Functional and proteomic analysis of submandibular saliva in rats exposed to chronic stress by immobilization or constant light

Alterman, Alan; Mathison, Ronald; Coronel, Carlos E.; Stroppa, María Mercedes; Finkelberg, A.; Gallará, Raquel Vivian

doi:10.1016/j.archoralbio.2011.12.008

Cited by 15 publications

(14 citation statements)

References 45 publications

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“…Then, the salivette was centrifuged at 1000 × g for 2 min in order to recover the whole saliva (WS). The lower salivary flux of the rat and rabbit, comparatively to the other species, was taken in consideration by stimulating the salivation of those mammals . Thus, saliva from rat was collected under anesthesia induced by intraperitoneal injection of 90 mg/kg ketamine (Imalgene 1000, Merial) and 10 mg/kg xilazine (Rompum 2%, Bayer HearthCare), followed by the stimulation with sialagogue pilocarpine in sterile PBS (4 μmol/kg), through intraperitoneal injection.…”

Section: Methodsmentioning

confidence: 99%

Cross-species comparison of mammalian saliva using an LC-MALDI based proteomic approach

Sousa-Pereira

Cova

Abrantes³

et al. 2015

Proteomics

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Despite the importance of saliva in the regulation of oral cavity homeostasis, few studies have been conducted to quantitatively compare the saliva of different mammal species. Aiming to define a proteome signature of mammals' saliva, an in-depth SDS-PAGE-LC coupled to MS/MS (GeLC-MS/MS) approach was used to characterize the saliva from primates (human), carnivores (dog), glires (rat and rabbit), and ungulates (sheep, cattle, horse). Despite the high variability in the number of distinct proteins identified per species, most protein families were shared by the mammals studied with the exception of cattle and horse. Alpha-amylase is an example that seems to reflect the natural selection related to digestion efficacy and food recognition. Casein protein family was identified in all species but human, suggesting an alternative to statherin in the protection of hard tissues. Overall, data suggest that different proteins might assure a similar role in the regulation of oral cavity homeostasis, potentially explaining the specific mammals' salivary proteome signature. Moreover, some protein families were identified for the first time in the saliva of some species, the presence of proline-rich proteins in rabbit's saliva being a good example.

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Section: Methodsmentioning

confidence: 99%

Cross-species comparison of mammalian saliva using an LC-MALDI based proteomic approach

Sousa-Pereira

Cova

Abrantes³

et al. 2015

Proteomics

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“…The total food intake of rats in LL was mostly unaffected [56,58,76,77], but the distribution changed, causing the gradual loss of rhythmicity corresponding with changes in locomotor activity and neuronal activity in SCN [78]. The total food and water intake was reduced in Long Evans rats, probably due to strain differences or short-term exposure [55].…”

Section: Food Intake and Body Mass Under Constant Lightmentioning

confidence: 99%

“…Despite this, the food efficiency was higher, therefore, rats gained more weight per gram of consumed food, although no change in body weight was noticed. Even though the distribution of food intake was altered, the body weight was not affected in rats [54][55][56]58,76,77], but still, the higher visceral adiposity was found [55], as indicated in Table 1. Only one study reported increased body weight and fat mass in Wistar rats after LL exposure [57].…”

Section: Food Intake and Body Mass Under Constant Lightmentioning

confidence: 99%

Differential Effects of Constant Light and Dim Light at Night on the Circadian Control of Metabolism and Behavior

Rumanova

Okuliarova

Zeman

2020

IJMS

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The disruption of circadian rhythms by environmental conditions can induce alterations in body homeostasis, from behavior to metabolism. The light:dark cycle is the most reliable environmental agent, which entrains circadian rhythms, although its credibility has decreased because of the extensive use of artificial light at night. Light pollution can compromise performance and health, but underlying mechanisms are not fully understood. The present review assesses the consequences induced by constant light (LL) in comparison with dim light at night (dLAN) on the circadian control of metabolism and behavior in rodents, since such an approach can identify the key mechanisms of chronodisruption. Data suggest that the effects of LL are more pronounced compared to dLAN and are directly related to the light level and duration of exposure. Dim LAN reduces nocturnal melatonin levels, similarly to LL, but the consequences on the rhythms of corticosterone and behavioral traits are not uniform and an improved quantification of the disrupted rhythms is needed. Metabolism is under strong circadian control and its disruption can lead to various pathologies. Moreover, metabolism is not only an output, but some metabolites and peripheral signal molecules can feedback on the circadian clockwork and either stabilize or amplify its desynchronization.

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“…Various mass spectrometry methods are currently available and have been widely used to study the salivary proteins, and smaller, endogenous peptides. However, despite extensive investigation in humans [ 2 , 5 , 8 , 10 – 26 ], much less work has been done in other species [ 3 , 27 – 34 ]. Recently, the protein components of dog saliva were examined using SDS-PAGE-LC coupled to tandem mass spectrometry (MS/MS) [ 3 ], but only one dog sample was analyzed.…”

Section: Introductionmentioning

confidence: 99%

Salivary proteomics of healthy dogs: An in depth catalog

et al. 2018

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ObjectiveTo provide an in-depth catalog of the salivary proteome and endogenous peptidome of healthy dogs, evaluate proteins and peptides with antimicrobial properties, and compare the most common salivary proteins and peptides between different breed phylogeny groups.Methods36 healthy dogs without evidence of periodontal disease representing four breed phylogeny groups, based upon single nucleotide polymorphism haplotypes (ancient, herding/sighthound, and two miscellaneous groups). Saliva collected from dogs was pooled by phylogeny group and analyzed using nanoscale liquid chromatography-tandem mass spectrometry. Resulting tandem mass spectra were compared to databases for identification of endogenous peptides and inferred proteins.Results2,491 proteins and endogenous peptides were found in the saliva of healthy dogs with no periodontal disease. All dog phylogeny groups’ saliva was rich in proteins and peptides with antimicrobial functions. The ancient breeds group was distinct in that it contained unique proteins and was missing many proteins and peptides present in the other groups.Conclusions and clinical relevanceUsing a sophisticated nanoscale liquid chromatography-tandem mass spectrometry, we were able to identify 10-fold more salivary proteins than previously reported in dogs. Seven of the top 10 most abundant proteins or peptides serve immune functions and many more with various antimicrobial mechanisms were found. This is the most comprehensive analysis of healthy canine saliva to date, and will provide the groundwork for future studies analyzing salivary proteins and endogenous peptides in disease states.

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Functional and proteomic analysis of submandibular saliva in rats exposed to chronic stress by immobilization or constant light

Cited by 15 publications

References 45 publications

Cross-species comparison of mammalian saliva using an LC-MALDI based proteomic approach

Cross-species comparison of mammalian saliva using an LC-MALDI based proteomic approach

Differential Effects of Constant Light and Dim Light at Night on the Circadian Control of Metabolism and Behavior

Salivary proteomics of healthy dogs: An in depth catalog

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