Functional characterization of the promoter of the vitellogenin gene, AsVg1, of the malaria vector, Anopheles stephensi

Transgenesis, Paratransgenesis and Transmission Blocking Vaccines to Prevent Insect-Borne Diseases

“…The same promoter was used to express CFP in An. stephensi (Nirmala et al 2006). The robustness of the vitellogenin promoter was confirmed by its capacity to function following multiple gonotrophic cycles in transgenic An.…”

“…aegypti. Alternatively, phenotypic markers such as firefly luciferase, or green (EGFP), red (dsRED) and cyan fluorescent (CFP) proteins can be used as transformation markers (Moreira et al 2000;Kokoza et al 2001;Nolan et al 2002;Perera et al 2002;Nirmala et al 2006).…”

Gene Expression Studies in Mosquitoes

“…The size of the control DNA is consistent with prior observations in transgenesis studies with an An. stephensi vitellogenin-encoding gene promoter (Nirmala et al, 2006). Functional mapping of the Ae.…”

“…Control sequences of AsVgl directed blood meal stimulated sex-and tissue-specific expression of a reporter gene in transgenic adult female An. stephensi (Nirmala et al, 2006). DNA fragments encompassing the 850 bp immediately adjacent to the 50-end of the gene and the 30-end untranslated region are sufficient to direct this expression.…”

Perspectives in the control of infectious diseases by transgenic mosquitoes in the post-genomic era: a review

“…More recently, the vitellogenin promoter sequence has been obtained from An. stephensi and showed to have its expression regulated in a sex-, stage-and tissue specific manner, and therefore is a good candidate to express transgenes in anophelines (Nirmala et al 2006).…”