Functional cytochrome oxidase histochemistry in the honeybee brain

Armengaud, Catherine; Caussé, Nicolas; Aitoubah, Jamila; Ginolhac, Aurélien; Gauthier, M.

doi:10.1016/s0006-8993(00)02016-3

Cited by 22 publications

(18 citation statements)

References 13 publications

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“…The complex effect of imidacloprid on neuronal metabolism has also been observed after intracranial injection of the insecticide in the honeybee. In the mushroom bodies, the CO activity is decreased by imidacloprid at 10 M corresponding to 25 ng/bee (Armengaud et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…In the mushroom bodies of the honeybee brain, the antagonists induce a reduction of the activity of cytochrome oxidase (CO), a mitochondrial enzyme whose activity is used as an indicator of neuronal metabolism ). Imidacloprid at 10 -4 M induces an increase of CO staining in the same structures, indicating brain metabolism activation (Armengaud et al, 2000). In this context, we make the hypothesis that nicotinic agonists, like imidacloprid at sublethal doses that do not affect the global behavior of the honeybee, can improve learning abilities.…”

Section: Introductionmentioning

confidence: 91%

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Imidacloprid‐induced facilitation of the proboscis extension reflex habituation in the honeybee

Lambin

Armengaud

Raymond

et al. 2001

Arch Insect Biochem Physiol

160

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Imidacloprid is a new insecticide from the family of the neonicotinoids, which interact with the insect nicotinic acetylcholine receptor. The effects of imidacloprid at a dose that does not affect sensory or motor functions are studied on non-associative learning abilities in the honeybee. The behavioral procedure is the habituation of the proboscis extension reflex (PER). Imidacloprid topically applied on the thorax (1 microl) at the doses of 5, 10, and 20 ng/bee induces an increase of the gustatory threshold defined as the lowest concentration of a sucrose solution applied to the antennae able to elicit the PER. The ability of the honeybee to move in an open-field-like apparatus is impaired at the doses of 2.5, 5, 10, and 20 ng/bee. These effects are amplified with time and reach a maximum 60 min after application. The lowest dose of 1.25 ng/bee has no effect on the gustatory function but increases the motor activity and facilitates the PER habituation independently of time. This result fits our hypothesis that a slight activation of the cholinergic system with a low dose of imidacloprid can facilitate a simple form of learning in the honeybee.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

Imidacloprid‐induced facilitation of the proboscis extension reflex habituation in the honeybee

Lambin

Armengaud

Raymond

et al. 2001

Arch Insect Biochem Physiol

160

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show abstract

“…41 The structure-specific increase of cytochrome oxidase activity into the brain that is observed after treatment with imidacloprid suggests an impairment of olfactory memory provoked by a physiological effect at the higher cerebral level, in the area called "mushroom bodies". 41,43 But, the precise consequences of these results for the foraging behavior are still unclear. There is a problem in how the results can be extrapolated to what should happen in realistic field situation.…”

Section: Learning Performancesmentioning

confidence: 99%

Ecotoxicity of Neonicotinoid Insecticides to Bees

Decourtye¹,

Devillers²

2010

Advances in Experimental Medicine and Biology

184

119

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T his chapter reviews the available data on the toxicity of neonicotinoid insecticides to bees that are the prominent and the most economically important group of pollinators worldwide. Classical and new methods developed to take into account the characteristics and different types of effects of the neonicotinoid insecticides to bees are described. The available toxicity results are critically analyzed. Thus, the nitro-substituted compounds (clothianidin, dinotefuran, imidacloprid and its metabolites, thiamethoxam, nitenpyram) appear the most toxic to bees. The cyano-substituted neonicotinoids seem to exhibit a much lower toxicity (acetamiprid and thiacloprid). The chapter ends with suggestions for additional studies aiming at better assess the hazard of this important insecticide family to bees.

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“…In epilepsy-prone mice, increased COX I expression is associated with the epileptogenesis as a consequence of mitochondrial hyperactivity (Nakagawa et al 2002). COX activity has also been linked to experience-related neuronal activity in the honeybee brain (Armengaud et al 2000). Antennal input deprivation leads to reduced COX activity staining in three layers of the vertical lobe of the mushroom body.…”

Section: Figurementioning

confidence: 99%

“…Antennal input deprivation leads to reduced COX activity staining in three layers of the vertical lobe of the mushroom body. Furthermore, chemical stimulation with potassium ions to cause global depolarization in neurons resulted in significantly increased COX activity in the antennal lobe of the bee (Armengaud et al 2000). Altered COX activity has been encountered in task-relevant regions of the cattlefish brain after learning (Agin et al 2001).…”

Section: Figurementioning

confidence: 99%

Memory consolidation and gene expression in Periplaneta americana

Pintér¹

2005

Learn. Mem.

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A unique behavioral paradigm has been developed for Periplaneta americana that assesses the timing and success of memory consolidation leading to long-term memory of visual-olfactory associations. The brains of trained and control animals, removed at the critical consolidation period, were screened by two-directional suppression subtractive hybridization. Screens identified neurobiologically relevant as well as novel genes that are differentially expressed at the consolidation phase of memory. The differential expression of six transcripts was confirmed with real-time RT-PCR experiments. There are mitochondrial DNA encoded transcripts among the up-regulated ones (COX, ATPase6). One of the confirmed down-regulated transcripts is RNA polymerase II largest subunit. The mitochondrial genes are of particular interest because mitochondria represent autonomous DNA at synapses. These transcripts will be used as one of several tools in the identification of neuronal circuits, such as in the mushroom bodies, that are implicated in memory consolidation.

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Functional cytochrome oxidase histochemistry in the honeybee brain

Cited by 22 publications

References 13 publications

Imidacloprid‐induced facilitation of the proboscis extension reflex habituation in the honeybee

Imidacloprid‐induced facilitation of the proboscis extension reflex habituation in the honeybee

Ecotoxicity of Neonicotinoid Insecticides to Bees

Memory consolidation and gene expression in Periplaneta americana

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