Functional characterization of T cells differentiated <i>in vitro</i> from bone marrow‐derived CD34<sup>+</sup> cells of psoriatic patients with family history

Zhang, Kaiming; Li, Xinhua; Yin, Guohua; Liu, Yufeng; Tang, Xuyuan

doi:10.1111/j.1600-0625.2009.01016.x

Cited by 17 publications

(15 citation statements)

References 38 publications

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“…In vitro culture of psoriatic bone marrow-derived hematopoietic stem cells indicated that the patient-derived hematopoietic cells had decreased colony formation ability [21,24]. CD4+/CD8+ T cells and CD4+CD25+ regulatory T cells derived from psoriatic bone marrow CD34+ cells in vitro have been found to be functionally similar to those circulating and lesional psoriatic T cells [6,7]. These findings suggested that the dysfunctional activity of immunocytes, especially T cells, in psoriatic patients could be traced back to the early development of hematopoietic cells.…”

Section: Discussionmentioning

confidence: 99%

“…We revealed that the differentiated T cells from psoriatic patients showed a higher proliferation rate and larger capacity to secrete Th1 cytokines in response to streptococcal superantigen. Compared to normal controls, the differentiated T cells from psoriatic patients induced overexpression of c- myc and Ki67, but not Bcl-xL, in keratinocytes [6]. In another study, we introduced bone marrow CD34+ cells into CD4+CD25+ T regulatory cells in vitro and found that psoriatic CD34+ cell-derived T regulatory cells were significantly compromised in their ability to proliferate, secrete the cytokines interleukin (IL)-2 and IL-10 in response to streptococcal superantigen Strep-A, and inhibit the proliferation of effector T cells [7].…”

Section: Introductionmentioning

confidence: 99%

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Expression of Notch Receptor and Its Target Gene Hes-1 in Bone Marrow CD34+ Cells from Patients with Psoriasis

Yin

Hou

et al. 2012

Dermatology

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Psoriasis is an autoimmune disease mediated mainly by dysfunctional peripheral blood T cells. Both CD4+/CD8+ T cells and CD4+CD25+ regulatory T cells derived from psoriatic CD34+ bone marrow cells in vitro have been found to be functionally similar to those psoriatic circulating and lesional T cells. Notch signaling participates in diverse cell fate decisions during T cell development and has been reported to influence the proliferation of hematopoietic stem cells and the differentiation of T cells. The purpose of this study was to investigate the expression levels of Notch receptor 1, 2 and its target gene Hes-1 in CD34+ cells from patients with psoriasis. The total RNA and protein of CD34+ cells were extracted, and the mRNA as well as protein expression of Notch1, Notch2 and Hes-1 were investigated using real-time PCR and Western blot assays. We found that the mRNA and protein expression levels of Notch1 and Hes-1 in psoriasis patients were higher compared to normal controls, while the Notch2 mRNA and protein expression levels in psoriasis patients were similar to those of normal controls. The elevated Notch1 and Hes-1 expression levels in psoriatic CD34+ cells might be one reason for the immune disorders which are mainly mediated by T cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Expression of Notch Receptor and Its Target Gene Hes-1 in Bone Marrow CD34+ Cells from Patients with Psoriasis

Yin

Hou

et al. 2012

Dermatology

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“…Although the exact pathogenesis is largely unknown, it is generally believed that pathogenic T cells are activated in patients with psoriasis. For example, bone marrow–derived T cells from patients with psoriasis are more proliferative and produce more IL‐8 and IFN‐γ (8). We hypothesized that tobacco smoke exacerbates Th17‐related skin lesions such as psoriasis via the activation of Th17 and the differentiation of Tcm to Th17.…”

Section: Question Addressedmentioning

confidence: 99%

Tobacco smoke is related to Th17 generation with clinical implications for psoriasis patients

Torii

Saito

Furuhashi

et al. 2011

Experimental Dermatology

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based on the known additive or synergistic activity of AMP (23), it is likely that lactoferrin works together with the other AMP at the skin surface to provide an immune defense. Supporting InformationAdditional Supporting Information may be found in the online version of this article: Data S1. Functional classification of sweat proteins. Table S1. Proteome list from sweat by LC-MS ⁄ MS. Please note: Wiley-Blackwell is not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article. Tobacco smoke is related to Th17 generation with clinical implications for psoriasis patientsKan Torii, Chiyo Saito, Takuya Furuhashi, Akiko Nishioka, Yoichi Shintani, Kana Kawashima, Hiroshi Kato and Akimichi Morita Department of Geriatric and Environmental Dermatology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan Correspondence: Kan Torii, Department of Geriatric and Environmental Dermatology, Nagoya City University Graduate School of Medical Sciences, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya 467-8601, Japan, Tel.: +81-52-853-8261, Fax: +81-52-852-5449, e-mail: kan042033@live.jp Abstract: Environmental factors contribute to the increased prevalence of autoimmune diseases via T helper type-17 cell (Th17) activation. Tobacco smoking increases the risk of psoriasis, but the mechanisms are not clear. We evaluated the percentage of circulating Th17 among CD3 + cells in peripheral blood mononuclear cells (PBMC) obtained from 27 healthy volunteers (2.58 ± 0.80%), 33 smoker (3.55 ± 1.33%) and 21 non-smoker (3.10 ± 1.14%) patients with psoriasis to elucidate the relation between smoking and psoriasis. More smokers (19 ⁄ 33) than non-smokers (6 ⁄ 21) had high Th17 levels (Th17 ⁄ CD3 > 3.38%, mean + 1 SD of healthy volunteers). Tobacco smoke extract (TSE, 7 ll ⁄ ml) induced Th17 generation from central memory T cells in vitro. TSE increased interleukin 17 and 22 expression. These findings demonstrate the relation between tobacco smoke and IL-17 and IL-22, which exacerbate psoriasis.

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“…In psoriasis, the level of the Thelper 1 cytokine is enhanced in T cells directly differentiated from psoriatic bone marrow CD34 + cells in vitro, and the number and function of regulatory T cells is decreased. 3,4 Moreover, expression of the T-cell receptor variable region is predominant in peripheral blood T-cell of psoriasis. 5 Additionally, it was found that psoriatic T cells influence keratinocyte proliferation.…”

Section: Introductionmentioning

confidence: 99%

Psoriatic T cells reduce epidermal turnover time and affect cell proliferation contributed from differential gene expression

Hou

et al. 2015

The Journal of Dermatology

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Psoriasis is mediated primarily by T cells, which reduce epidermal turnover time and affect keratinocyte proliferation. We aimed to identify differentially expressed genes (DEG) in T cells from normal, five pairs of monozygotic twins concordant or discordant for psoriasis, to determine whether these DEG may account for the influence to epidermal turnover time and keratinocyte proliferation. The impact of T cells on keratinocyte proliferation and epidermal turnover time were investigated separately by immunohistochemistry and cultured with 3 H-TdR. mRNA expression patterns were investigated by RNA sequencing and verified by real-time reverse transcription polymerase chain reaction. After co-culture with psoriatic T cells, the expression of Ki-67, c-Myc and p53 increased, while expression of Bcl-2 and epidermal turnover time decreased. There were 14 DEG which were found to participate in the regulation of cell proliferation or differentiation. Psoriatic T cells exhibited the ability to decrease epidermal turnover time and affect keratinocyte proliferation because of the differential expression of PPIL1, HSPH1, SENP3, NUP54, FABP5, PLEKHG3, SLC9A9 and CHCHD4.

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Functional characterization of T cells differentiated in vitro from bone marrow‐derived CD34⁺ cells of psoriatic patients with family history

Cited by 17 publications

References 38 publications

Expression of Notch Receptor and Its Target Gene Hes-1 in Bone Marrow CD34+ Cells from Patients with Psoriasis

Expression of Notch Receptor and Its Target Gene Hes-1 in Bone Marrow CD34+ Cells from Patients with Psoriasis

Tobacco smoke is related to Th17 generation with clinical implications for psoriasis patients

Psoriatic T cells reduce epidermal turnover time and affect cell proliferation contributed from differential gene expression

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Functional characterization of T cells differentiated in vitro from bone marrow‐derived CD34+ cells of psoriatic patients with family history

Cited by 17 publications

References 38 publications

Expression of Notch Receptor and Its Target Gene Hes-1 in Bone Marrow CD34+ Cells from Patients with Psoriasis

Expression of Notch Receptor and Its Target Gene Hes-1 in Bone Marrow CD34+ Cells from Patients with Psoriasis

Tobacco smoke is related to Th17 generation with clinical implications for psoriasis patients

Psoriatic T cells reduce epidermal turnover time and affect cell proliferation contributed from differential gene expression

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Functional characterization of T cells differentiated in vitro from bone marrow‐derived CD34⁺ cells of psoriatic patients with family history