Functional analysis of polyketide synthase genes in the biocontrol fungus Clonostachys rosea

Fatema, Umma; Broberg, Anders; Jensen, Dan Funck; Karlsson, Magnus; Dubey, Mukesh

doi:10.1038/s41598-018-33391-1

Cited by 54 publications

(95 citation statements)

References 73 publications

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“…It is also possible that some of the highly up-regulated genes, particularly PKSs, are involved in fungal development and/or protection as well. For example, two up-regulated PKSs in C. rosea IK726 upon interaction with F. graminearum were previously identified; however deletion of one led to pigmentation loss, while deletion of the other reduced the antagonistic activity of C. rosea IK726 against F. graminearum (Fatema, 2017 ). Secondary metabolism-related secondary metabolites have also been associated with fungal sclerotial development (Calvo and Cary, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Profiling of the Transcriptomic Responses of Clonostachys rosea Upon Treatment With Fusarium graminearum Secretome

et al. 2018

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Clonostachys rosea strain ACM941 is a fungal bio-control agent patented against the causative agent of Fusarium Head Blight, Fusarium graminearum. Although the molecular details remain enigmatic, previous studies have suggested that C. rosea may secrete F. graminearum growth inhibitors. Further toward this, experiments described herein show that induction of C. rosea cultures by the addition of an aliquot of F. graminearum(Fg)-spent media (including macroconidia), yield C. rosea (Cr)-spent media that elicited higher anti-F. graminearum activity than either control or deoxynivalenol (DON)-induced Cr-spent media. To gain additional insight into the genetic and metabolic factors modulating this interaction, transcriptomic (RNAseq) profiles of C. rosea in response to DON and Fg-spent media treatment, were developed. This analysis revealed 24,112 C. rosea unigenes, of which 5,605 and 6,285 were differentially regulated by DON and F-spent media, respectively. More than half of these unigenes were up-regulated, with annotations, most notably in the Fg-spent media treatment data, suggesting enhancement of polyketide (PK) and non-ribosomal peptide (NRP) secondary metabolite precursor synthesis, and PK/NRP-like synthases. Four ABC transporters were also up-regulated in response to Fg-spent media. Further analysis showed that the PK and NRP-like synthases belong to three gene clusters that also include ABC transporters, and other genes known to tailor secondary metabolite biosynthesis. The RNAseq data was further validated using quantitative RT-qPCR. Taken together, these results show that C. rosea responds to the presence of Fg-spent media (and to a lesser extent, DON-alone) by up-regulating unique aspects of its secondary metabolism-related genetic repertoire. The identities and roles of C. rosea secondary metabolites produced by the targeted gene clusters are now under investigation.

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Section: Discussionmentioning

confidence: 99%

Profiling of the Transcriptomic Responses of Clonostachys rosea Upon Treatment With Fusarium graminearum Secretome

et al. 2018

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“…As an excellent biological agent, C. rosea plays crucial roles in the biological control of many kinds of pathogens. To date, C. rosea has been reported to act against numerous fungal plant pathogens including Alternaria dauci, A. radicina, B. cinerea, B. aclada, Bipolaris sorokiniana, Drechslera teres, F. graminearum, F. verticillioides, F. The biological control mechanism of C. rosea against pathogens is primarily attributed to the secretion of CWDEs, and the production of secondary metabolites such as antibiotics and toxins, and the induction of plant resistance (Chatterton and Punja 2009;Fatema et al 2018). Although the constituents of fungal cell walls are complex, they are mainly composed of chitin, glucan and glycoproteins (Bowman and Free 2006).…”

Section: Role Of C Rosea In Biological Controlmentioning

confidence: 99%

Biology and applications of Clonostachys rosea

et al. 2020

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Clonostachys rosea is a promising saprophytic filamentous fungus that belongs to phylum Ascomycota. Clonostachys rosea is widespread around the world and exists in many kinds of habitats, with the highest frequency in soil. As an excellent mycoparasite, C. rosea exhibits strong biological control ability against numerous fungal plant pathogens, nematodes and insects. These behaviours are based on the activation of multiple mechanisms such as secreted cell-walldegrading enzymes, production of antifungal secondary metabolites and induction of plant defence systems. Besides having significant biocontrol activity, C. rosea also functions in the biodegradation of plastic waste, biotransformation of bioactive compounds, as a bioenergy sources and in fermentation. This mini review summarizes information about the biology and various applications of C. rosea and expands on its possible uses.

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“…Fatema et al (2018) demonstrated that two polyketide synthase genes, PKS22 and PKS29, play important roles in the synthesis of antifungal agents, clonorosein A-D that are effective against B. cinerea. Dubey et al (2016) found that the ABC transporter gene ABCG29 is involved in fungal adaptation to oxidative stress in the early stages of mycelial development and biocontrol of B. cinerea and Fusarium graminearum. Sun et al (2019a) indicated that the heat shock protein 70 gene, crhsp, had a remarkable effect on C. rosea morphological characteristics and significantly reduced its ability to parasitize S. sclerotiorum sclerotia.…”

Section: Introductionmentioning

confidence: 99%

“…This species has great potential for controlling various plant fungal diseases and promoting crop growth ( Atanasova et al, 2018 ), and functional genes in particular have attracted much attention. Fatema et al (2018) demonstrated that two polyketide synthase genes, PKS22 and PKS29 , play important roles in the synthesis of antifungal agents, clonorosein A–D that are effective against B. cinerea . Dubey et al (2016) found that the ABC transporter gene ABCG29 is involved in fungal adaptation to oxidative stress in the early stages of mycelial development and biocontrol of B. cinerea and Fusarium graminearum .…”

Section: Introductionmentioning

confidence: 99%

Cell Wall Biogenesis Protein Phosphatase CrSsd1 Is Required for Conidiation, Cell Wall Integrity, and Mycoparasitism in Clonostachys rosea

Jiang

Hasan

et al. 2020

Front. Microbiol.

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Cell wall biogenesis protein phosphatases play important roles in various cellular processes in fungi. However, their functions in the widely distributed mycoparasitic fungus Clonostachys rosea remain unclear, as do their potential for controlling plant fungal diseases. Herein, the function of cell wall biogenesis protein phosphatase CrSsd1 in C. rosea 67-1 was investigated using gene disruption and complementation approaches. The gene-deficient mutant ΔCrSsd1 exhibited much lower conidiation, hyphal growth, mycoparasitic ability, and biocontrol efficacy than the wild-type (WT) strain, and it was more sensitive to sorbitol and Congo red. The results indicate that CrSsd1 is involved in fungal conidiation, osmotic stress adaptation, cell wall integrity, and mycoparasitism in C. rosea.

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Functional analysis of polyketide synthase genes in the biocontrol fungus Clonostachys rosea

Cited by 54 publications

References 73 publications

Profiling of the Transcriptomic Responses of Clonostachys rosea Upon Treatment With Fusarium graminearum Secretome

Profiling of the Transcriptomic Responses of Clonostachys rosea Upon Treatment With Fusarium graminearum Secretome

Biology and applications of Clonostachys rosea

Cell Wall Biogenesis Protein Phosphatase CrSsd1 Is Required for Conidiation, Cell Wall Integrity, and Mycoparasitism in Clonostachys rosea

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