In vivo and in vitro inhibitory action of 17β-estradiol and environmental estrogen 4-nonylphenol on gonad-inhibiting hormone (GIH) expression in the eyestalks of Litopenaeus vannamei

Li, G.L.; Chen, H.P.; Deng, Si‐Ping; Ye, M.; Jiang, Shigui; Chan, S. C.; Zhu, Chunhua

doi:10.4238/2015.october.29.25

Cited by 8 publications

(6 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…vannamei is predominately generated in both the brain and the eyestalk [ 15 , 16 ]. VIH -2 transcript levels have been found to increase significantly as fertilized eggs develop into juveniles, peak at sexual differentiation [ 16 ], under a negative estrogen regulation [ 30 ], and decrease in the brain after unilateral eyestalk ablation [ 15 ]. The recombinant VIH-2 protein is effective in inhibiting the hepatopancreatic Vg mRNA expression in female shrimp by both in vitro primary cell culture and in vivo injection approaches, and in reversing ovarian growth induced by eyestalk ablation [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Mechanisms for type-II vitellogenesis-inhibiting hormone suppression of vitellogenin transcription in shrimp hepatopancreas: Crosstalk of GC/cGMP pathway with different MAPK-dependent cascades

et al. 2018

View full text Add to dashboard Cite

Vitellogenesis is the process of yolk formation via accumulating vitellin (Vn) with nutrients in the oocytes. Expression of vitellogenin (Vg), the precursor of Vn, is one of the indicators for the start of vitellogenesis. In Pacific white shrimp (Litopenaeus vannamei), the type-II vitellogenesis-inhibiting hormone (VIH-2) effectively suppresses hepatopancreatic Vg mRNA expression. In this study, we demonstrate the increasing transcript levels of hepatopancreatic Vg during L. vannamei ovarian development, suggesting that the hepatopancreas-derived Vg/Vn may also contribute to vitellogenesis in this species. Using a combination of in vivo injections and in vitro primary cell cultures, we provide evidences that the inhibition of VIH-2 on hepatopancreatic Vg gene expression is mediated through a functional coupling of the GC/cGMP pathway with different MAPK-dependent cascades in female shrimp. In VIH-2 signaling, the NO-independent GC/cGMP/PKG cascades were upstream of the MAPKs. Activations of the MAPK signal by VIH-2 include the phosphorylation of JNK and the mRNA/protein expression of P38MAPK. Additionally, the cAMP/PKA pathway is another positive intracellular signal for hepatopancreatic Vg mRNA expression but is independent of its VIH-2 regulation. Our findings establish a model for the signal transduction mechanism of Vg regulation by VIH and shed light on the biological functions and signaling of the CHH family in crustaceans.

show abstract

Section: Introductionmentioning

confidence: 99%

Mechanisms for type-II vitellogenesis-inhibiting hormone suppression of vitellogenin transcription in shrimp hepatopancreas: Crosstalk of GC/cGMP pathway with different MAPK-dependent cascades

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Many studies have demonstrated physiological responses of crustaceans to exogenously administered steroid hormones. For example, administration of 17b-estradiol advanced ovarian development (29) and stimulated vitellogenesis in female decapods (28). This compound also suppressed vitellogenesisinhibiting hormone gene-expression, which was presumably responsible for the effects on ovarian development (28).…”

Section: Responses To Exogenous Steroidsmentioning

confidence: 98%

“…For example, administration of 17b-estradiol advanced ovarian development (29) and stimulated vitellogenesis in female decapods (28). This compound also suppressed vitellogenesisinhibiting hormone gene-expression, which was presumably responsible for the effects on ovarian development (28). While 17b-estradiol administration to crayfish stimulated vitellogenin-mRNA accumulation in the hepatopancreas, progesterone administration increased vitellogenin protein levels in the hemolymph of crayfish (157).…”

Section: Responses To Exogenous Steroidsmentioning

confidence: 98%

“…Environmental chemicals that have been classified as EDCs, are, for the most part, compounds that interfere with the vertebrate hormone system. Strenuous effort has been made to demonstrate possible endocrine disrupting effects of such EDCs in crustaceans (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30). Although effects on growth and reproduction are often reported, most studies failed to demonstrate that endocrine mechanisms were involved.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Crab Is Not a Fish: Unique Aspects of the Crustacean Endocrine System and Considerations for Endocrine Toxicology

2021

View full text Add to dashboard Cite

Crustaceans—and arthropods in general—exhibit many unique aspects to their physiology. These include the requirement to moult (ecdysis) in order to grow and reproduce, the ability to change color, and multiple strategies for sexual differentiation. Accordingly, the endocrine regulation of these processes involves hormones, receptors, and enzymes that differ from those utilized by vertebrates and other non-arthropod invertebrates. As a result, environmental chemicals known to disrupt endocrine processes in vertebrates are often not endocrine disruptors in crustaceans; while, chemicals that disrupt endocrine processes in crustaceans are often not endocrine disruptors in vertebrates. In this review, we present an overview of the evolution of the endocrine system of crustaceans, highlight endocrine endpoints known to be a target of disruption by chemicals, and identify other components of endocrine signaling that may prove to be targets of disruption. This review highlights that crustaceans need to be evaluated for endocrine disruption with consideration of their unique endocrine system and not with consideration of the endocrine system of vertebrates.

show abstract

“…This complex thus influences both gonadal development and moulting, consequently affecting organismal growth and development (Chen, Kang, Sultana, & Wilder, 2019; Liu, Kong, Chen, & Zhang, 2018; Sainz‐Hernández, Racotta, Dumas, & Hernández‐López, 2008). The eyestalk and gonads are essential for GFP growth and development, and a synergy reportedly exists between them (Jiang et al., 2018; Li et al., 2015). To the best of our knowledge, transcriptome sequencing analyses to investigate gene expression in the eyestalk and gonads have not been performed to date.…”

Section: Introductionmentioning

confidence: 99%

Identification of differentially expressed genes and signalling pathways to elucidate molecular mechanisms underlying growth differences among the male morphotypes of Macrobrachium rosenbergii

Tang

Xia²,

Cai³

et al. 2020

Aquac Res

View full text Add to dashboard Cite

The giant freshwater prawn (GFP) Macrobrachium rosenbergii is an economically important aquaculture species in China. In culture, it is common for males to show unique morphotypes, which exhibit distinct heterogenous individual growth. Little information is currently available on the molecular mechanisms underlying growth differences among male morphotypes of GFPs. Thus, we herein aimed to identify key genes and signalling pathways to elucidate molecular mechanisms underlying growth differences among the male morphotypes of GFPs. We performed transcriptome analyses using RNA‐Seq to compare the following male morphotypes of GFPs: orange claw males, old blue claw males and small males. In total, 97.44 Gb clean data were obtained, and 1,857,760 unigenes were annotated by alignment with public protein databases. Moreover, we found 2,072 and 22,431 differentially expressed genes (DEGs) from the eyestalk and gonads respectively. Gene ontology analyses revealed that ‘system process’ and ‘multicellular organismal process’ were the most enriched in the eyestalk and gonad tissue samples respectively. Furthermore, Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that pathways associated with gene regulation may play a key role in GFP growth. To summarize, this study provides a valuable repository of novel transcripts for various male morphotypes of GFPs and gives further insights into the molecular mechanisms underlying growth differences in GFPs, which should promote the breeding of commercially cultivated GFPs.

show abstract

In vivo and in vitro inhibitory action of 17β-estradiol and environmental estrogen 4-nonylphenol on gonad-inhibiting hormone (GIH) expression in the eyestalks of Litopenaeus vannamei

Cited by 8 publications

References 25 publications

Mechanisms for type-II vitellogenesis-inhibiting hormone suppression of vitellogenin transcription in shrimp hepatopancreas: Crosstalk of GC/cGMP pathway with different MAPK-dependent cascades

Mechanisms for type-II vitellogenesis-inhibiting hormone suppression of vitellogenin transcription in shrimp hepatopancreas: Crosstalk of GC/cGMP pathway with different MAPK-dependent cascades

A Crab Is Not a Fish: Unique Aspects of the Crustacean Endocrine System and Considerations for Endocrine Toxicology

Identification of differentially expressed genes and signalling pathways to elucidate molecular mechanisms underlying growth differences among the male morphotypes of Macrobrachium rosenbergii

Contact Info

Product

Resources

About