Functional characterization of highly adherent CD34+ keratinocytes isolated from human skin

Gutiérrez-Rivera, Araika; Pavón-Rodríguez, Andrés; Jiménez‐Acosta, F.; Poblet, Enrique; Braun, Kristin M.; Cormenzana, Pedro; Ciria, Juan Pablo; Larretxea, Rafael; Cardenas, Jose M.; Izeta, Ander

doi:10.1111/j.1600-0625.2010.01075.x

Cited by 20 publications

(18 citation statements)

References 36 publications

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“…In addition, the colony‐forming ability of CD34‐positive cells was significantly higher than that of CD34‐negative cells (Fig. 2c) in contrast to epidermal keratinocytes (30). In addition, CD34‐positive cells isolated from human scalp tissues showed a greater ability to differentiate into mesenchymal lineages (Figs 2d and S3).…”

Section: Resultsmentioning

confidence: 92%

Perivascular localization of dermal stem cells in human scalp

Yamanishi

Fujiwara

Soma

2011

Experimental Dermatology

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In mammalian skin, the existence of stem cells in the dermis is still poorly understood. Previous studies have indicated that mesenchymal stem cells (MSCs) are situated as pericytes in various mammalian tissues. We speculated that the human adult dermis also contains MSC-like cells positive for CD34 at perivascular sites similar to adipose tissue. At first, stromal cells from adult scalp skin tissues showed colony-forming ability and differentiated into mesenchymal lineages (osteogenic, chondrogenic and adipogenic). Three-dimensional analysis of scalp skin with a confocal microscope clearly demonstrated that perivascular cells were positive for not only NG2, but also CD34, immunoreactivity. Perivascular CD34-positive cells were abundant around follicular portions. Furthermore, CD34-positive cell fractions collected with magnetic cell sorting were capable of differentiating into mesenchymal lineages. This study suggests that dermal perivascular sites act as a niche of MSCs in human scalp skin, which are easily accessible and useful in regenerative medicine.

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Section: Resultsmentioning

confidence: 92%

Perivascular localization of dermal stem cells in human scalp

Yamanishi

Fujiwara

Soma

2011

Experimental Dermatology

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“…This indicates that p75 NTR identifies a population of early TA cells. A population of early TA has been already detected in the hair follicle (74) and in the interfollicular epidermis (75) and appears to be critical in the first steps of the differentiation process (76). p75 NTR protein that exerts its activities at the boundary between KSC and TA could function as early trigger of keratinocyte differentiation.…”

Section: P75ntr and Epidermal Homeostasismentioning

confidence: 99%

p75 Neurotrophin Receptor in the Skin: Beyond Its Neurotrophic Function

Pincelli

2017

Front. Med.

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p75 neurotrophin receptor (p75NTR), also known as CD271, is the low-affinity receptor that, together with the tyrosine kinase receptor tropomyosin-receptor kinase (Trk), mediate neurotrophin (NT) functions. Beside their classic role in skin innervation, NT and their receptors constitute a complex cutaneous network associated with a number of autocrine and paracrine activities. In this context, the role of p75NTR is becoming more and more important. This review will focus on the intriguing functions of p75NTR in healthy and diseased skin. First, p75NTR counterbalances the proliferative and survival activities of its cognate receptor Trk by inducing keratinocyte apoptosis. In addition, p75NTR identifies an early transit-amplifying (TA) keratinocyte population and plays a critical role in keratinocyte stem cell transition to its progeny as well as in epidermal differentiation. p75NTR is absent in psoriatic TA cells, thus rendering these cells resistant to apoptosis. On the other hand, p75NTR infection restores NT-induced apoptosis in psoriatic keratinocytes. Taken together, these results provide evidence for a critical role of p75NTR in epidermal homeostasis, while its lack may account for the TA defect in psoriasis. While the issue of p75NTR as a marker of melanoma initiating cells is still to be solved, there is strong evidence that downregulation of this receptor is a precondition to melanoma invasion and metastasis in vitro and in vivo. All in all, this review points to p75NTR as a major actor in both physiologic and pathologic conditions at the skin level.

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“…CD34 (cluster of differentiation 34), a single-pass transmembrane sialomucin protein, marks bulge cells in murine follicles [22], but is absent from human bulge tissue [23,61]. In murine hair follicle bulge cells, the CD34 + cells are label-retaining and readily form clonal colonies [118,119].…”

Section: Stem Cells Of the Hair Folliclementioning

confidence: 99%

Biochemistry of epidermal stem cells

Eckert

Adhikary

Balasubramanian

et al. 2013

Biochimica et Biophysica Acta (BBA) - General Subjects

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Background The epidermis is an important protective barrier that is essential for maintenance of life. Maintaining this barrier requires continuous cell proliferation and differentiation. Moreover, these processes must be balanced to produce a normal epidermis. The stem cells of the epidermis reside in specific locations in the basal epidermis, hair follicle and sebaceous glands and these cells are responsible for replenishment of this tissue. Scope of review A great deal of effort has gone into identifying protein epitopes that mark stem cells, in identifying stem cell niche locations, and in understanding how stem cell populations are related. We discuss these studies as they apply to understanding normal epidermal homeostasis and skin cancer. Major conclusions An assortment of stem cell markers have been identified that permit assignment of stem cells to specific regions of the epidermis, and progress has been made in understanding the role of these cells in normal epidermal homeostasis and in conditions of tissue stress. A key finding is the multiple stem cell populations exist in epidermis that give rise to different structures, and that multiple stem cell types may contribute to repair in damaged epidermis. General significance Understanding epidermal stem cell biology is likely to lead to important therapies for treating skin diseases and cancer, and will also contribute to our understanding of stem cells in other systems. This article is part of a Special Issue entitled Biochemistry of Stem Cells.

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Functional characterization of highly adherent CD34+ keratinocytes isolated from human skin

Cited by 20 publications

References 36 publications

Perivascular localization of dermal stem cells in human scalp

Perivascular localization of dermal stem cells in human scalp

p75 Neurotrophin Receptor in the Skin: Beyond Its Neurotrophic Function

Biochemistry of epidermal stem cells

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