Functional conservation and divergence of SEPALLATA-like genes in the development of two-type florets in marigold

Zhang, Chunyu; Wei, Ludan; Yu, Xiaomin; Li, Hang; Wang, Wenjing; Wu, Shenzhong; Duan, Fengying; Bao, Manzhu; Chan, Zhulong; He, Yan‐Hong

doi:10.1016/j.plantsci.2021.110938

Cited by 6 publications

(6 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most of the studies have identified transcription factors positively regulating artemisinin biosynthesis whereas negative regulators are hardly reported. Wu et al ( 2021 ) identified an R2R3- MYB transcription factor AaMYB15 in A. annua and reported that its over-expression results in decreased expression of certain key genes ADS , CYP , DBR2 , and ALDH1 . It binds the promoter of AaORA and represses its activity, which indirectly results in decreased artemisinin content.…”

Section: Artemisinin Enhancement Strategiesmentioning

confidence: 99%

Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches

Wani

Choudhary

Zehra

et al. 2021

Planta

View full text Add to dashboard Cite

show abstract

Section: Artemisinin Enhancement Strategiesmentioning

confidence: 99%

Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches

Wani

Choudhary

Zehra

et al. 2021

Planta

View full text Add to dashboard Cite

show abstract

“…In contrast, in Phalaenopsis , PeSEP2 is highly expressed in the column, PeSEP3 expression is dominant in the petal, and PeSEP4 expression is extremely low in floral organs ( Pan et al., 2014 ). SEP3 homologous and SEP4 homologous genes are mainly highly expressed in the outer two whorls of marigold and gerbera ( Zhang T. et al., 2017 ; Zhang et al., 2021 ). The non-ubiquitous expression patterns of SEPALLATA -like genes offered a rare opportunity to investigate their functional conservation and divergence in C. sinense .…”

Section: Discussionmentioning

confidence: 99%

“…Considering that the SEP homologous genes in different angiosperms are diverse, leading to the homeotic conversion of floral organs and even affecting fruit development ( Zhang S. et al., 2017 ; Qi et al., 2020 ; Zhang et al., 2021 ; Zhang et al., 2022 ). For example, SEP homologous genes in Isatis indigotica , Marigold , and Gerbera can affect the structure of sepals, petals, stamens and carpels ( Uimari et al., 2004 ; Ma et al., 2019 ; Zhang et al., 2021 ). Additionally, SEP homologous genes can effect fruit and seed also have reported in grapevine ( Zhang et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…The phenotypic changes in transgenic plants were verified to be related to the expression levels of their endogenous genes regulated by SEP genes. For instance, in marigold and Platanus acerifolia , ectopic expression of SEP homologous genes regulates the expression of endogenous genes in A. thaliana ( Zhang S. et al., 2017 ; Zhang et al., 2021 ). Consistent with those conclusions, the expression levels of endogenous floral development genes, such as AtFT , AtLFY , AtSEP3 , AtSOC1 and AtAG , were significantly upregulated in 35S: CsSEP transgenic plants ( Figure 7 ), and the phenotypic differences might be due to the different capabilities of activating downstream target genes in Arabidopsis.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Functional conservation and divergence of SEPALLATA-like genes in floral development in Cymbidium sinense

Lin,

Zhu,

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Cymbidium sinense is one of the most important traditional Chinese Orchids due to its unique and highly ornamental floral organs. Although the ABCDE model for flower development is well-established in model plant species, the precise roles of these genes in C. sinense are not yet fully understood. In this study, four SEPALLATA-like genes were isolated and identified from C. sinense. CsSEP1 and CsSEP3 were grouped into the AGL9 clade, while CsSEP2 and CsSEP4 were included in the AGL2/3/4 clade. The expression pattern of CsSEP genes showed that they were significantly accumulated in reproductive tissues and expressed during flower bud development but only mildly detected or even undetected in vegetative organs. Subcellular localization revealed that CsSEP1 and CsSEP4 were localized to the nucleus, while CsSEP2 and CsSEP3 were located at the nuclear membrane. Promoter sequence analysis predicted that CsSEP genes contained a number of hormone response elements (HREs) and MADS-box binding sites. The early flowering phenotype observed in transgenic Arabidopsis plants expressing four CsSEP genes, along with the expression profiles of endogenous genes, such as SOC1, LFY, AG, FT, SEP3 and TCPs, in both transgenic Arabidopsis and C. sinense protoplasts, suggested that the CsSEP genes played a regulatory role in the flowering transition by influencing downstream genes related to flowering. However, only transgenic plants overexpressing CsSEP3 and CsSEP4 caused abnormal phenotypes of floral organs, while CsSEP1 and CsSEP2 had no effect on floral organs. Protein-protein interaction assays indicated that CsSEPs formed a protein complex with B-class CsAP3-2 and CsSOC1 proteins, affecting downstream genes to regulate floral organs and flowering time. Our findings highlighted both the functional conservation and divergence of SEPALLATA-like genes in C. sinense floral development. These results provided a valuable foundation for future studies of the molecular network underlying floral development in C. sinense.

show abstract

“…These characteristics make marigold a valuable material for studying the molecular mechanism of marigold inflorescence formation. In our previous work, we have obtained functionally characterized class B (TePI, TeAP3-1, TeAP3-2, TeTM6-1, and TeTM6-2) [ 49 ], C (TeAG1 and TeAG2) [ 50 ], D (TeAGL11-1 and TeAGL11-2) [ 50 ], and E (TeSEP1, TeSEP3-1, TeSEP3-2, TeSEP3-3, and TeSEP4) [ 51 ] genes, which are active during marigold inflorescence and floret development and have specific expression patterns in floral organs. In this study, we cloned and characterized five AP1/FUL -like genes in marigold, whose distinct expression patterns, protein interaction patterns, and different phenotypes in Arabidopsis transgenic lines might imply divergent functions of these five genes in regulating the floral meristem development, floral organ identity, and flowering time.…”

Section: Introductionmentioning

confidence: 99%

Functional Conservation and Divergence of Five AP1/FUL-like Genes in Marigold (Tagetes erecta L.)

Zhang

Sun

et al. 2021

Genes

Self Cite

View full text Add to dashboard Cite

Members of AP1/FUL subfamily genes play an essential role in the regulation of floral meristem transition, floral organ identity, and fruit ripping. At present, there have been insufficient studies to explain the function of the AP1/FUL-like subfamily genes in Asteraceae. Here, we cloned two euAP1 clade genes TeAP1-1 and TeAP1-2, and three euFUL clade genes TeFUL1, TeFUL2, and TeFUL3 from marigold (Tagetes erecta L.). Expression profile analysis demonstrated that TeAP1-1 and TeAP1-2 were mainly expressed in receptacles, sepals, petals, and ovules. TeFUL1 and TeFUL3 were expressed in flower buds, stems, and leaves, as well as reproductive tissues, while TeFUL2 was mainly expressed in flower buds and vegetative tissues. Overexpression of TeAP1-2 or TeFUL2 in Arabidopsis resulted in early flowering, implying that these two genes might regulate the floral transition. Yeast two-hybrid analysis indicated that TeAP1/FUL proteins only interacted with TeSEP proteins to form heterodimers and that TeFUL2 could also form a homodimer. In general, TeAP1-1 and TeAP1-2 might play a conserved role in regulating sepal and petal identity, similar to the functions of MADS-box class A genes, while TeFUL genes might display divergent functions. This study provides a theoretical basis for the study of AP1/FUL-like genes in Asteraceae species.

show abstract

Functional conservation and divergence of SEPALLATA-like genes in the development of two-type florets in marigold

Cited by 6 publications

References 77 publications

Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches

Enhancing artemisinin content in and delivery from Artemisia annua: a review of alternative, classical, and transgenic approaches

Functional conservation and divergence of SEPALLATA-like genes in floral development in Cymbidium sinense

Functional Conservation and Divergence of Five AP1/FUL-like Genes in Marigold (Tagetes erecta L.)

Contact Info

Product

Resources

About