Functional Characterization of the Putative <i>Aspergillus nidulans</i> Poly(ADP-Ribose) Polymerase Homolog PrpA

Semighini, Camile P.; Savoldi, Marcela; Goldman, Gustavo H.; Harris, Steven D.

doi:10.1534/genetics.105.053199

Cited by 52 publications

(45 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This assay uses terminal deoxynucleotidyltransferase to label 3 0 -OH DNA termini with TMR-conjugated dUTP, which can be directly visualized by fluorescence microscopy. As previously shown by Semighini et al (2006), when germlings from the wild type strain were treated with 100 lM FOH for 2 h, about 50% showed TUNEL positive staining, whereas untreated control hyphae showed no staining ( Supplementary Fig. 3S).…”

Section: The External Alternative Nadh Dehydrogenase Ndea Is Involvedsupporting

confidence: 77%

The roles played by Aspergillus nidulans apoptosis-inducing factor (AIF)-like mitochondrial oxidoreductase (AifA) and NADH-ubiquinone oxidoreductases (NdeA-B and NdiA) in farnesol resistance

Dinamarco

Pimentel

Savoldi

et al. 2010

Fungal Genetics and Biology

Self Cite

View full text Add to dashboard Cite

Farnesol (FOH) is a nonsterol isoprenoid produced by dephosphorylation of farnesyl pyrophosphate, a catabolite of the cholesterol biosynthetic pathway. These isoprenoids inhibit proliferation and induce apoptosis. Here, we show that Aspergillus nidulans AifA encoding the apoptosis-inducing factor (AIF)-like mitochondrial oxidoreductase plays a role in the function of the mitochondrial Complex I. Additionally, we demonstrated that ndeA-B and ndiA encode external and internal alternative NADH dehydrogenases, respectively, that have a function in FOH resistance. When exposed to FOH, the ΔaifA and ΔndeA strains have increased ROS production while ΔndeB, ΔndeA ΔndeB, and ΔndiA mutant strains showed the same ROS accumulation than in the absence of FOH. We observed several compensatory mechanisms affecting the differential survival of these mutants to FOH.

show abstract

Section: The External Alternative Nadh Dehydrogenase Ndea Is Involvedsupporting

confidence: 77%

The roles played by Aspergillus nidulans apoptosis-inducing factor (AIF)-like mitochondrial oxidoreductase (AifA) and NADH-ubiquinone oxidoreductases (NdeA-B and NdiA) in farnesol resistance

Dinamarco

Pimentel

Savoldi

et al. 2010

Fungal Genetics and Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…HsPARP1 orthologs have been shown to be involved or implicated in DNA repair in animals [8,118,137], fungi [81,126], Trypanosoma cruzi [46] and Dictyostelium discoideum [112]. It is likely that a role in DNA repair is shared by most orthologs of HsPARP1, HsPARP2 and HsPARP3.…”

Section: Functions Of Parp Superfamily Members In Plantsmentioning

confidence: 99%

Functions of the poly(ADP-ribose) polymerase superfamily in plants

Lamb

Citarelli

Teotia

2011

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Poly(ADP-ribosyl)ation is the covalent attachment of ADP-ribose subunits from NAD(+) to target proteins and was first described in plants in the 1970s. This post-translational modification is mediated by poly(ADP-ribose) polymerases (PARPs) and removed by poly(ADP-ribose) glycohydrolases (PARGs). PARPs have important functions in many biological processes including DNA repair, epigenetic regulation and transcription. However, these roles are not always associated with enzymatic activity. The PARP superfamily has been well studied in animals, but remains under-investigated in plants. Although plants lack the variety of PARP superfamily members found in mammals, they do encode three different types of PARP superfamily proteins, including a group of PARP-like proteins, the SRO family, that are plant specific. In plants, members of the PARP family and/or poly(ADP-ribosyl)ation have been linked to DNA repair, mitosis, innate immunity and stress responses. In addition, members of the SRO family have been shown to be necessary for normal sporophytic development. In this review, we summarize the current state of plant research into poly(ADP-ribosyl)ation and the PARP superfamily in plants.

show abstract

“…As part of our effort to characterize features of the DNA damage response in A. nidulans (Goldman et al 2002), we recently described homologs of two key signaling components of the animal DNA damage response: AtmA (ataxia telangiectasia mutated, or ATM) and PrpA [poly(ADP)-ribose polymerase, or PARP] (Malavazi et al 2006;Semighini et al 2006). Notably, in addition to the expected defects in the DNA damage response, mutations in atmA and prpA also affected morphogenesis and development.…”

mentioning

confidence: 99%

Regulation of Apical Dominance inAspergillus nidulansHyphae by Reactive Oxygen Species

Semighini¹,

2008

Self Cite

View full text Add to dashboard Cite

In fungal hyphae, apical dominance refers to the suppression of secondary polarity axes in the general vicinity of a growing hyphal tip. The mechanisms underlying apical dominance remain largely undefined, although calcium signaling may play a role. Here, we describe the localized accumulation of reactive oxygen species (ROS) in the apical region of Aspergillus nidulans hyphae. Our analysis of atmA (ATM) and prpA (PARP) mutants reveals a correlation between localized production of ROS and enforcement of apical dominance. We also provide evidence that NADPH oxidase (Nox) or related flavoproteins are responsible for the generation of ROS at hyphal tips and characterize the roles of the potential Nox regulators NoxR, Rac1, and Cdc42 in this process. Notably, our genetic analyses suggest that Rac1 activates Nox, whereas NoxR and Cdc42 may function together in a parallel pathway that regulates Nox localization. Moreover, the latter pathway may also include Bem1, which we propose represents a p40phox analog in fungi. Collectively, our results support a model whereby localized Nox activity generates a pool of ROS that defines a dominant polarity axis at hyphal tips.

show abstract

Functional Characterization of the Putative Aspergillus nidulans Poly(ADP-Ribose) Polymerase Homolog PrpA

Cited by 52 publications

References 65 publications

The roles played by Aspergillus nidulans apoptosis-inducing factor (AIF)-like mitochondrial oxidoreductase (AifA) and NADH-ubiquinone oxidoreductases (NdeA-B and NdiA) in farnesol resistance

The roles played by Aspergillus nidulans apoptosis-inducing factor (AIF)-like mitochondrial oxidoreductase (AifA) and NADH-ubiquinone oxidoreductases (NdeA-B and NdiA) in farnesol resistance

Functions of the poly(ADP-ribose) polymerase superfamily in plants

Regulation of Apical Dominance inAspergillus nidulansHyphae by Reactive Oxygen Species

Contact Info

Product

Resources

About