Functional Analysis of six novel ORFs on the left arm of Chromosome XII ofSaccharomyces cerevisiae reveals three of them responding to S-starvation

Zhang, Nianshu; Merlotti, Cristina; Wu, Jian; Ismail, Thamir M.; El-Moghazy, Abdel Nasser; Khan, Shakeel Ahmed; Butt, Amna; Gardner, David C.; Sims, Paul F. G.; Oliver, Stephen G.

doi:10.1002/1097-0061(20010315)18:4<325::aid-yea669>3.0.co;2-k

Cited by 13 publications

(8 citation statements)

References 28 publications

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“…In S. cerevisiae, JLP1 encodes a sulfonate/alpha-ketoglutarate dioxygenase (36), involved in sulfonate catabolism, which is overexpressed under sulfur starvation (37). In Y. lipolytica, six orthologs of the JLP1 gene product belonging to the TauD protein family (Pfam number Pf02668) have been identified.…”

Section: Discussionmentioning

confidence: 99%

New Insights into Sulfur Metabolism in Yeasts as Revealed by Studies of Yarrowia lipolytica

Hébert

Forquin-Gomez

Roux

et al. 2013

Appl Environ Microbiol

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Yarrowia lipolytica, located at the frontier of hemiascomycetous yeasts and fungi, is an excellent candidate for studies of metabolism evolution. This yeast, widely recognized for its technological applications, in particular produces volatile sulfur compounds (VSCs) that fully contribute to the flavor of smear cheese. We report here a relevant global vision of sulfur metabolism in Y. lipolytica based on a comparison between high-and low-sulfur source supplies (sulfate, methionine, or cystine) by combined approaches (transcriptomics, metabolite profiling, and VSC analysis). The strongest repression of the sulfate assimilation pathway was observed in the case of high methionine supply, together with a large accumulation of sulfur intermediates. A high sulfate supply seems to provoke considerable cellular stress via sulfite production, resulting in a decrease of the availability of the glutathione pathway's sulfur intermediates. The most limited effect was observed for the cystine supply, suggesting that the intracellular cysteine level is more controlled than that of methionine and sulfate. Using a combination of metabolomic profiling and genetic experiments, we revealed taurine and hypotaurine metabolism in yeast for the first time. On the basis of a phylogenetic study, we then demonstrated that this pathway was lost by some of the hemiascomycetous yeasts during evolution.

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Section: Discussionmentioning

confidence: 99%

New Insights into Sulfur Metabolism in Yeasts as Revealed by Studies of Yarrowia lipolytica

Hébert

Forquin-Gomez

Roux

et al. 2013

Appl Environ Microbiol

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“…alternative sulfur sources [38,39]. We therefore hypothesized that Hsu1p could function under sulfur limitation conditions in conjunction with other enzymes that enable the usage of alternative sulfur sources, such as H 2 S. To explore this possibility, we analyzed the transcriptomic data acquired from a prototrophic yeast strain grown under sulfur limitation [40] together with genes identified as part of alternative sulfur usage regulons [39].…”

Section: Plos Biologymentioning

confidence: 99%

Inorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy

Heineike

Hartl

et al. 2022

PLoS Biol

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The assimilation, incorporation, and metabolism of sulfur is a fundamental process across all domains of life, yet how cells deal with varying sulfur availability is not well understood. We studied an unresolved conundrum of sulfur fixation in yeast, in which organosulfur auxotrophy caused by deletion of the homocysteine synthase Met17p is overcome when cells are inoculated at high cell density. In combining the use of self-establishing metabolically cooperating (SeMeCo) communities with proteomic, genetic, and biochemical approaches, we discovered an uncharacterized gene product YLL058Wp, herein named Hydrogen Sulfide Utilizing-1 (HSU1). Hsu1p acts as a homocysteine synthase and allows the cells to substitute for Met17p by reassimilating hydrosulfide ions leaked from met17Δ cells into O-acetyl-homoserine and forming homocysteine. Our results show that cells can cooperate to achieve sulfur fixation, indicating that the collective properties of microbial communities facilitate their basic metabolic capacity to overcome sulfur limitation.

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“…Transcriptomic data recorded by Oliver and colleagues (Boer et al, 2003; Zhang et al, 2001) show that Hsu1p is induced during sulfur limitation, although how the expression of this additional homocysteine synthase can contribute to a growth advantage remains unclear. We observed that the accumulation of H 2 S overcomes the loss of Met17p in the presence of Hsu1p, either directly provided or generated as a result of sulfide overflow in high density cultures.…”

Section: Discussionmentioning

confidence: 99%

Inorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy

Heineike

Hartl

et al. 2022

Preprint

View full text Add to dashboard Cite

The assimilation, incorporation, and metabolism of sulfur is a fundamental process across all domains of life, yet how cells deal with varying sulfur availability is not well understood. We studied an unresolved conundrum of sulfur fixation in yeast, in which an organosulfur-auxotrophy caused by deletion of homocysteine synthase Met17p is overcome when cells are inoculated at high cell density. We discovered that an uncharacterized gene YLL058Wp, herein named Hydrogen sulfide utilizing-1 (HSU1), acts as a homocysteine synthase and allows the cells to substitute for Met17p by re-assimilating hydrosulfide ions leaked from met17Δ cells into O-acetyl-homoserine and forming homocysteine. Our results show that cells can cooperate to achieve sulfur fixation, indicating that the collective properties of microbial communities facilitate their basic metabolic capacity.

show abstract

Functional Analysis of six novel ORFs on the left arm of Chromosome XII ofSaccharomyces cerevisiae reveals three of them responding to S-starvation

Cited by 13 publications

References 28 publications

New Insights into Sulfur Metabolism in Yeasts as Revealed by Studies of Yarrowia lipolytica

New Insights into Sulfur Metabolism in Yeasts as Revealed by Studies of Yarrowia lipolytica

Inorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy

Inorganic sulfur fixation via a new homocysteine synthase allows yeast cells to cooperatively compensate for methionine auxotrophy

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