Functional Analysis of Lactobacillus rhamnosus GG Pili in Relation to Adhesion and Immunomodulatory Interactions with Intestinal Epithelial Cells

Lebeer, Sarah; Claes, Ingmar; Tytgat, Hanne L. P.; Verhoeven, Tine; Marien, Eyra; Ossowski, Ingemar von; Reunanen, Justus; Palva, Airi; Vos, Willem M. de; Keersmaecker, Sigrid C. J. De; Vanderleyden, Jos

doi:10.1128/aem.06192-11

Cited by 288 publications

(319 citation statements)

References 44 publications

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“…20,21 Nonpilus adhesins of several bacteria also play pivotal roles in adherence to the host and its immune response. In pathogenic bacteria, trimeric autotransporter adhesins 22 and adhesins belonging to a protein family termed microbial surface components recognizing adhesive matrix molecules (MSCRAMMs) 23 were found as virulence factors in Gram-negative and -positive bacteria, respectively.…”

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confidence: 99%

Is it feasible to control pathogen infection by competitive binding of probiotics to the host?

Fukuda

2017

Virulence

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confidence: 99%

Is it feasible to control pathogen infection by competitive binding of probiotics to the host?

Fukuda

2017

Virulence

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“…The recent discovery of mucus-binding pili constituted a milestone in understanding how L. rhamnosus strain GG interacts with and signals its host (4,9,21,23). The genetic organization of L. rhamnosus GG pilus cluster is quite similar to some other piliated Gram-positive bacteria, such as E. faecalis (11).…”

Section: Discussionmentioning

confidence: 99%

“…L. rhamnosus GG 2 is globally marketed in probiotic products and has been among the best studied lactic acid bacteria (5)(6)(7)(8). In GG, the discovery that mucus-binding pili signal the intestinal cells has generated new insights into the mechanisms involved in host interactions and underlines the potential contribution of pili in probiotic response (9). However, it is not clear what the signaling mechanisms and molecular components are that are involved in this response.…”

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confidence: 99%

Functional Identification of Conserved Residues Involved in Lactobacillus rhamnosus Strain GG Sortase Specificity and Pilus Biogenesis

Douillard

Rasinkangas

Ossowski

et al. 2014

Journal of Biological Chemistry

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Background: Lactobacillus rhamnosus GG produces pili assembled and anchored by two distinct sortases, SrtC and SrtA, respectively. Results: Residue substitution within the triple glycine (TG) motif of pilin proteins impacts sortase-mediated polymerization. Conclusion:The TG motif determines sortase specificity during pilus biogenesis in GG. Significance: These results help explain the signaling involved in the assembly and anchoring of sortase-dependent pili.

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“…Previous studies have investigated the potential of EPSs as antiproliferative or anticytoxic agents with intestinal epithelial cells (Ruas-Madiedo et al, 2010;Liu et al, 2011), but, the immunomodulatory effects of EPSs on these cells has largely been neglected in the literature. However, Lebeer et al (2012), as part of a much larger investigation, have reported that EPSs isolated from the known probiotic organism Lactobacillus rhamnosus GG had no significant effect on IL-8 mRNA expression in Caco-2 cells. Additionally, a recent review article presented preliminary data in which co-culture with EPS-producing strains of bifidobacteria differentially modulated the secretion of inflammatory cytokines, including IL-8 and IL-6, in the Caco-2 intestinal epithelial cell line (Hidalgo-Cantabrana et al, 2012).…”

Section: Exopolysaccharidesmentioning

confidence: 99%

Exploring the immunomodulatory potential of microbial-associated molecular patterns derived from the enteric bacterial microbiota

Patten

Collett

2013

Microbiology

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The human intestinal lumen represents one of the most densely populated microbial niches in the biological world and, as a result, the intestinal innate immune system exists in a constant state of stimulation. A key component in the innate defence system is the intestinal epithelial layer, which acts not only as a physical barrier, but also as an immune sensor. The expression of pattern recognition receptors, such as Toll-like receptors, in epithelial cells allows innate recognition of a wide range of highly conserved bacterial moieties, termed microbial-associated molecular patterns (MAMPs), from both pathogenic and non-pathogenic bacteria. To date, studies of epithelial immunity have largely concentrated on inflammatory pathogenic antigens; however, this review discusses the major types of MAMPs likely to be produced by the enteric bacterial microbiota and, using data from in vitro studies, animal model systems and clinical observations, speculates on their immunomodulatory potential. IntroductionThe intestine represents the body's largest mucosal surface, with the adult human intestine estimated to cover an area of about 250 m 2 (Artis, 2008). The highly folded luminal surface of the intestinal wall significantly increases absorption efficiency and, as such, is the largest surface area of the body exposed to the environment and its high microbial load (DeSesso & Jacobson, 2001). Humans have co-evolved with indigenous microbial populations, termed microbiota, which inhabit various niches of the body (Hooper et al., 2012) and the adult intestine is one of the most densely populated microbial habitations in the biological world (Artis, 2008). The enteric microbiota predominantly consists of bacteria, with estimated populations of~10 14 bacteria, with up to 500 species represented (Gill et al., 2006;Guarner & Malagelada, 2003); however, methanogenic archaea, eukaryotes (yeasts) and viruses (mainly bacteriophages) are also present (Lozupone et al., 2012). Due to the vast expanse of intestinal tissue exposed to the microbiota, the local innate immune system is in a constant state of stimulation, and a chronic, low-level proinflammatory response is characteristic of enteric immune homeostasis (Macpherson & Harris, 2004;Artis, 2008).The intestinal epithelium plays an active role in innate immunity, and pattern recognition receptors (PRRs) are utilized to detect the presence of bacteria and their associated antigens. PRRs are germline-encoded, sensory molecules which recognize a range of highly conserved bacterial motifs, termed 'pathogen-associated molecular patterns' (PAMPs) (Medzhitov, 2001). However, the ability of PRRs to recognize these bacterial moieties is not limited to just pathogens, and so the term 'microbial-associated molecular patterns' (MAMPs) may be more accurate (Medzhitov, 2001; Sanderson & Walker, 2007) and will be used throughout this review. Epithelium-associated, enteric immune cells, such as macrophages, dendritic cells, T-cells and B-cells, differentially express two major groups of PRRs, th...

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Functional Analysis of Lactobacillus rhamnosus GG Pili in Relation to Adhesion and Immunomodulatory Interactions with Intestinal Epithelial Cells

Abstract: Lactobacillus rhamnosus GG, a probiotic with good survival capacity in the human gut, has well-documented adhesion properties and health effects. Recently, spaCBA-encoded pili that bind to human intestinal mucus were identified on its cell surface.

Cited by 288 publications

References 44 publications

Is it feasible to control pathogen infection by competitive binding of probiotics to the host?

Is it feasible to control pathogen infection by competitive binding of probiotics to the host?

Functional Identification of Conserved Residues Involved in Lactobacillus rhamnosus Strain GG Sortase Specificity and Pilus Biogenesis

Exploring the immunomodulatory potential of microbial-associated molecular patterns derived from the enteric bacterial microbiota

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