Functional Characterization of Date Palm Aquaporin Gene PdPIP1;2 Confers Drought and Salinity Tolerance to Yeast and Arabidopsis

Patankar, Himanshu V.; Al-Harrasi, Ibtisam; Al-Yahyai, Rashid; Yaish, Mahmoud W.

doi:10.3390/genes10050390

Cited by 36 publications

(30 citation statements)

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“…The AQP family in these three species has been systematically characterized, providing valuable information for further functional analysis to infer the roles of AQPs in adaptation to diverse environmental conditions [ 45 , 47 , 48 ]. Psammophytes jojoba ( Simmondsia chinensis ), date palm ( Phoenix dactylifera ), and jujube ( Ziziphus jujuba ) can all tolerate drought, salinity, and nutrient stress, and their AQP genes have been intensively studied to better understand their potential for genetic engineering to improve plant stress tolerance [ 39 , 49 , 50 ]. The halophytes Atriplex canescens , Sesuvium portulacastrum , and Thellungiella salsuginea all exhibit strong salt tolerance, and their PIP genes have been found to function in the response to salt stress and could be used in genetic engineering to improve plant growth under abiotic stress [ 33 , 35 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…As a unicellular eukaryote with a rapid growth rate, yeast has become a convenient protein expression system for protein functional identification. We found that inducing the expression of CrPIP2;3 in yeast ( Figure S4A ) resulted in obvious sensitivity to high salt and high osmotic stress ( Figure 4 ), which is, to some extent, opposite to that observed in other plant PIP members, such as barley PIP2;5 ( HvPIP2;5 ), foxtail millet SiPIP3;1 and SiSIP1;1 , and date palm PdPIP1;2 , which improved salt and osmotic stress tolerance in yeast [ 49 , 52 , 53 ]. Similarly, mammalian AQP1 and AQP5 caused moderate growth inhibition under salt and hyperosmotic stress when expressed in yeast [ 54 ], and many AQPs have been confirmed as membrane transporters of H 2 O 2 in the plant response to stress [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

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Ectopic Expression of CrPIP2;3, a Plasma Membrane Intrinsic Protein Gene from the Halophyte Canavalia rosea, Enhances Drought and Salt-Alkali Stress Tolerance in Arabidopsis

Zheng

Lin

et al. 2021

IJMS

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Aquaporins are channel proteins that facilitate the transmembrane transport of water and other small neutral molecules, thereby playing vital roles in maintaining water and nutrition homeostasis in the life activities of all organisms. Canavalia rosea, a seashore and mangrove-accompanied halophyte with strong adaptability to adversity in tropical and subtropical regions, is a good model for studying the molecular mechanisms underlying extreme saline-alkaline and drought stress tolerance in leguminous plants. In this study, a PIP2 gene (CrPIP2;3) was cloned from C. rosea, and its expression patterns and physiological roles in yeast and Arabidopsis thaliana heterologous expression systems under high salt-alkali and high osmotic stress conditions were examined. The expression of CrPIP2;3 at the transcriptional level in C. rosea was affected by high salinity and alkali, high osmotic stress, and abscisic acid treatment. In yeast, the expression of CrPIP2;3 enhanced salt/osmotic and oxidative sensitivity under high salt/osmotic and H2O2 stress. The overexpression of CrPIP2;3 in A. thaliana could enhance the survival and recovery of transgenic plants under drought stress, and the seed germination and seedling growth of the CrPIP2;3 OX (over-expression) lines showed slightly stronger tolerance to high salt/alkali than the wild-type. The transgenic plants also showed a higher response level to high-salinity and dehydration than the wild-type, mostly based on the up-regulated expression of salt/dehydration marker genes in A. thaliana plants. The reactive oxygen species (ROS) staining results indicated that the transgenic lines did not possess stronger ROS scavenging ability and stress tolerance than the wild-type under multiple stresses. The results confirmed that CrPIP2;3 is involved in the response of C. rosea to salt and drought, and primarily acts by mediating water homeostasis rather than by acting as an ROS transporter, thereby influencing physiological processes under various abiotic stresses in plants.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Ectopic Expression of CrPIP2;3, a Plasma Membrane Intrinsic Protein Gene from the Halophyte Canavalia rosea, Enhances Drought and Salt-Alkali Stress Tolerance in Arabidopsis

Zheng

Lin

et al. 2021

IJMS

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“…Studies on the response of date palm to abiotic stresses are limited and the explicit mechanisms of stress tolerance are yet to be identified. The transcriptome, methylome and miRNAome of salinity-stressed date palm offer some insights into the complex abiotic stress tolerance mechanisms [12,13,14,15,16,17]. Specifically, the global gene expression profiles of salt-stressed date palm have identified a differentially expressed genetic makeup [12].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of a Metallothionein 2A Gene from Date Palm Confers Abiotic Stress Tolerance to Yeast and Arabidopsis thaliana

Patankar

Al-Harrasi

Kharusi

et al. 2019

IJMS

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Although the date palm tree is an extremophile with tolerance to drought and certain levels of salinity, the damage caused by extreme salt concentrations in the soil, has created a need to explore stress-responsive traits and decode their mechanisms. Metallothioneins (MTs) are low-molecular-weight cysteine-rich proteins that are known to play a role in decreasing oxidative damage during abiotic stress conditions. Our previous study identified date palm metallothionein 2A (PdMT2A) as a salt-responsive gene, which has been functionally characterized in yeast and Arabidopsis in this study. The recombinant PdMT2A protein produced in Escherichia coli showed high reactivity against the substrate 5′-dithiobis-2-nitrobenzoic acid (DTNB), implying that the protein has the property of scavenging reactive oxygen species (ROS). Heterologous overexpression of PdMT2A in yeast (Saccharomyces cerevisiae) conferred tolerance to drought, salinity and oxidative stresses. The PdMT2A gene was also overexpressed in Arabidopsis, to assess its stress protective function in planta. Compared to the wild-type control, the transgenic plants accumulated less Na+ and maintained a high K+/Na+ ratio, which could be attributed to the regulatory role of the transgene on transporters such as HKT, as demonstrated by qPCR assay. In addition, transgenic lines exhibited higher chlorophyll content, higher superoxide dismutase (SOD) activity and improved scavenging ability for reactive oxygen species (ROS), coupled with a better survival rate during salt stress conditions. Similarly, the transgenic plants also displayed better drought and oxidative stress tolerance. Collectively, both in vitro and in planta studies revealed a role for PdMT2A in salt, drought, and oxidative stress tolerance.

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“…In a related study, Patankar et al (2019b) expressed date palm aquaporin PdPIP1;2 in yeast and reported improved tolerance to salinity and oxidative stresses. On the other hand, overexpression of the same gene in Arabidopsis showed symptoms of improved tolerance including enhanced biomass, chlorophyll content, root length under salt and drought conditions and high K + /Na + compared to wild type.…”

Section: Salinity Salinity Tolerance In Date Palmsmentioning

confidence: 99%

Prospects for the Study and Improvement of Abiotic Stress Tolerance in Date Palms in the Post-genomics Era

et al. 2020

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Date palm (Phoenix dactylifera L.) is a socioeconomically important crop in the Middle East and North Africa and a major contributor to food security in arid regions of the world. P. dactylifera is both drought and salt tolerant, but recent water shortages and increases in groundwater and soil salinity have threatened the continued productivity of the crop. Recent studies of date palm have begun to elucidate the physiological mechanisms of abiotic stress tolerance and the genes and biochemical pathways that control the response to these stresses. Here we review recent studies on tolerance of date palm to salinity and drought stress, the role of the soil and root microbiomes in abiotic stress tolerance, and highlight recent findings of omic-type studies. We present a perspective on future research of abiotic stress in date palm that includes improving existing genome resources, application of genetic mapping to determine the genetic basis of variation in tolerances among cultivars, and adoption of gene-editing technologies to the study of abiotic stress in date palms. Development of necessary resources and application of the proposed methods will provide a foundation for future breeders and genetic engineers aiming to develop more stress-tolerant cultivars of date palm.

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Functional Characterization of Date Palm Aquaporin Gene PdPIP1;2 Confers Drought and Salinity Tolerance to Yeast and Arabidopsis

Cited by 36 publications

References 53 publications

Ectopic Expression of CrPIP2;3, a Plasma Membrane Intrinsic Protein Gene from the Halophyte Canavalia rosea, Enhances Drought and Salt-Alkali Stress Tolerance in Arabidopsis

Ectopic Expression of CrPIP2;3, a Plasma Membrane Intrinsic Protein Gene from the Halophyte Canavalia rosea, Enhances Drought and Salt-Alkali Stress Tolerance in Arabidopsis

Overexpression of a Metallothionein 2A Gene from Date Palm Confers Abiotic Stress Tolerance to Yeast and Arabidopsis thaliana

Prospects for the Study and Improvement of Abiotic Stress Tolerance in Date Palms in the Post-genomics Era

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