxidase‐dependent melanization and apoptosis, which is conducive to improving our understanding of the silkworm immune mechanism, clarifying the relationship of various immune mechanisms, and also providing a theoretical basis and reference for the future research of insect immunity.

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“…Recently, Op‐IAP3 was reported to inhibit virus‐induced apoptosis by interacting with the Spodoptera frugiperda iap ( SfIAP ) gene (Byers et al ., ). In Bombyx mori , an important model organism for Lepidoptera, we previously identified four IAP family members (Zhang et al ., ), among which the survivin gene was reported to play a role in the cell apoptosis induced by viruses (Tang et al ., ), and the iap 1 gene was involved in the negative regulation of apoptosis in BM‐N cells (Hamajima et al ., ). In addition, two iap genes were identified in the genome of B. mori nucleopolyhedrovirus (BmNPV) (Gomi et al ., ; Cheng et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Evolutionary and functional analyses of the interaction between the Bombyx mori inhibitor of apoptosis (IAP) and nucleopolyhedrovirus IAPs

et al. 2019

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As an important insect immune response, apoptosis plays a critical role in the interaction between baculoviruses and insect hosts. Previous reports have identified inhibitor of apoptosis (IAP) proteins in both insects and baculoviruses, but the relationship between these proteins is still not clearly understood. Here, we found that insect IAP proteins were clustered with baculovirus IAP3, suggesting that the baculovirus iap3 gene might be derived from the Lepidoptera or Diptera. We demonstrated that Bombyx mori inhibitor of apoptosis (Bmiap) gene had an inhibitory effect on apoptosis in silkworm cells. Further analysis of the effects of Bmiap genes on the proliferation of B. mori nucleopolyhedrovirus (BmNPV) showed that both the Bmiap and BmNPV iap genes increased BmNPV proliferation after BmNPV infected silkworm cells. Our results also indicated that BmNPV IAP1 and IAP2 directly interacted with BmIAP in silkworm cells, implying that the Bmiap gene might be hijacked by BmNPV iap genes during BmNPV infection. Taken together, our results provide important insights into the functional relationships of iap genes, and improve our knowledge of apoptosis in baculoviruses and insect hosts.

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Functional analysis of inhibitor of apoptosis 1 of the silkworm Bombyx mori

Cited by 23 publications

References 62 publications

Inhibition of heat shock protein 90 suppresses Bombyx mori nucleopolyhedrovirus replication in B. mori

Inhibition of heat shock protein 90 suppresses Bombyx mori nucleopolyhedrovirus replication in B. mori

Research progress on the immune mechanism of the silkworm Bombyx mori

Evolutionary and functional analyses of the interaction between the Bombyx mori inhibitor of apoptosis (IAP) and nucleopolyhedrovirus IAPs

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