Functional Characterization of Mung Bean Meal Protein-Derived Antioxidant Peptides

Abstract

The aim of this work was to characterize the antioxidant properties of some of the peptides present in bromelain mung bean meal protein hydrolysate (MMPH). The MMPH was subjected to two rounds of bioassay-guided reversed-phase HPLC separation followed by peptide identification in the most potent fractions using tandem mass spectrometry. Twelve antioxidant peptides, namely, HC, CGN, LAN, CTN, LAF, CSGD, MMGW, QFAAD, ERF, EYW, FLQL, and QFAW were identified and assayed for antioxidant properties. CTN, HC, CGN, and CSGD were the most potent (p < 0.05) DPPH radical scavengers with EC50 values of 0.30, 0.29, 0.28, and 0.30 mg/mL, respectively, which are lower than the 0.03 mg/mL obtained for reduced glutathione (GSH). CTN, HC, CGN, and CSGD exhibited the most potent (p < 0.05) scavenging activities against hydroxyl and superoxide radicals with EC50 values that are similar to those of GSH. The cysteine-containing peptides also had stronger ferric reducing antioxidant power and metal chelation activity than peptides devoid of cysteine. In contrast, MMGW, ERF, and EYW had poor radical scavenging and metal chelation activities. We conclude that the availability of the sulfhydryl group may have enhanced antioxidant potency while the presence of bulky groups such phenylalanine and tryptophan had an opposite effect.

Effect of Protease Type and Peptide Size on the In Vitro Antioxidant, Antihypertensive and Anti-Diabetic Activities of Eggplant Leaf Protein Hydrolysates

“…This is consistent with the role of cysteine as a major factor in the antioxidant potency of GSH. The results are similar to previously reported data showing that SCAAs are important for the strong DRSA of peptides [ 54 , 55 ]. The stronger DRSA of CH may be related to the presence of a higher number and wider variety of short-chain peptides (tri- and tetrapeptides) when compared to the longer size peptides and narrower structural features of the other hydrolysates, as shown in Table 2 .…”

Production, Purification, and Potential Health Applications of Edible Seeds’ Bioactive Peptides: A Concise Review

“…EC 50 is the half-maximal effective concentration, which means concentrations at which the substance/sample/drug is expected to produce 50% of the effect. Moreover, the findings also showed that CSGD, CGN, HC, and CTN had the most potent superoxide and hydroxyl radical scavenging activities [73]. The protein hydrolysate from defatted peanut kernel protein was also reported to show antioxidative properties.…”

Transepithelial transport and cellular mechanisms of food-derived antioxidant peptides

“…OH and . O 2 scavenging assays, peptides (CTN, HC, CGN, and CSGD) isolated from mung bean meal protein hydrolysate were shown to have antioxidant activities that were comparable to the effect of reduced glutathione [ 39 ]. Cysteine-containing peptides also show high antioxidant activities [ 31 , 33 , 40 ].…”