Functional characterization of recombinant gonad-inhibiting hormone (GIH) and implication of antibody neutralization on induction of ovarian maturation in marine shrimp

Treerattrakool, Supattra; Boonchoy, Chanikarn; Urtgam, Sittichai; Panyim, Sakol; Udomkit, Apinunt

doi:10.1016/j.aquaculture.2014.03.009

Cited by 47 publications

(32 citation statements)

References 19 publications

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“…Even though the recombinant CHH-B1 peptide has no additional tags that aid its purification, it was successfully purified by RP-HPLC. This purification strategy has been used before to obtain other bioactive recombinant CHH-family peptides expressed in P. pastoris, for example Pem-CHH1-3, Pem-MIH1 and Pem-GIH from Penaeus monodon [39,40,43,51], and Pej-SGP-III from Penaeus japonicus [28]. At the protein level, the consensus appears to be that the eyestalk predominantly expresses CHH isoforms with C-terminal amidation that show significant induction of hyperglycemia, suppression of ecdysteroid synthesis, or both, whereas the extraeyestalk tissues predominantly express CHH-L isoforms that have a free C-terminus and have generally been reported not to have hyperglycemic activity [2,10,29].…”

Section: Discussionmentioning

confidence: 99%

Hyperglycemic activity of the recombinant crustacean hyperglycemic hormone B1 isoform (CHH-B1) of the Pacific white shrimp Litopenaeus vannamei

et al. 2015

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Section: Discussionmentioning

confidence: 99%

Hyperglycemic activity of the recombinant crustacean hyperglycemic hormone B1 isoform (CHH-B1) of the Pacific white shrimp Litopenaeus vannamei

et al. 2015

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“…The expression and purification of the recombinant GIH (rGIH) were carried out as described by Treerattrakool et al. (). Briefly, P. pastoris KM71 carrying the expression cassette for rGIH was cultured in YPD (1% yeast extract, 2% peptone and 2% D‐glucose) containing 100 μg/ml zeocin at 30°C for 2 days.…”

Section: Methodsmentioning

confidence: 99%

In vitro study of a putative role of gonad-inhibiting hormone in oocyte growth stimulation inPenaeus monodon

2017

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Ovarian development in crustacean is controlled by several factors, among which a neuropeptide gonad‐inhibiting hormone (GIH) is known to inhibit vitellogenin (Vg) synthesis in the ovary. It has been postulated that GIH may control Vg synthesis by inhibiting the release of gonad‐stimulating factor (GSF) from brain and thoracic ganglia. To prove this hypothesis, this study was primarily aimed to investigate the influence of GIH on the release of GSF from thoracic ganglia of Penaeus monodon. Our result showed that GIH did not suppress the release of putative GSF from thoracic ganglia by calcium ionophore A23187 as the induction of oocyte growth in the ovary explants that were cocultured with thoracic ganglia in the presence of A23187 was not affected by the addition of recombinant GIH protein. In addition and interestingly, when the ovary explants were incubated with the recombinant GIH alone, the oocyte growth was increased at the rate comparable to that induced by A23187 in the presence of thoracic ganglia. Hence, our in vitro study demonstrated that the stimulation of GSF released from thoracic ganglia is independent of GIH, and that the GIH has a dual function in oocyte growth stimulation and inhibition of Vg synthesis in the early stage of ovarian development. This expands our knowledge on the regulation of ovarian development in shrimp by GIH. Further in vivo studies in this novel aspect of GIH function will be useful for the improvement of shrimp ovarian maturation in the future.

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“…In the lobster Homarus americanus, GIH levels in hemolymph were high during the immature and previtellogenic stages and low during maturation (De Kleijn et al, 1998). Furthermore, the injection of anti-GIH antibodies can produce ovarian maturation and spawning effects similar to those resulting from unilateral eyestalk ablation in Penaeus monodon (Treerattrakool et al, 2014). In Metanephrops japonicus and Litopenaeus vannamei, recombinant GIH administration can significantly reduce Vtg mRNA levels (Ohira et al, 2005;Chen et al, 2014).…”

Section: Introductionmentioning

confidence: 97%

In vivo and in vitro inhibitory action of 17β-estradiol and environmental estrogen 4-nonylphenol on gonad-inhibiting hormone (GIH) expression in the eyestalks of Litopenaeus vannamei

Chen

Deng

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The gonad-inhibiting hormone (GIH) belongs to a neuropeptide family synthesized and released in an X-organ sinus gland complex of crustacean eyestalks. GIH inhibits crustacean ovarian maturation by suppressing vitellogenin (Vtg) synthesis, whereas estrogen is responsible for the stimulation of vitellogenesis (not established). In this study, the effects of 17β-estradiol (E 2 , 10 -6 M), estrogen receptor antagonist tamoxifen (TAM, 10 -6 , 10 -7 , and 10 -8 M), and the environmental estrogen nonylphenol (NP, 1 μg/L and 100 μg/L) on LvGIH expression in the eyestalks of shrimp were determined by quantitative real-time PCR. Results showed that LvGIH expression decreased significantly during the L. vannamei ovarian maturation cycle. E 2 and NP significantly reduced LvGIH transcripts in 14057 GIH expression with estrogen treatment in L. vannamei ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 14056-14065 (2015) vivo, but TAM neutralized the inhibitory action of E 2 in a dose-dependent manner (P < 0.05). In addition, the LvGIH expression levels decreased significantly in a time-dependent manner (P < 0.05) when ovary fragments were cultured in vitro with E 2 . The results of this study suggested that estrogen regulates GIH expression in L. vannamei eyestalks. E 2 promoted ovarian development not only by directly upregulating vitellogenesis in the hepatopancreas, but it was also capable of downregulating LvGIH expression, which indirectly resulted in the stimulation of L. vannamei vitellogenesis.

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Functional characterization of recombinant gonad-inhibiting hormone (GIH) and implication of antibody neutralization on induction of ovarian maturation in marine shrimp

Cited by 47 publications

References 19 publications

Hyperglycemic activity of the recombinant crustacean hyperglycemic hormone B1 isoform (CHH-B1) of the Pacific white shrimp Litopenaeus vannamei

Hyperglycemic activity of the recombinant crustacean hyperglycemic hormone B1 isoform (CHH-B1) of the Pacific white shrimp Litopenaeus vannamei

In vitro study of a putative role of gonad-inhibiting hormone in oocyte growth stimulation inPenaeus monodon

In vivo and in vitro inhibitory action of 17β-estradiol and environmental estrogen 4-nonylphenol on gonad-inhibiting hormone (GIH) expression in the eyestalks of Litopenaeus vannamei

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