Functional and phenotypic distinction of the first two trophoblast subdivisions and identification of the border between them during early postimplantation: A prerequisite for understanding early patterning during placentogenesis

Nikolaou, Stavros; Hadjikypri, Xenia; Ioannou, Giasemia; Elia, Artemis; Georgiades, Pantelis

doi:10.1016/j.bbrc.2017.12.167

Cited by 4 publications

(2 citation statements)

References 30 publications

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“…The placenta is the site of gas and nutrient exchange and elimination of metabolites between the mother and fetus. As a part of the main structure of placenta, the invasion of trophoblast cells into endometrium is an important physiological process of placenta formation [16,17]. Studies have shown that extravillous trophoblast cells invade the uterine decidua and uterine myometrial spiral artery, and gradually replace the endothelial cell layer and some smooth muscle tissue in the maternal spiral artery, resulting in vascular thickening and providing su cient blood supply for the uterus [18,19].…”

Section: Discussionmentioning

confidence: 99%

Endoplasmic reticulum protein 29 inhibits epithelial-mesenchymal-transformation in placental trophoblast cells and promotes the development of intrahepatic cholestasis of pregnancy in pregnant rats

Wang

Dong

Wang

et al. 2022

Preprint

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Background Intrahepatic cholestasis of pregnancy (ICP) is a common pregnancy specific liver disease, which has little impact on the mother and mainly causes adverse fetal outcomes. Endoplasmic reticulum protein 29 (ERp29) plays a key role in protein folding, transport and secretion, and participates in biological events such as apoptosis, oxidative stress, lipid metabolism, immune regulation and so on. Epithelial mesenchymal transformation (EMT) is the process of epithelial cells losing polarity and adhesion, and transforming into mesenchymal like phenotype through migration. Methods Subcutaneous injection of ethinyl estradiol (E2) was used to induce ICP in pregnant rats, and serum TBA, ALT and AST were detected to test whether the animal model was successfully constructed. ERp29 and EMT related molecules were detected by immunohistochemistry and western blotting. Taurocholic acid (TCA) was used to simulate the ICP environment, and TGF-β1 was added to induce the EMT process. CCK8 and TUNEL were used to detect the level of cell apoptosis. The scratch, migration, and invasion test were used to detect the EMT process of cells. ERp29 overexpression/knockdown vector were constructed and transfected to verify the role of ERp29 in the EMT process. Results The ICP animal model was successfully constructed. ERp29 in the placenta tissue of the ICP pregnant rats increased significantly, and the level of apoptosis increased. Compared with the normal, the placental tissues of the ICP group had high expression of E-cadherin and low expression of N-cadherin, snail1, vimentin. After HTR-8/Svneo cells were induced by TCA, EMT was inhibited, while the ERp29 increased. Knockdown of ERp29 reduced the inhibition of cell EMT. After the ERp29 knockdown vector was transfected into pregnant rats by adenovirus, the degree of inhibiting EMT in the placenta tissue of pregnant rats was inhibited, and the ICP progress was alleviated. Conclusion The high level of ERp29 in placental trophoblast cells inhibits the EMT process and aggravates the occurrence and development of ICP.

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Section: Discussionmentioning

confidence: 99%

Endoplasmic reticulum protein 29 inhibits epithelial-mesenchymal-transformation in placental trophoblast cells and promotes the development of intrahepatic cholestasis of pregnancy in pregnant rats

Wang

Dong

Wang

et al. 2022

Preprint

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“…The placenta is the location where gas and nutrient exchange occurs and where metabolic products are eliminated between the mother and the fetus. As part of the main placental structure, invasion of trophoblast cells into the endometrium is an important physiological process in the formation of the placenta ( 35 , 36 ). Studies have shown that extravillous trophoblast cells invade into the uterine decidua and myometrial spiral artery, where they gradually replace the endothelial cell layer and part of the smooth muscle tissue in the maternal spiral arteries, resulting in thickened blood vessels to provide sufficient blood supply to the fetus ( 37 , 38 ).…”

Section: Discussionmentioning

confidence: 99%

CLDN3 expression and function in pregnancy‑induced hypertension

Zhao

2020

Exp Ther Med

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This aim of the present study was to investigate the expression and function of claudin 3 (CLDN3) in pregnancy-induced hypertension. The mRNA expression levels of CLDN3 in the placental tissue and peripheral blood of patients with pregnancy-induced hypertension were measured using reverse transcription-quantitative PCR. Human trophoblast HTR8/SVneo cells overexpressing CLDN3 were generated using a lentiviral vector. Cell Counting kit-8 (CCK-8) assay, flow cytometry, Transwell chamber assays, confocal laser scanning microscopy and western blot analysis were performed to detect cell proliferation, invasion, migration and apoptosis, in addition to matrix metalloproteinase (MMP) expression and ERK1/2 phosphorylation. The mRNA expression levels of CLDN3 were significantly reduced in the placental tissues and peripheral blood samples of patients with pregnancy-induced hypertension compared with healthy pregnant controls. CLDN3 overexpression significantly increased HTR8/SVneo cell proliferation, invasion and migration whilst reducing apoptosis. HTR8/SVneo cells overexpressing CLDN3 also exhibited increased myofiber levels, increased MMP-2 and MMP-9 expression and increased ERK1/2 signaling activity. CLDN3 downregulation may be associated with the pathogenesis of pregnancy-induced hypertension. In conclusion, CLDN3 promotes the proliferative and invasive capabilities of human trophoblast cells, with the underlying mechanisms possibly involving upregulation of MMP expression via the ERK1/2 signaling pathway.

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New findings on the orientation of the mouse anterior-posterior (A-P) axis before and during the initiation of gastrulation using a more refined embryo staging

Hadjikypri,

Theofanous,

Christodoulidi

et al. 2024

Biochemistry and Biophysics Reports

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Functional and phenotypic distinction of the first two trophoblast subdivisions and identification of the border between them during early postimplantation: A prerequisite for understanding early patterning during placentogenesis

Cited by 4 publications

References 30 publications

Endoplasmic reticulum protein 29 inhibits epithelial-mesenchymal-transformation in placental trophoblast cells and promotes the development of intrahepatic cholestasis of pregnancy in pregnant rats

Endoplasmic reticulum protein 29 inhibits epithelial-mesenchymal-transformation in placental trophoblast cells and promotes the development of intrahepatic cholestasis of pregnancy in pregnant rats

CLDN3 expression and function in pregnancy‑induced hypertension

New findings on the orientation of the mouse anterior-posterior (A-P) axis before and during the initiation of gastrulation using a more refined embryo staging

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