Functional characterization and transcriptome analysis reveal multiple roles for prc in the pathogenicity of the black rot pathogen Xanthomonas campestris pv. campestris

Liao, Chao-Tsai; Liu, Yu-Fan; Chiang, Ying-Chuan; Lo, Hsueh-Hsia; Du, Shin-Chiao; Hsu, Pei-Chi; Hsiao, Yi‐Min

doi:10.1016/j.resmic.2016.01.002

Cited by 21 publications

(27 citation statements)

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“…The structure presented in this work may facilitate design of specific inhibitors targeting the proteolytic activity of Prc. Recent studies have suggested that the activity of Prc may contribute to the pathogenicity and virulence of several Gram-negative pathogens 17 , 18 ; suppressing Prc activity may prevent evasion of E. coli from complement-mediated serum killing 18 . Inhibiting the activity of Prc or its NlpI-interaction results in excessive cross-link hydrolysis and may affect the viability of Gram-negative bacteria.…”

Section: Discussionmentioning

confidence: 99%

Structural basis of adaptor-mediated protein degradation by the tail-specific PDZ-protease Prc

Som

et al. 2017

Nat Commun

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Peptidoglycan (PG) is a highly cross-linked, protective mesh-like sacculus that surrounds the bacterial cytoplasmic membrane. Expansion of PG is tightly coupled to growth of a bacterial cell and requires hydrolases to cleave the cross-links for insertion of nascent PG material. In Escherichia coli, a proteolytic system comprising the periplasmic PDZ-protease Prc and the lipoprotein adaptor NlpI contributes to PG enlargement by regulating cellular levels of MepS, a cross-link-specific hydrolase. Here, we demonstrate how NlpI binds Prc to facilitate the degradation of its substrate MepS by structural and mutational analyses. An NlpI homodimer binds two molecules of Prc and forms three-sided MepS-docking cradles using its tetratricopeptide repeats. Prc forms a monomeric bowl-shaped structure with a lid-like PDZ domain connected by a substrate-sensing hinge that recognizes the bound C terminus of the substrate. In summary, our study reveals mechanistic details of protein degradation by the PDZ-protease Prc bound to its cognate adaptor protein.

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Section: Discussionmentioning

confidence: 99%

Structural basis of adaptor-mediated protein degradation by the tail-specific PDZ-protease Prc

Som

et al. 2017

Nat Commun

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“…In our previous work, a mutant library of Xcc17 was constructed using EZ‐Tn 5 TM <R6Kγ ori /KAN‐2> Tnp Transposome Kit . Approximately, 1000 EZ‐Tn 5 mutants were subjected to attachment assay for screening for biofilm‐altered mutants with aims to identify genes involved in biofilm formation.…”

Section: Resultsmentioning

confidence: 99%

“…Since several factors playing a role in bacterial attachment are involved in pathogenicity in Xcc [12][13][14]17,18], we aimed to determine whether the wxcX gene is associated with the virulence of Xcc. The wxcX mutant revealed reduced virulence compared with the wild type (Fig.…”

Section: Wxcx Is Required For the Full Virulence Of Xccmentioning

confidence: 99%

“…The role of biofilm formation in the virulence of plant pathogenic bacteria has been reported for a range of bacterial infections in plants, such as Erwinia amylovora , the causal agent of fire blight, as well as Xylella fastidiosa , the causal agent of oak leaf scorch . In Xcc, several factors having a role in biofilm formation or bacterial adhesion are involved in virulence, including exopolysaccharide , extracellular mannanase ManA , GGDEF domain proteins XCC2731 and XC_0249 , guanylate cyclase XC_0250 , isocitrate dehydrogenase encoded by icd2 , major outer‐membrane protein MopB , RavA/RavR two‐component system , RNA‐binding protein RsmA , tail‐specific protease encoded by prc , and UTP‐glucose‐1‐phosphate uridylyltransferase encoded by galU .…”

Section: Introductionmentioning

confidence: 99%

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WxcX is involved in bacterial attachment and virulence in Xanthomonas campestris pv. campestris

et al. 2018

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Xanthomonas campestris pv. campestris (Xcc) is the causative agent of black rot in crucifers. Here, one EZ-Tn5 transposon mutant of Xcc, altered in bacterial attachment, was isolated. Further analysis revealed that the transposon was inserted in the wxcX gene (encodes a hypothetical protein) of the transposon mutant. Sequence analysis revealed that WxcX is highly conserved in Xanthomonas, but none has been characterized. In this study, it was indicated that mutation of wxcX resulted in enhanced bacterial attachment, reduced virulence on the host cabbage, and increased sensitivity to sodium dodecyl sulfate. The affected phenotypes of the wxcX mutant could be complemented to wild-type levels by the intact wxcX gene. Site-directed mutagenesis revealed that E408 and E411 are critical amino acid residues for WxcX function in bacterial attachment. Taken together, our results demonstrate the roles of wxcX in attachment, virulence, and tolerance to sodium dodecyl sulfate in Xanthomonas for the first time.

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“…A goma xantana (GX) é um biopolímero natural, produzido por bactérias fitopatogênica do gênero Xanthomonas, que apresenta células em forma de bastonetes, são Gram-negativas e ocorrem predominantemente isoladas (Wibberg et al, 2015;Brandão et al, 2010). A espécie mais utilizada é a Xanthomonas campestris, agente responsável por causar a podridão negra em crucíferas, como repolho, couve-flor, brócolis, couves de bruxelas e couve (Liao et al, 2016), pela produção em larga escala do exopolissicarídeo tendo aplicações nas indústrias alimentícia, cosmética, farmacêutica e petrolífera (Badwaik et., 2016;Silva & Schmidt, 2015).…”

Section: Introductionunclassified

Technological Prospection of Patents of Xanthan Gum in the Food Industry Context

SILVA¹,

Druzian²

2020

RGEINTEC

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Foi realizado o estudo da prospecção tecnológica da aplicação de goma xantana não combinada com outras gomas microbianas em alimentos ou gêneros alimentícios, usando como fontes de informações o banco de dados do Espacenet (EP). A metodologia de pesquisa consistiu na utilização das palavras-chaves "Xanthan Gum*", "Xanthomonas campestris*" e "Exopolysaccharide*" resultando no estudo dos códigos "A23L" e "A23L29/27". Foram analisados a evolução do número de depósitos de patentes, a distribuição de depósitos por país de origem das tecnologias, o número de patentes por código CPC (Classificação de Patentes Cooperativa) e a distribuição da aplicabilidade da xantana nas inovações. São diversas as aplicações de goma xantana na área alimentícia indicando a importância do uso do biopolímero na preparação, modificação de qualidades nutritivas e conservação dos alimentos ou gêneros alimentícios.

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Functional characterization and transcriptome analysis reveal multiple roles for prc in the pathogenicity of the black rot pathogen Xanthomonas campestris pv. campestris

Cited by 21 publications

References 77 publications

Structural basis of adaptor-mediated protein degradation by the tail-specific PDZ-protease Prc

Structural basis of adaptor-mediated protein degradation by the tail-specific PDZ-protease Prc

WxcX is involved in bacterial attachment and virulence in Xanthomonas campestris pv. campestris

Technological Prospection of Patents of Xanthan Gum in the Food Industry Context

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