Functional analysis of tomato rhamnogalacturonan lyase gene Solyc11g011300 during fruit development and ripening

Ochoa-Jiménez, Verónica Alhelí; Berúmen-Varela, Guillermo; Burgara-Estrella, Alexel; Orozco-Avitia, Jesús-Antonio; Ojeda-Contreras, Ángel-Javier; Trillo-Hernández, Eduardo-Antonio; Rivera-Domínguez, Marisela; Troncoso‐Rojas, Rosalba; Báez­-Sañudo, Reginaldo; Datsenka, Tatsiana; Handa, Avtar K.; Tiznado-Hernández, Martín-Ernesto

doi:10.1016/j.jplph.2018.09.001

Cited by 23 publications

(14 citation statements)

References 35 publications

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“…In strawberry (Fragaria x ananassa), knockdown of RG-lyase expression is correlated with reduced dissolution of the middle lamella during fruit ripening (Molina-Hidalgo et al, 2013). Over-expression of a Solanum lycopersicon homolog resulted in increased tomato fruit firmness (Ochoa-Jim enez et al, 2018). In solution, neutral sugar side chains bind water and increase solubility (Belton, 1997) and reduce intrinsic viscosity, but contribute to polymer entanglement, resulting in higher viscosity at higher RG-I concentrations (Hwang et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Rhamnogalacturonan‐I is a determinant of cell–cell adhesion in poplar wood

Yang

Benatti

Karve

et al. 2019

Plant Biotechnology Journal

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Summary The molecular basis of cell–cell adhesion in woody tissues is not known. Xylem cells in wood particles of hybrid poplar (Populus tremula × P. alba cv. INRA 717‐1B4) were separated by oxidation of lignin with acidic sodium chlorite when combined with extraction of xylan and rhamnogalacturonan‐I (RG‐I) using either dilute alkali or a combination of xylanase and RG‐lyase. Acidic chlorite followed by dilute alkali treatment enables cell–cell separation by removing material from the compound middle lamellae between the primary walls. Although lignin is known to contribute to adhesion between wood cells, we found that removing lignin is a necessary but not sufficient condition to effect complete cell–cell separation in poplar lines with various ratios of syringyl:guaiacyl lignin. Transgenic poplar lines expressing an Arabidopsis thaliana gene encoding an RG‐lyase (AtRGIL6) showed enhanced cell–cell separation, increased accessibility of cellulose and xylan to hydrolytic enzyme activities, and increased fragmentation of intact wood particles into small cell clusters and single cells under mechanical stress. Our results indicate a novel function for RG‐I, and also for xylan, as determinants of cell–cell adhesion in poplar wood cell walls. Genetic control of RG‐I content provides a new strategy to increase catalyst accessibility and saccharification yields from woody biomass for biofuels and industrial chemicals.

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Section: Discussionmentioning

confidence: 99%

Rhamnogalacturonan‐I is a determinant of cell–cell adhesion in poplar wood

Yang

Benatti

Karve

et al. 2019

Plant Biotechnology Journal

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“…The upregulation of rhamnogalacturonate lyase genes, enzymes responsible for hydrolyzing RG-I portions of pectin through the β-elimination mechanism, was particularly of interest. Despite being the first time, these transcripts were found in papaya pulp, they were previously identified in tomatoes and potatoes [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

Systems Biology Applied to the Study of Papaya Fruit Ripening: The Influence of Ethylene on Pulp Softening

Soares

Prado

Andrade

et al. 2021

Cells

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Papaya is a fleshy fruit that undergoes fast ethylene-induced modifications. The fruit becomes edible, but the fast pulp softening is the main factor that limits the post-harvest period. Papaya fast pulp softening occurs due to cell wall disassembling coordinated by ethylene triggering that massively expresses pectinases. In this work, RNA-seq analysis of ethylene-treated and non-treated papayas enabled a wide transcriptome overview that indicated the role of ethylene during ripening at the gene expression level. Several families of transcription factors (AP2/ERF, NAC, and MADS-box) were differentially expressed. ACO, ACS, and SAM-Mtase genes were upregulated, indicating a high rate of ethylene biosynthesis after ethylene treatment. The correlation among gene expression and physiological data demonstrated ethylene treatment can indeed simulate ripening, and regulation of changes in fruit color, aroma, and flavor could be attributed to the coordinated expression of several related genes. Especially about pulp firmness, the identification of 157 expressed genes related to cell wall metabolism demonstrated that pulp softening is accomplished by a coordinated action of several different cell wall-related enzymes. The mechanism is different from other commercially important fruits, such as strawberry, tomato, kiwifruit, and apple. The observed behavior of this new transcriptomic data confirms ethylene triggering is the main event that elicits fast pulp softening in papayas.

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“…RGL genes regulate growth and cohesion between different pectin polymers during plant development and softening during fruit ripening. For instance, the RGL gene participates in pollen tube germination, fruit firmness, and the fruit senescence phenomena that impact postharvest shelf life of tomato ( Ochoa-Jiménez et al, 2018b ). At present, RGLs have been classified into four polysaccharide-lyase (PL) families (PL4, PL9, PL11, and PL26) in the CAZy carbohydrate-active enzyme database 1 .…”

Section: Introductionmentioning

confidence: 99%

Biochemical Characterization of Two Rhamnogalacturonan Lyases From Bacteroides ovatus ATCC 8483 With Preference for RG-I Substrates

Wang

et al. 2022

Front. Microbiol.

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Rhamnogalacturonan lyase (RGL) cleaves backbone α-1,4 glycosidic bonds between L-rhamnose and D-galacturonic acid residues in type I rhamnogalacturonan (RG-I) by β-elimination to generate RG oligosaccharides with various degrees of polymerization. Here, we cloned, expressed, purified and biochemically characterized two RGLs (Bo3128 and Bo4416) in the PL11 family from Bacteroides ovatus ATCC 8483. Bo3128 and Bo4416 displayed maximal activity at pH 9.5 and pH 6.5, respectively. Whereas the activity of Bo3128 could be increased 1.5 fold in the presence of 5 mM Ca2+, Bo4416 required divalent metal ions to show any enzymatic activity. Both of RGLs showed a substrate preference for RG-I compared to other pectin domains. Bo4416 and Bo3128 primarily yielded unsaturated RG oligosaccharides, with Bo3128 also producing them with short side chains, with yields of 32.4 and 62.4%, respectively. Characterization of both RGLs contribute to the preparation of rhamnogalacturonan oligosaccharides, as well as for the analysis of the fine structure of RG-I pectins.

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Functional analysis of tomato rhamnogalacturonan lyase gene Solyc11g011300 during fruit development and ripening

Cited by 23 publications

References 35 publications

Rhamnogalacturonan‐I is a determinant of cell–cell adhesion in poplar wood

Rhamnogalacturonan‐I is a determinant of cell–cell adhesion in poplar wood

Systems Biology Applied to the Study of Papaya Fruit Ripening: The Influence of Ethylene on Pulp Softening

Biochemical Characterization of Two Rhamnogalacturonan Lyases From Bacteroides ovatus ATCC 8483 With Preference for RG-I Substrates

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