Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer

Stewart, Walter W.

doi:10.1016/0092-8674(78)90256-8

Cited by 1,277 publications

(452 citation statements)

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“…Of those tested AB was most convenient. At 5 M in tration inhibits the production of nucleated cells for 4 weeks complete culture medium it blocks functional gap junctions, and inhibits CFU-c production moderately during the second tested by lucifer yellow dye transfer, 19 between FBMD-1 stroand third weeks. CFU-c formation by all subsets (Figure 4a).…”

Section: Resultsmentioning

confidence: 99%

“…Once growth was established the clones used in Table 1 were plated at limiting dilutions Gap junction coupling in stromal cell lines was measured by and selected on the basis of their showing steady, nontransformicro-injecting lucifer yellow, MW 457, 12,19 and counting the med growth and possessing the morphology of large polynumber of cells to which it spread. Stromal cells were cultured gonal pre-adipocytic cells with large nuclei.…”

Section: Stromal Cell Linesmentioning

confidence: 99%

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Does transmembrane communication through gap junctions enable stem cells to overcome stromal inhibition?

et al. 1997

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ronectin on stromal cells. 11 Lipophilic compounds that do not affect GJIC had no effect onWe asked if the gap junctions which are present in haemo- drugs (Ref. 13 and Krenács and Rosendaal, in preparation).Keywords: blood formation; haemopoietic regulation; gap junctions; Cx43; Amphotericin B; stem cells; stroma Gap junctions are intramembranous channels through the walls of adjacent cells which permit the passage of watersoluble molecules smaller than 1 kDa, which could be messengers. Channels are gated, open when cytosolic [Ca 2+ i ] is Introduction high or pH low and closed when the opposite conditions occur. A single gap junction channel is called a connexon Normally blood cells form only in those parts of the body, and is formed by two hemi-connexons, one in each cell wall. especially bone marrow, where specialised cells calledThe channels group into plaques and these are the functional haemopoietic stromal cells are found. Grafting these cells into gap junctions. Each hemi-connexon is formed by six identical, non-medullary tissues such as the kidney enables such tissues membrane-spanning proteins called connexins. Thirteen conto form blood. 1 We have been able to understand many nexin species have been described and named for their molaspects of lifelong blood formation supported by stromal cells ecular weights in thousands, abbreviated as Cx43, etc. 15 Cerusing long-term bone marrow cultures. 2 tain kinds of stromal cell in haemopoietic tissue are coupled It was thought that long-term cultures required an underlyby Cx43 gap junctions, but we do not know yet if that is the ing monolayer of adherent bone marrow stromal cells to form only connexin species that can form haemopoietic junctions. blood cells for a long time. Bentley 3 indicated that stroma and There are functional gap junctions in long-term cultures stem cells need only be close for the maintenance of spleen between stromal and haemopoietic cells. 12 To investigate a colony-forming units and Brandt and colleagues 4 found that possible role of gap junctions in the regulation of haemopothe underlay could be replaced by adding recombinant iesis we studied the numerical alterations in clone formation interleukins IL-1 alpha, IL-3, IL-6, or granulocyte-macrophage and progenitor cell generation in stroma-supported long-term colony-stimulating factor (GM-CSF) at 2-day intervals. IL-3 cultures 2,[16][17][18] in the presence of lipophilic substances which block GJIC. Absolute numbers of haemopoietic clones were estimated using the cobblestone area forming cell (CAFC) culture carried out in a limited dilution set-up. In the mouse

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Section: Resultsmentioning

confidence: 99%

Section: Stromal Cell Linesmentioning

confidence: 99%

Does transmembrane communication through gap junctions enable stem cells to overcome stromal inhibition?

et al. 1997

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“…Microelectrodes were backfilled with a 60 mM KC1 solution containing 8% potassium Lucifer yellow (LY) (Molecular Probes, Inc., Eugene, OR). This particular fluorescent dye was chosen for these studies as it is stable to fixation and thus would allow the dye spread pattern to be further analyzed by thick section histology (Stewart, 1978; see below). Impalements were carried out under a Leitz Diavert microscope using Leitz micromanipulators.…”

Section: Dye Coupling Studiesmentioning

confidence: 99%

Restrictions in gap junctional communication in the Drosophila larval epidermis

Ruangvoravat

1992

Developmental Dynamics

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We characterized gap junctional communication in the Drosophila larval epidermis by monitoring the pattern of dye spread following the intracellular injection of the fluorescent dye, Lucifer yellow. We found that dye injected into the epidermis spread extensively from cell to cell, but at segment borders and also at boundaries positioned at the lateral aspects of each body segment, dye spread was restricted. The precise position of these boundaries of restricted gap junctional communication was determined by examining the distribution of the fluorescent tracer in thick sections of each dyeinjected specimen. These results show that each of the thoracic and abdominal segments is segregated into four domains or communication compartments. We also observed the presence of dye in the procuticle and epicuticle, and examined the possible basis for this dye localization.

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“…Their somata are located near the surface of the distal end of the optic tract (Okada and Yamaguchi 1988). For intracellular recording and current injection, quartz glass microelectrodes filled with 3% solution of Lucifer yellow CH (Stewart 1978) in 1M lithium chloride were employed. The NGI was impaled with the microelectrode on or near the midline.…”

Section: Intracellular Recording and Current Injectionmentioning

confidence: 99%

Physiological changes of premotor nonspiking interneurons in the central compensation of eyestalk posture following unilateral sensory ablation in crayfish

Fujisawa

Takahata

2006

J Comp Physiol A

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AbstractsWe investigated how the physiological characteristics and synaptic activities of NGIs (Nonspiking Giant Interneurons), which integrate sensory inputs in the brain and send synaptic outputs to oculomotor neurons innervating eyestalk muscles, changed after unilateral ablation of the statocyst in order to clarify neuronal mechanisms underlying the central compensation process in crayfish. The input resistance and membrane time constant in recovered animals that restored the original symmetrical eyestalk posture two weeks after operation were significantly greater than those immediately after operation on the operated side whereas in non-recovered animals only the membrane time constant showed a significant increase. On the intact side, both recovered and non-recovered animals showed no difference. The frequency of synaptic activity showed a complex pattern of change on both sides depending on the polarity of the synaptic potential. The synaptic activity returned to the bilaterally symmetrical level in recovered animals while bilateral asymmetry remained in non-recovered ones. These results suggest that the central compensation of eyestalk posture following unilateral impairment of the statocyst is subserved by not only changes in the physiological characteristics of the NGI membrane but also the activity of neuronal circuits presynaptic to NGIs.Fujisawa and Takahata -3-

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Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer

Cited by 1,277 publications

References 30 publications

Does transmembrane communication through gap junctions enable stem cells to overcome stromal inhibition?

Does transmembrane communication through gap junctions enable stem cells to overcome stromal inhibition?

Restrictions in gap junctional communication in the Drosophila larval epidermis

Physiological changes of premotor nonspiking interneurons in the central compensation of eyestalk posture following unilateral sensory ablation in crayfish

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